Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,747,161 C→A 11.5% P11T (CCT→ACT)  ydhS → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,747,1610CA11.5% 178.2 / 6.8 69P11T (CCT→ACT) ydhSputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (32/28);  new base A (2/7);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTG  >  NC_000913/1747014‑1747302
                                                                                                                                                   |                                                                                                                                             
aaTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                               <  4:300010/148‑1 (MQ=255)
 aTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGcc                                                                                                                                              >  8:27289/1‑148 (MQ=255)
   cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAATTAACAATGAAAAAAATTGCTATTGTGGGTGACGGGCCTAc                                                                                                                                           >  1:110397/1‑149 (MQ=255)
    aaGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTAc                                                                                                                                           <  4:242814/148‑1 (MQ=255)
     aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                         <  2:38999/149‑1 (MQ=255)
     aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                         <  6:83039/149‑1 (MQ=255)
      gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACggg                                                                                                                                        <  4:64195/149‑1 (MQ=255)
                     ttttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTAtt                                                                                                                         >  8:112321/1‑149 (MQ=255)
                     ttttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGAGTACAGGTAAGTAACAATGAAAATAATTGATATAGTGGGAGCCGGGCCTACGGGGATCTACACattatt                                                                                                                         >  4:94179/1‑149 (MQ=255)
                       ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTTATCACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTAtt                                                                                                                         <  2:330751/147‑1 (MQ=255)
                       ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTTTTTTTGTTTCCGGGACTACGGTGATCTACACCTTATtc                                                                                                                        <  4:230659/148‑1 (MQ=255)
                       ttCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATtc                                                                                                                        <  5:247241/148‑1 (MQ=255)
                           tCCATGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGAGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCt                                                                                                                   >  1:327356/1‑149 (MQ=255)
                             ccTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCtt                                                                                                                  <  7:27289/148‑1 (MQ=255)
                                 tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTAc                                                                                                              <  3:272623/148‑1 (MQ=255)
                                 tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAGCTACACCTTATTCTCGCTTCTa                                                                                                               <  3:243451/147‑1 (MQ=255)
                                   tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAg                                                                                                            >  4:317995/1‑148 (MQ=255)
                                        tttCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCTTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGCCCTACGGGGATCTACACCTTATTCTCGCTTCTAAATcaaca                                                                                                       >  1:216867/1‑148 (MQ=255)
                                            tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                                  >  5:289785/1‑149 (MQ=255)
                                            tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGAAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCATAAGCGGATCTACACGTTATACTCGCATCAACAGAAACAAACTc                                                                                                  >  6:210840/1‑149 (MQ=255)
                                               aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCAc                                                                                               >  8:267/1‑149 (MQ=255)
                                                      aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                    <  3:330253/121‑1 (MQ=255)
                                                      aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGc                                                                                                                    >  4:330253/1‑121 (MQ=255)
                                                      aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                                        <  7:267/149‑1 (MQ=255)
                                                        aaTCTCAACCAAACATATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctattt                                                                                      <  6:251798/149‑1 (MQ=255)
                                                               aCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCGATCtt                                                                               >  2:231884/1‑149 (MQ=255)
                                                                ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                              >  7:355063/1‑149 (MQ=255)
                                                                 aaatcTTATCTGATAAGAGTAAATTCGATAGAATGCGTACAGGTACGTAACAATGAAAAAAATTGATATTGTGGGTGCCGTGCCTACTGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCg                                                                             <  5:210840/145‑1 (MQ=255)
                                                                   aaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAg                                                                           <  7:112321/149‑1 (MQ=255)
                                                                    aCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGACTAAGGGGATCTACACATTATTATAGCTTCTACAGCAAAAAACTCCACTTTATATTTCTATATTCTAg                                                                           >  5:57835/1‑148 (MQ=255)
                                                                      ttATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                        >  3:214706/1‑149 (MQ=255)
                                                                      ttATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGACTACGGGGATCTACACCTTATTATCGATTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAg                                                                        >  1:295908/1‑149 (MQ=255)
                                                                         tCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCt                                                                     >  2:180952/1‑149 (MQ=255)
                                                                            gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  7:299494/1‑149 (MQ=255)
                                                                             aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGTTCTACACCTAATAATCGCTTCTACAGCAACAATCTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  1:88982/1‑148 (MQ=255)
                                                                              tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                                  >  2:134953/1‑147 (MQ=255)
                                                                                acacTAAATTCGAAAGAATGCGTACAGGTAAGTAACATTGAAAAATTTTTTTATTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAgg                                                              <  2:216867/149‑1 (MQ=255)
                                                                                 cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTTCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  6:289785/149‑1 (MQ=255)
                                                                                 cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTGTACAGCAACAAACTCCAGTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  3:317995/149‑1 (MQ=255)
                                                                                 cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             >  1:335542/1‑149 (MQ=255)
                                                                                 cacTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGc                                                             <  8:299494/149‑1 (MQ=255)
                                                                                  acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                            >  7:365683/1‑149 (MQ=255)
                                                                                  acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                            >  6:75785/1‑149 (MQ=255)
                                                                                   cTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCg                                                           <  4:214706/149‑1 (MQ=255)
                                                                                    tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGa                                                                            <  1:379612/131‑1 (MQ=255)
                                                                                    tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGa                                                                            >  2:379612/1‑131 (MQ=255)
                                                                                      aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTGGTTTCCGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgt                                                         <  2:327356/148‑1 (MQ=255)
                                                                                      aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtg                                                        >  7:386919/1‑149 (MQ=255)
                                                                                      aaTTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgt                                                         <  5:75785/148‑1 (MQ=255)
                                                                                           gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCgg                                                    >  8:188805/1‑148 (MQ=255)
                                                                                             aaGAATGAGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGGTTCGGGGCCTACTGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTAGGGAt                                                 <  6:57835/149‑1 (MQ=255)
                                                                                               gAATGCGTACAGGTAAGTAACAATGAAAAAAATTTTTATTTTTGTTGCCGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGc                                               <  8:329818/149‑1 (MQ=255)
                                                                                                      tACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCTAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATAAAg                                        >  5:227292/1‑149 (MQ=255)
                                                                                                              agtaaCCATGAAAATATTTGCTTTTGTGGTGGCGGGGCCTACGGTGAGATACTTCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                 <  2:88982/148‑1 (MQ=255)
                                                                                                              agtaaCAATGAAATAAATTGCTATTGTGTGTGCCGGGCCTAGGGGCATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                                 <  3:94179/148‑1 (MQ=255)
                                                                                                              agtaaCAATGAAAAATATTTTTTTTTTGGTTTCGGGGACTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAgg                                <  6:113350/149‑1 (MQ=255)
                                                                                                              agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGACGAg                                 <  7:188805/148‑1 (MQ=255)
                                                                                                                taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGa                               >  6:379669/1‑148 (MQ=255)
                                                                                                                  aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                            >  2:215430/1‑149 (MQ=255)
                                                                                                                  aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTATCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaa                             <  5:74048/148‑1 (MQ=255)
                                                                                                                   cAATGAAAAAATTTGGTTTTGTGTGTGCCGGGCCTACGGTGATCTACAGCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAAc                           <  2:295908/149‑1 (MQ=255)
                                                                                                                    aaTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACt                          >  8:336722/1‑149 (MQ=255)
                                                                                                                      tGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTc                         >  1:311411/1‑148 (MQ=255)
                                                                                                                       gAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCAGACAGTGATGAGGAACACTCa                        >  4:235952/1‑148 (MQ=255)
                                                                                                                          aaaaaTTGATATTGAGGCTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaaa                    <  8:386919/149‑1 (MQ=255)
                                                                                                                             taTTGATATTTTGGGTGCGGTGCCGACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                 <  2:320982/148‑1 (MQ=255)
                                                                                                                             aaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatga                 >  4:36706/1‑149 (MQ=255)
                                                                                                                               ttGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatgatg               <  1:134953/149‑1 (MQ=255)
                                                                                                                                   tATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTgg           >  6:310129/1‑149 (MQ=255)
                                                                                                                                     ttGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGc          >  7:161500/1‑148 (MQ=255)
                                                                                                                                            tGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTg  <  1:180952/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
AATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGGCAAATATTG  >  NC_000913/1747014‑1747302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: