Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,747,161	C→A	11.5%	P11T (CCT→ACT)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,161	0	C	A	11.5%	178.2 / 6.8	69	P11T (CCT→ACT)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base A (2/7);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTG  >  NC_000913/1747014‑1747302                                                                                                                                                    |                                                                                                                                              aaTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                               <  4:300010/148‑1 (MQ=255)  aTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGcc                                                                                                                                              >  8:27289/1‑148 (MQ=255)    cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAATTAACAATGAAAAAAATTGCTATTGTGGGTGACGGGCCTAc                                                                                                                                           >  1:110397/1‑149 (MQ=255)     aaGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTAc                                                                                                                                           <  4:242814/148‑1 (MQ=255)      aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                         <  2:38999/149‑1 (MQ=255)      aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                         <  6:83039/149‑1 (MQ=255)       gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACggg                                                                                                                                        <  4:64195/149‑1 (MQ=255)                      ttttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTAtt                                                                                                                         >  8:112321/1‑149 (MQ=255)                      ttttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGAGTACAGGTAAGTAACAATGAAAATAATTGATATAGTGGGAGCCGGGCCTACGGGGATCTACACattatt                                                                                                                         >  4:94179/1‑149 (MQ=255)                        ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTTATCACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTAtt                                                                                                                         <  2:330751/147‑1 (MQ=255)                        ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTTTTTTTGTTTCCGGGACTACGGTGATCTACACCTTATtc                                                                                                                        <  4:230659/148‑1 (MQ=255)                        ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATtc                                                                                                                        <  5:247241/148‑1 (MQ=255)                            tCCATGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGAGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCt                                                                                                                   >  1:327356/1‑149 (MQ=255)                              ccTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCtt                                                                                                                  <  7:27289/148‑1 (MQ=255)                                  tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAc                                                                                                              <  3:272623/148‑1 (MQ=255)                                  tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAGCTACACCTTATTCTCGCTTCTa                                                                                                               <  3:243451/147‑1 (MQ=255)                                    tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAg                                                                                                            >  4:317995/1‑148 (MQ=255)                                         tttCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCTTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGCCCTACGGGGATCTACACCTTATTCTCGCTTCTAAATcaaca                                                                                                       >  1:216867/1‑148 (MQ=255)                                             tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                                  >  5:289785/1‑149 (MQ=255)                                             tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGAAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCATAAGCGGATCTACACGTTATACTCGCATCAACAGAAACAAACTc                                                                                                  >  6:210840/1‑149 (MQ=255)                                                aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCAc                                                                                               >  8:267/1‑149 (MQ=255)                                                       aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                    <  3:330253/121‑1 (MQ=255)                                                       aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                    >  4:330253/1‑121 (MQ=255)                                                       aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                                        <  7:267/149‑1 (MQ=255)                                                         aaTCTCAACCAAACATATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctattt                                                                                      <  6:251798/149‑1 (MQ=255)                                                                aCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCGATCtt                                                                               >  2:231884/1‑149 (MQ=255)                                                                 ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                              >  7:355063/1‑149 (MQ=255)                                                                  aaatcTTATCTGATAAGAGTAAATTCGATAGAATGCGTACAGGTACGTAACAATGAAAAAAATTGATATTGTGGGTGCCGTGCCTACTGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCg                                                                             <  5:210840/145‑1 (MQ=255)                                                                    aaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAg                                                                           <  7:112321/149‑1 (MQ=255)                                                                     aCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGACTAAGGGGATCTACACATTATTATAGCTTCTACAGCAAAAAACTCCACTTTATATTTCTATATTCTAg                                                                           >  5:57835/1‑148 (MQ=255)                                                                       ttATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                        >  3:214706/1‑149 (MQ=255)                                                                       ttATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGACTACGGGGATCTACACCTTATTATCGATTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                        >  1:295908/1‑149 (MQ=255)                                                                          tCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCt                                                                     >  2:180952/1‑149 (MQ=255)                                                                             gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  7:299494/1‑149 (MQ=255)                                                                              aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGTTCTACACCTAATAATCGCTTCTACAGCAACAATCTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  1:88982/1‑148 (MQ=255)                                                                               tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  2:134953/1‑147 (MQ=255)                                                                                 acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACATTGAAAAATTTTTTTATTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAgg                                                              <  2:216867/149‑1 (MQ=255)                                                                                  cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  6:289785/149‑1 (MQ=255)                                                                                  cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTGTACAGCAACAAACTCCAGTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  3:317995/149‑1 (MQ=255)                                                                                  cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             >  1:335542/1‑149 (MQ=255)                                                                                  cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  8:299494/149‑1 (MQ=255)                                                                                   acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                            >  7:365683/1‑149 (MQ=255)                                                                                   acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                            >  6:75785/1‑149 (MQ=255)                                                                                    cTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCg                                                           <  4:214706/149‑1 (MQ=255)                                                                                     tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGa                                                                            <  1:379612/131‑1 (MQ=255)                                                                                     tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGa                                                                            >  2:379612/1‑131 (MQ=255)                                                                                       aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTGGTTTCCGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgt                                                         <  2:327356/148‑1 (MQ=255)                                                                                       aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtg                                                        >  7:386919/1‑149 (MQ=255)                                                                                       aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgt                                                         <  5:75785/148‑1 (MQ=255)                                                                                            gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                    >  8:188805/1‑148 (MQ=255)                                                                                              aaGAATGAGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGGTTCGGGGCCTACTGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTAGGGAt                                                 <  6:57835/149‑1 (MQ=255)                                                                                                gAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTATTTTTGTTGCCGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGc                                               <  8:329818/149‑1 (MQ=255)                                                                                                       tACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCTAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATAAAg                                        >  5:227292/1‑149 (MQ=255)                                                                                                               agtaaCCATGAAAATATTTGCTTTTGTGGTGGCGGGGCCTACGGTGAGATACTTCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                 <  2:88982/148‑1 (MQ=255)                                                                                                               agtaaCAATGAAATAAATTGCTATTGTGTGTGCCGGGCCTAGGGGCATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                 <  3:94179/148‑1 (MQ=255)                                                                                                               agtaaCAATGAAAAATATTTTTTTTTTGGTTTCGGGGACTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAgg                                <  6:113350/149‑1 (MQ=255)                                                                                                               agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGACGAg                                 <  7:188805/148‑1 (MQ=255)                                                                                                                 taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGa                               >  6:379669/1‑148 (MQ=255)                                                                                                                   aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                            >  2:215430/1‑149 (MQ=255)                                                                                                                   aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTATCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaa                             <  5:74048/148‑1 (MQ=255)                                                                                                                    cAATGAAAAAATTTGGTTTTGTGTGTGCCGGGCCTACGGTGATCTACAGCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAAc                           <  2:295908/149‑1 (MQ=255)                                                                                                                     aaTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACt                          >  8:336722/1‑149 (MQ=255)                                                                                                                       tGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTc                         >  1:311411/1‑148 (MQ=255)                                                                                                                        gAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCAGACAGTGATGAGGAACACTCa                        >  4:235952/1‑148 (MQ=255)                                                                                                                           aaaaaTTGATATTGAGGCTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaaa                    <  8:386919/149‑1 (MQ=255)                                                                                                                              taTTGATATTTTGGGTGCGGTGCCGACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                 <  2:320982/148‑1 (MQ=255)                                                                                                                              aaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                 >  4:36706/1‑149 (MQ=255)                                                                                                                                ttGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatgatg               <  1:134953/149‑1 (MQ=255)                                                                                                                                    tATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTgg           >  6:310129/1‑149 (MQ=255)                                                                                                                                      ttGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGc          >  7:161500/1‑148 (MQ=255)                                                                                                                                             tGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTg  <  1:180952/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              AATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTG  >  NC_000913/1747014‑1747302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑