Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,147,360 T→A 5.3% I61L (ATA→TTA)  alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,147,3600TA 5.3% 242.2 / 3.3 76I61L (ATA→TTA) alkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base T (37/35);  new base A (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

CAGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAG  >  NC_000913/2147212‑2147508
                                                                                                                                                    |                                                                                                                                                    
caGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTCCGGCAACAGGTTCTAAACCTGCACTTAAATTTAt                                                                                                                                                      <  7:223711/149‑1 (MQ=255)
   ccGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTAtgtg                                                                                                                                                   <  8:322121/149‑1 (MQ=255)
     gtgTAAACGCAATCCGGGCCGCGCCTCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCAGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                                 <  8:254230/147‑1 (MQ=255)
     gggTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                                 <  1:195095/149‑1 (MQ=255)
     gggTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCa                                                                                                                                                 <  8:254234/149‑1 (MQ=255)
       gTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCaga                                                                                                                                               <  5:296666/149‑1 (MQ=255)
          caCGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACATGCGGCTCATTTTCGCCAGACACTCTGCGGGAACAGGTTCTAAACCTGCACATAAATTTAAGTGCaggtt                                                                                                                                             <  2:154916/147‑4 (MQ=255)
          aaCGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACGCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTa                                                                                                                                            >  7:304869/1‑149 (MQ=255)
             gCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATgg                                                                                                                                         >  1:343217/1‑149 (MQ=255)
             gCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATgg                                                                                                                                         >  3:145213/1‑149 (MQ=255)
                 tCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCggg                                                                                                                                     <  4:32947/149‑1 (MQ=255)
                   cGGGCCGCTCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGATCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                                    <  5:125209/148‑1 (MQ=255)
                    gggCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCta                                                                                                                                  >  3:304470/1‑149 (MQ=255)
                         gcgccgcgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                                    <  4:201698/142‑1 (MQ=255)
                         gcgccgcgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGc                                                                                                                                    >  3:201698/1‑142 (MQ=255)
                               cgccTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                                       <  2:139299/149‑1 (MQ=255)
                                       cTGCCCCACGCTCCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcac                                                                                                               <  6:239153/149‑1 (MQ=255)
                                       cTGCCCAAAGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcac                                                                                                               >  5:239153/1‑149 (MQ=255)
                                        tGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTcacc                                                                                                              >  4:129473/1‑149 (MQ=255)
                                            cAACGCACCGTTAGCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                                       <  7:128954/136‑1 (MQ=255)
                                            cAACGCACCGTTAGCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATa                                                                                                                       >  8:128954/1‑136 (MQ=255)
                                             aaCGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCAcgcc                                                                                                         >  6:177329/1‑149 (MQ=255)
                                             aaCGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCAcgcc                                                                                                         <  8:54888/149‑1 (MQ=255)
                                                  aCCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGat                                                                                                    >  2:8068/1‑149 (MQ=255)
                                                     gTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATAtt                                                                                                 >  5:146062/1‑149 (MQ=255)
                                                         aCAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTTAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGcc                                                                                             <  2:196976/149‑1 (MQ=255)
                                                         aCAATTTGTGGCTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAAATTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGcc                                                                                             >  2:392032/1‑149 (MQ=255)
                                                          cAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGccc                                                                                            >  4:85902/1‑149 (MQ=255)
                                                                   ggTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACGCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                                   <  8:304869/149‑1 (MQ=255)
                                                                    ggtACATTGCAGAACAACCAGGCGGCCCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGAc                                                                                  <  6:235973/147‑1 (MQ=255)
                                                                         aTTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCGGCACTTAAATTTATGTGCAGGGTAGGGCGGGCGAGCTGCGGAATAGCAGTCCCCACGGCGCGATATTCGCCCCCCGCCAGACTACgg                                                                             >  4:14826/1‑149 (MQ=255)
                                                                          ttGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                                   >  2:295595/1‑142 (MQ=255)
                                                                          ttGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGa                                                                                   <  1:295595/142‑1 (MQ=255)
                                                                             cAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCa                                                                          >  7:248516/1‑148 (MQ=255)
                                                                               gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGTCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCCCCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                                       >  4:113474/1‑149 (MQ=255)
                                                                               gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                                       >  6:85094/1‑149 (MQ=255)
                                                                               gATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                                       <  5:85094/149‑1 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAg                                                                                                                      <  6:227558/101‑1 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAg                                                                                                                      >  5:227558/1‑101 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                           >  1:194543/1‑144 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                           >  1:372159/1‑144 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                           <  2:194543/144‑1 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGc                                                                           <  2:372159/144‑1 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataat                                                                      <  1:8068/149‑1 (MQ=255)
                                                                                aTCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCataa                                                                       >  2:32988/1‑148 (MQ=255)
                                                                                   aaaCAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGAGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGCCATAAAAAc                                                                   >  8:337733/1‑149 (MQ=255)
                                                                                    aaCAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACt                                                                  <  3:129473/149‑1 (MQ=255)
                                                                                         gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCg                                                                                               >  4:32581/1‑115 (MQ=255)
                                                                                         gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCg                                                                                               <  3:32581/115‑1 (MQ=255)
                                                                                         gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCAtaataa                                                                    <  4:147324/142‑1 (MQ=255)
                                                                                         gcggcTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCAtaataa                                                                    >  3:147324/1‑142 (MQ=255)
                                                                                            gcTCATTATCGCCAGACACTCTGCGGCAACAGGGTCTAAACCTGCACTTAAATTTATGTGCAGAGGATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGa                                                          <  2:391730/149‑1 (MQ=255)
                                                                                              tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCa                                                                                     >  5:35327/1‑120 (MQ=255)
                                                                                              tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCa                                                                                     <  6:35327/120‑1 (MQ=255)
                                                                                              tCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACAGCCAGACTACGGGCATAATAACTGTCCGCGAcc                                                        >  1:162896/1‑149 (MQ=255)
                                                                                                 ttttCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGtt                                                     <  5:177329/149‑1 (MQ=255)
                                                                                                     cGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTcc                                                  <  3:85902/148‑1 (MQ=255)
                                                                                                          gACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTg                                            <  4:145213/149‑1 (MQ=255)
                                                                                                               tctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCTCCCACCGCCAGACTACGGGCATAAAAAATGTCCGCGACCGTTTCCACACTGCTCAc                                       >  4:221645/1‑149 (MQ=255)
                                                                                                               tctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCAc                                       >  7:367847/1‑149 (MQ=255)
                                                                                                                   cGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCAcc                                      <  4:304470/146‑1 (MQ=255)
                                                                                                                   cGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCa                                   >  5:18128/1‑149 (MQ=255)
                                                                                                                     gCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCATAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACg                                 >  3:1805/1‑149 (MQ=255)
                                                                                                                        aCAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACgg                                                                             <  8:294742/102‑1 (MQ=255)
                                                                                                                        aCAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACgg                                                                             >  7:294742/1‑102 (MQ=255)
                                                                                                                           ggTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                           <  5:231365/149‑1 (MQ=255)
                                                                                                                             ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCga                         <  1:392032/149‑1 (MQ=255)
                                                                                                                              tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                           >  4:171505/1‑146 (MQ=255)
                                                                                                                              ccatactcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACAGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGggcggc                           >  8:189981/8‑146 (MQ=255)
                                                                                                                               catactcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCgag                        >  4:309005/7‑148 (MQ=255)
                                                                                                                                   ctcTGCACATAAATTTAAGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt                    <  3:309005/146‑1 (MQ=255)
                                                                                                                                    ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTc                                                   <  2:224928/116‑1 (MQ=255)
                                                                                                                                    ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTc                                                   >  1:224928/1‑116 (MQ=255)
                                                                                                                                    ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc                  >  4:79966/1‑149 (MQ=255)
                                                                                                                                     cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc                 <  8:248516/149‑1 (MQ=255)
                                                                                                                                     cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc                  >  6:313042/1‑148 (MQ=255)
                                                                                                                                      tGCACTTAAATTTATGTGCACAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATACGCCCACCGCCAGACTACGGGAATAATAACAGTCCGCGACCGTATCCACACTGCTCACCGCACGGGCGTCGAGAAATccc                 >  6:285360/1‑148 (MQ=255)
                                                                                                                                         aCTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACa             <  3:14826/149‑1 (MQ=255)
                                                                                                                                                    tgtgCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAACCCCAACATCCACGACCAg  <  5:313042/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGCCGGGTAAACGCAATCCGGGCCGCGCCGCGCCTAACCTGCCCAACGCACCGTTAACAATTTGTGGGTTACATTGCAGATCAAACAGGCGGCTCATTTTCGCCAGACACTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAG  >  NC_000913/2147212‑2147508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑