| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,847,034 | C→T | 5.1% | A127T (GCG→ACG) | hycD ← | hydrogenase 3, membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,847,034 | 0 | C | T | 5.1% | 253.7 / 2.9 | 79 | A127T (GCG→ACG) | hycD | hydrogenase 3, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base C (40/34); new base T (2/2); total (43/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
CCAGCGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGA > NC_000913/2846887‑2847180 | ccAGCGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTcgc < 4:128426/148‑1 (MQ=255)ccAGCGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTcgc < 1:12234/148‑1 (MQ=255) cAGCGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCgcgc < 2:65232/149‑1 (MQ=255) cGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGa > 6:350864/1‑149 (MQ=255) cGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGa < 3:366916/149‑1 (MQ=255) cGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGa > 8:150149/1‑149 (MQ=255) cGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGa > 2:149927/1‑149 (MQ=255) gATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAg > 5:224898/1‑149 (MQ=255) gCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCgg > 1:163454/1‑149 (MQ=255) ctctGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGGCCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGt < 4:43205/149‑1 (MQ=255) ctctGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCgg > 8:365129/1‑148 (MQ=255) tctGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGt > 3:346532/1‑148 (MQ=255) gCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACg < 2:357666/149‑1 (MQ=255) aGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACggg < 8:295422/147‑1 (MQ=255) aGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACggg < 6:224898/147‑1 (MQ=255) cGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTAc > 5:4283/1‑149 (MQ=255) gCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTTGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCg < 2:163454/149‑1 (MQ=255) gTGATAAACGGTGTCGTTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTAt < 3:29008/149‑1 (MQ=255) gTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTAt > 4:109171/1‑149 (MQ=255) tGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATc < 3:244933/149‑1 (MQ=255) tGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATc < 8:326433/149‑1 (MQ=255) aTAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCCCAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCCAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGGATc > 2:245819/1‑147 (MQ=255) aTAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATcca < 8:264614/149‑1 (MQ=255) aTAAACGGTGTCGGGGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATcca < 2:242097/149‑1 (MQ=255) aaaCGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATccaga > 2:87496/1‑149 (MQ=255) aaCGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCACCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCgg > 2:42260/1‑140 (MQ=255) aaCGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCACCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCgg < 1:42260/140‑1 (MQ=255) tgtCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGaa < 1:76941/149‑1 (MQ=255) ggTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATgg > 1:75403/1‑149 (MQ=255) ggTGATGTTGCTCATGTTGGTGGAACCGGCACCCTGTGCGCCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATg > 6:316926/1‑148 (MQ=255) gATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCa > 3:318854/1‑148 (MQ=255) aTGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCaaa < 2:75403/149‑1 (MQ=255) aTGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCa > 8:143122/1‑147 (MQ=255) tggtGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAaag < 1:387516/146‑1 (MQ=255) tGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAAcgc > 2:143248/1‑149 (MQ=255) gTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAAcgcg > 2:197563/1‑149 (MQ=255) gtggAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGa < 3:323160/148‑1 (MQ=255) tggAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATg < 7:150149/149‑1 (MQ=255) gAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGc < 1:143248/149‑1 (MQ=255) aaCCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGCCCAGAAATGGCAAAGAAGAGACGCGCGATGGc > 4:248508/1‑148 (MQ=255) aaCCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGc > 3:19545/1‑148 (MQ=255) aCCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGGCAAGCATCGCGTCACGGCTCGCGCCGATCGCGGGAAACGGGCGACCGGTAGCCAGCCCAGAAAGGGCAAAGAAGACACGCGCGATGGCa > 4:54186/1‑148 (MQ=255) aCCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCaa < 5:350864/149‑1 (MQ=255) ccGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCaaa < 5:136932/149‑1 (MQ=255) ccGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCa > 6:136932/1‑147 (MQ=255) aaCCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAgaga > 3:12282/1‑148 (MQ=255) aaCCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGAt < 7:143122/149‑1 (MQ=255) cGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAg < 4:19545/149‑1 (MQ=255) cGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAg < 1:24093/149‑1 (MQ=255) cGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAg > 5:356099/1‑149 (MQ=255) aaCCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGa < 2:384320/149‑1 (MQ=255) aaCCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGAGCCGCTAGCGGTAAACGTGCTACCGGTATCCAGACCACAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAg > 5:163510/1‑145 (MQ=255) acaGACCAAGCAGCAGCATCGGTTCGACCAGTACGCCAAGCATCGCTTCACGGCTCGTGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCaaa > 3:240067/1‑149 (MQ=255) acaGACCAAGCAGCAGCATCGGTTCGACCAGTACGCCAAGCATCGCTTCACGGCTCGTGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCaa > 7:363143/1‑148 (MQ=255) caGACCAAGCAGCAGCATCGGTTCGACCAGTACGCCAAGCATCGCTTCACGGCTCGTGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAAt < 4:240067/149‑1 (MQ=255) caGACCAAGCAGCAGCATCGGTTCGACCAGTACGCCAAGCATCGCTTCACGGCTCGTGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAAt < 8:363143/149‑1 (MQ=255) aGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATc < 7:365129/149‑1 (MQ=255) cAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCAcc > 5:186340/1‑148 (MQ=255) aagaagCAGCATCGGTTCGACCATCAACCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACAACAAATGGCAAAGAAGCAACGCGCGATCGCAAAGACATACAGTAAGGTGATCaaa > 4:157612/1‑142 (MQ=255) aaGCAGCAGCATCGTTTCGACCAGCACGCCAATCATCGCTTCAATTATCGCGCCGATAGCGGTAAACTGGCTAACGTTATCCAGACCAGAAATGGCTAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCaaa < 3:157612/142‑1 (MQ=255) gcagcagcaTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGt > 1:24009/1‑149 (MQ=255) gcagcaTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATc > 5:176884/1‑134 (MQ=255) gcagcaTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATc < 6:176884/134‑1 (MQ=255) gcagcaTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGc > 8:102621/1‑149 (MQ=255) gcaTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCgg > 6:48115/1‑148 (MQ=255) aTCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAg > 2:268330/1‑149 (MQ=255) gTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCg > 1:87136/1‑147 (MQ=255) gTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGga < 3:109171/149‑1 (MQ=255) ttCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGgag < 4:346532/149‑1 (MQ=255) cGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGaa > 1:191235/1‑149 (MQ=255) gACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGaa < 7:70191/148‑1 (MQ=255) gACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGaa < 1:197563/148‑1 (MQ=255) gACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAAc < 6:186340/149‑1 (MQ=255) cAGCACGCCAAGCATCGCTTCACGGCTCGCTCCGATAGCGGTAAACGGGCTACCGGTATCCTGACCAGAAATGTCAAAGAACAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGa < 5:250818/149‑1 (MQ=255) aGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGCGCTACCGGTATCCAGACCAGAAATCGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCTAc > 5:141781/1‑149 (MQ=255) gCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTca < 1:87496/149‑1 (MQ=255) cAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGCAATGGCAAAGAAGACACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGACCCGACGGTcccc > 6:7105/1‑149 (MQ=255) cAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCACATCACCCACTTGCGGCAGCGGAGAACCGACGGTcacc > 6:375459/1‑149 (MQ=255) cAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTcacc > 6:170056/1‑149 (MQ=255) cAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTcac > 8:96842/1‑148 (MQ=255) aTCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCgg > 2:352509/1‑149 (MQ=255) cTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGc > 2:81190/1‑149 (MQ=255) gcgcCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGa > 3:226955/1‑148 (MQ=255) gcgcCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGa < 5:141694/148‑1 (MQ=255) | CCAGCGGGATGCTCTGGCTCAGCGGCCAGTGATAAACGGTGTCGGTGATGTTGCTGATGTTGGTGGAACCGGCAACCTGTGCGGCAACCCACAGACCAAGCAGCAGCATCGGTTCGACCAGCACGCCAAGCATCGCTTCACGGCTCGCGCCGATAGCGGTAAACGGGCTACCGGTATCCAGACCAGAAATGGCAAAGAAGAAACGCGCGATGGCAAAGAGATACAGTAAGGTGATCAAATCACCCAGTTGCGGCAGCGGAGAACCGACGGTCACCACCGGCAGCGCAGTAGCGA > NC_000913/2846887‑2847180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |