Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,147,351 T→A 5.1% S64C (AGT→TGT)  alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,147,3510TA 5.1% 245.2 / 2.5 79S64C (AGT→TGT) alkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base A (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

ACAGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACA  >  NC_000913/2147211‑2147497
                                                                                                                                            |                                                                                                                                                  
acaGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTa                                                                                                                                            >  7:54888/1‑149 (MQ=255)
 caGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTCCGGCAACAGGTTCTAAACCTGCACTTAAATTTAt                                                                                                                                           <  7:223711/149‑1 (MQ=255)
    ccGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTAtgtg                                                                                                                                        <  8:322121/149‑1 (MQ=255)
      gtgTAAACGCAATCCGGGCCGCGCCTCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCAGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                      <  8:254230/147‑1 (MQ=255)
      gggTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                      <  1:195095/149‑1 (MQ=255)
      gggTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                      <  8:254234/149‑1 (MQ=255)
        gTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCaga                                                                                                                                    <  5:296666/149‑1 (MQ=255)
           caCGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACATGCGGCTCATTTTCGCCAGACACTCTGCGGGAACAGGTTCTAAACCTGCACATAAATTTAAGTGCaggtt                                                                                                                                  <  2:154916/147‑4 (MQ=255)
           aaCGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACGCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTa                                                                                                                                 >  7:304869/1‑149 (MQ=255)
              gCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATgg                                                                                                                              >  3:145213/1‑149 (MQ=255)
              gCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATgg                                                                                                                              >  1:343217/1‑149 (MQ=255)
                  tCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCggg                                                                                                                          <  4:32947/149‑1 (MQ=255)
                    cGGGCCGCTCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGATCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                         <  5:125209/148‑1 (MQ=255)
                     gggCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCta                                                                                                                       >  3:304470/1‑149 (MQ=255)
                          gcgccgcgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                         <  4:201698/142‑1 (MQ=255)
                          gcgccgcgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                         >  3:201698/1‑142 (MQ=255)
                                cgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                            <  2:139299/149‑1 (MQ=255)
                                        cTGCCCCACGCTCCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcac                                                                                                    <  6:239153/149‑1 (MQ=255)
                                        cTGCCCAAAGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcac                                                                                                    >  5:239153/1‑149 (MQ=255)
                                         tGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcacc                                                                                                   >  4:129473/1‑149 (MQ=255)
                                             cAACGCACCGTTAGCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                            <  7:128954/136‑1 (MQ=255)
                                             cAACGCACCGTTAGCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                            >  8:128954/1‑136 (MQ=255)
                                              aaCGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCAcgcc                                                                                              <  8:54888/149‑1 (MQ=255)
                                              aaCGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCAcgcc                                                                                              >  6:177329/1‑149 (MQ=255)
                                                   aCCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGat                                                                                         >  2:8068/1‑149 (MQ=255)
                                                      gTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATAtt                                                                                      >  5:146062/1‑149 (MQ=255)
                                                          aCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTTAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGcc                                                                                  <  2:196976/149‑1 (MQ=255)
                                                          aCAATTTGTGGCTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAAATTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGcc                                                                                  >  2:392032/1‑149 (MQ=255)
                                                           cAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGccc                                                                                 >  4:85902/1‑149 (MQ=255)
                                                                    ggTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACGCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                        <  8:304869/149‑1 (MQ=255)
                                                                     ggtACATTGCAGAACAACCAGGCGGCCCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGAc                                                                       <  6:235973/147‑1 (MQ=255)
                                                                          aTTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCGGCACTTAAATTTATGTGCAGGGTAGGGCGGGCGAGCTGCGGAATAGCAGTCCCCACGGCGCGATATTCGCCCCCCGCCAGACTACgg                                                                  >  4:14826/1‑149 (MQ=255)
                                                                           ttGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                        >  2:295595/1‑142 (MQ=255)
                                                                           ttGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                        <  1:295595/142‑1 (MQ=255)
                                                                              cAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCa                                                               >  7:248516/1‑148 (MQ=255)
                                                                                gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGTCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCCCCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                            >  4:113474/1‑149 (MQ=255)
                                                                                gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                            <  5:85094/149‑1 (MQ=255)
                                                                                gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                            >  6:85094/1‑149 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAg                                                                                                           >  5:227558/1‑101 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAg                                                                                                           <  6:227558/101‑1 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                >  1:194543/1‑144 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                >  1:372159/1‑144 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                <  2:194543/144‑1 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                <  2:372159/144‑1 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataat                                                           <  1:8068/149‑1 (MQ=255)
                                                                                 aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                            >  2:32988/1‑148 (MQ=255)
                                                                                    aaaCAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGAGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGCCATAAAAAc                                                        >  8:337733/1‑149 (MQ=255)
                                                                                     aaCAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACt                                                       <  3:129473/149‑1 (MQ=255)
                                                                                          gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCg                                                                                    >  4:32581/1‑115 (MQ=255)
                                                                                          gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCg                                                                                    <  3:32581/115‑1 (MQ=255)
                                                                                          gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCAtaataa                                                         <  4:147324/142‑1 (MQ=255)
                                                                                          gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCAtaataa                                                         >  3:147324/1‑142 (MQ=255)
                                                                                             gcTCATTATCGCCAGACACTCTGCGGCAACAGGGTCTAAACCTGCACTTAAATTTATGTGCAGAGGATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGa                                               <  2:391730/149‑1 (MQ=255)
                                                                                               tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCa                                                                          >  5:35327/1‑120 (MQ=255)
                                                                                               tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCa                                                                          <  6:35327/120‑1 (MQ=255)
                                                                                               tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACAGCCAGACTACGGGCATAATAACTGTCCGCGAcc                                             >  1:162896/1‑149 (MQ=255)
                                                                                                  ttttCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGtt                                          <  5:177329/149‑1 (MQ=255)
                                                                                                      cGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTcc                                       <  3:85902/148‑1 (MQ=255)
                                                                                                           gACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTg                                 <  4:145213/149‑1 (MQ=255)
                                                                                                                tctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCTCCCACCGCCAGACTACGGGCATAAAAAATGTCCGCGACCGTTTCCACACTGCTCAc                            >  4:221645/1‑149 (MQ=255)
                                                                                                                tctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCAc                            >  7:367847/1‑149 (MQ=255)
                                                                                                                    cGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCAcc                           <  4:304470/146‑1 (MQ=255)
                                                                                                                    cGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCa                        >  5:18128/1‑149 (MQ=255)
                                                                                                                      gCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCATAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACg                      >  3:1805/1‑149 (MQ=255)
                                                                                                                         aCAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACgg                                                                  <  8:294742/102‑1 (MQ=255)
                                                                                                                         aCAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACgg                                                                  >  7:294742/1‑102 (MQ=255)
                                                                                                                            ggTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                <  5:231365/149‑1 (MQ=255)
                                                                                                                              ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCga              <  1:392032/149‑1 (MQ=255)
                                                                                                                               tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                >  4:171505/1‑146 (MQ=255)
                                                                                                                               ccatactcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACAGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                >  8:189981/8‑146 (MQ=255)
                                                                                                                                catactcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCgag             >  4:309005/7‑148 (MQ=255)
                                                                                                                                    ctcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt         <  3:309005/146‑1 (MQ=255)
                                                                                                                                     ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTc                                        <  2:224928/116‑1 (MQ=255)
                                                                                                                                     ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTc                                        >  1:224928/1‑116 (MQ=255)
                                                                                                                                     ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc       >  4:79966/1‑149 (MQ=255)
                                                                                                                                      cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc      <  8:248516/149‑1 (MQ=255)
                                                                                                                                      cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc       >  6:313042/1‑148 (MQ=255)
                                                                                                                                       tGCACTTAAATTTATGTGCACAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATACGCCCACCGCCAGACTACGGGAATAATAACAGTCCGCGACCGTATCCACACTGCTCACCGCACGGGCGTCGAGAAATccc      >  6:285360/1‑148 (MQ=255)
                                                                                                                                          aCTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACa  <  3:14826/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
ACAGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACA  >  NC_000913/2147211‑2147497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: