Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,148,750 T→C 5.7% intergenic (+59/‑9) plsX → / → fabH putative phosphate acyltransferase/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,148,7500TC 5.7% 214.1 / 2.9 70intergenic (+59/‑9)plsX/fabHputative phosphate acyltransferase/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base T (30/36);  new base C (2/2);  total (32/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCG  >  NC_000913/1148602‑1148896
                                                                                                                                                    |                                                                                                                                                  
gTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt                                                                                                                                                    >  2:397419/1‑149 (MQ=255)
gTTCCTCAGCGAATTGCCGCTCGCCGGGAATCTGTATACCCAGCTGGGTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt                                                                                                                                                    <  4:351326/149‑1 (MQ=255)
   ccTCTGCGAATTGCCTCGCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTTGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGa                                                                                                                                                  <  2:269026/148‑1 (MQ=255)
    cTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGc                                                                                                                                                <  1:289501/149‑1 (MQ=255)
    cTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGc                                                                                                                                                >  1:301104/1‑149 (MQ=255)
    cTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGAAGGTATATAACCGAAAAGTGACTGAg                                                                                                                                                 <  4:170826/148‑1 (MQ=255)
       aGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                                                                                                             >  2:9415/1‑149 (MQ=255)
          gAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACAt                                                                                                                                          <  7:62206/149‑1 (MQ=255)
                  gCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACg                                                                                                                                   >  5:146859/1‑148 (MQ=255)
                      gCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGATCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGat                                                                                                                              >  8:128709/1‑149 (MQ=255)
                        cTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGATTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGataa                                                                                                                            >  8:66892/1‑147 (MQ=255)
                         tGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGattat                                                                                                                           >  6:30040/1‑149 (MQ=255)
                          ggAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGattatt                                                                                                                          >  5:238778/1‑149 (MQ=255)
                           gAATCTGTATACCCCGCTGGTTTGGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTg                                                                                                                         <  6:301517/149‑1 (MQ=255)
                           gAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGattatt                                                                                                                          <  8:74411/148‑1 (MQ=255)
                           gAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGattatt                                                                                                                          <  6:248010/148‑1 (MQ=255)
                           gAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGattat                                                                                                                           <  3:320304/147‑1 (MQ=255)
                           gAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGattat                                                                                                                           <  8:232099/147‑1 (MQ=255)
                           gAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTg                                                                                                                         <  7:252302/149‑1 (MQ=255)
                            aaTCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTgg                                                                                                                        <  1:371537/149‑1 (MQ=255)
                                       ccAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGAAGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGc                                                                                                              >  7:124292/1‑148 (MQ=255)
                                         aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAt                                                                                                           <  3:17519/149‑1 (MQ=255)
                                               tcttGAGCTGCTGGACGGTGGCTAAAGCGGAACTCTGCGGTAGCAGGTCGCTGCCAGCGTACTCGCAGTTTGCAAGTGACGGTATTTAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTTTCTGccc                                                                                                     <  8:145733/147‑1 (MQ=255)
                                                tttGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGccc                                                                                                     >  2:137799/1‑148 (MQ=255)
                                                 ttGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCg                                                                                                    <  2:301104/148‑1 (MQ=255)
                                                     gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACaa                                                                                               <  1:9415/149‑1 (MQ=255)
                                                         cttgACTGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAATTGACTGAGCGTACATGTATTCGAAGATTTTTGGTACTGGCAGGTATCTGCCCGATCAAGt                                                                                             <  8:79620/144‑1 (MQ=255)
                                                         ctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                                                                           <  1:15001/149‑1 (MQ=255)
                                                         ctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGc                                                                                           >  2:15001/1‑149 (MQ=255)
                                                                 tGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTATGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGTCAGCTATCTGCCCGAACAAGTGCGGACAAAc                                                                                   >  8:247512/1‑149 (MQ=255)
                                                                   gCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGc                                                                                 <  4:248269/149‑1 (MQ=255)
                                                                    cAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGcc                                                                                >  4:39571/1‑149 (MQ=255)
                                                                     aaaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGcc                                                                                >  6:360015/1‑148 (MQ=255)
                                                                     aaaaGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGcc                                                                                >  2:480/1‑148 (MQ=255)
                                                                          cGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTg                                                                          <  3:333461/149‑1 (MQ=255)
                                                                            gAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGa                                                                        <  1:340858/149‑1 (MQ=255)
                                                                             aaCTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCAGAACAAGTGCGGACAAACGCCGATTTGGa                                                                        >  1:235849/1‑148 (MQ=255)
                                                                                    cGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAAtgg                                                                <  5:30040/149‑1 (MQ=255)
                                                                                     ggTAGCAGGACGCTGCCAGCTAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGAACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACATACGCCGATTTGGAAAAAAtggt                                                               <  5:153242/149‑1 (MQ=255)
                                                                                      gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAAtggtg                                                              <  5:79992/149‑1 (MQ=255)
                                                                                      gTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAAtggtg                                                              <  6:238778/149‑1 (MQ=255)
                                                                                         gCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGa                                                            <  8:255324/148‑1 (MQ=255)
                                                                                           aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGacac                                                         >  3:156216/1‑149 (MQ=255)
                                                                                            ggACTCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACct                                                       >  3:222746/1‑149 (MQ=255)
                                                                                            ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAAtggtg                                                              <  2:140125/143‑1 (MQ=255)
                                                                                            ggACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAAtggtg                                                              >  1:140125/1‑143 (MQ=255)
                                                                                                  tGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGAc                                                  >  7:64347/1‑149 (MQ=255)
                                                                                                    ccAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGAc                                                  >  6:248683/1‑147 (MQ=255)
                                                                                                      agagAAATCTCAGTTTGCAATTGACTGTATATAACCGAAAAGTGAATGAGCGTAAATTTATACGAAGATTATTGGTACTGGAAGATATCTGCCCGAACTAGTGCGGACAAACTCCGATTTGGAAAAAATGCTGGACACCTCTGACGAGt                                              <  1:333352/146‑1 (MQ=255)
                                                                                                      aGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                              <  5:360015/149‑1 (MQ=255)
                                                                                                         gAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACCGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGa                                           <  1:137799/149‑1 (MQ=255)
                                                                                                          aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTTTTGGTACTGGCAGCTATCTGCCCGAACTAGTGCGGTCAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                          <  4:194710/149‑1 (MQ=255)
                                                                                                          aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                          <  1:185650/149‑1 (MQ=255)
                                                                                                          aaCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGAt                                          <  5:235646/149‑1 (MQ=255)
                                                                                                             tCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGt                                       >  6:45162/1‑149 (MQ=255)
                                                                                                               gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                              <  7:317157/140‑1 (MQ=255)
                                                                                                               gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGt                                              >  8:317157/1‑140 (MQ=255)
                                                                                                               gCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTc                                      >  8:199650/1‑148 (MQ=255)
                                                                                                                cAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTTGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAc                                    <  4:137972/149‑1 (MQ=255)
                                                                                                                cAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCAc                                    <  4:156216/149‑1 (MQ=255)
                                                                                                                   tttGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCg                                 >  6:318377/1‑149 (MQ=255)
                                                                                                                   tttGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCg                                 >  1:121137/1‑149 (MQ=255)
                                                                                                                                 tatataACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCcgc                    >  6:320989/1‑148 (MQ=255)
                                                                                                                                     taACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCg                            >  5:276124/1‑136 (MQ=255)
                                                                                                                                     taACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCg                            <  6:276124/136‑1 (MQ=255)
                                                                                                                                      aaCCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGc              >  1:398339/1‑149 (MQ=255)
                                                                                                                                          gAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTTTCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCTAACGCcaca          <  8:124292/149‑1 (MQ=255)
                                                                                                                                           aaaaGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACAt         >  3:163002/1‑149 (MQ=255)
                                                                                                                                           aaaaGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGAAGCTATCTGCCCGAGCAAGTGCGGACTAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGTTATCCGCGAACGCcacct         >  2:220184/1‑147 (MQ=255)
                                                                                                                                               gTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGc      <  2:67463/148‑1 (MQ=255)
                                                                                                                                               gTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGc      >  1:67463/1‑148 (MQ=255)
                                                                                                                                                  aCTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCcgcg  >  8:9997/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCG  >  NC_000913/1148602‑1148896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: