| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,947,466 | T→C | 19.3% | intergenic (+3/‑31) | metZ → / → metW | tRNA‑Met/tRNA‑Met |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,947,466 | 0 | T | C | 19.3% | 38.1 / 4.3 | 27 | intergenic (+3/‑31) | metZ/metW | tRNA‑Met/tRNA‑Met |
| Reads supporting (aligned to +/- strand): ref base T (6/13); new base C (2/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACG > NC_000913/2947319‑2947602 | aaaTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCATCCAAtt > 1:168229/1‑148 (MQ=255) ccGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt < 7:190241/149‑1 (MQ=255) cGTGATGGGGGTAAAAACTAGGGTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCTCGGACGCTGGGTGGAGCAGCCTGTTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCAACCAATTAAAAtt < 2:236745/149‑1 (MQ=21) ttGATCGGGGTAATAAATAGGTTGCATGATAACGCGAGGTGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt < 4:71142/148‑1 (MQ=21) aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat < 8:54046/149‑1 (MQ=255) aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat < 8:189911/149‑1 (MQ=255) ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGaa < 2:236910/149‑1 (MQ=255) gggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTT‑ATGAAGt > 6:289443/1‑149 (MQ=255) aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCa < 7:200882/149‑1 (MQ=255) aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGCAGCAGCCTGGTAGCTCGTCGGGCTCATAACCAGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TAAAATTTGAATAATTaaaag > 8:351034/1‑145 (MQ=18) gAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa > 8:215897/1‑118 (MQ=255) gAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaa < 7:215897/118‑1 (MQ=255) aaaaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGAcgcg < 1:175856/148‑1 (MQ=255) cgAGCGGAGTATAGTGCGCATCCACGGACGCGTGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGa < 1:27914/149‑1 (MQ=255) cGGAGTATCCTGCGCATCCACGGACTCGGTGAGGAGCATCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTAGGTTCTTATAATTACCGCGGAACCAATAAAATTTTGATGAATATACGCACTACCCTGACGCGGGGTGGagca < 2:168229/148‑1 (MQ=9) tataGTGCGCTTCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTtgat < 2:330856/108‑1 (MQ=11) tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt < 5:177523/105‑1 (MQ=255) tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt > 6:177523/1‑105 (MQ=255) tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt < 2:213352/105‑1 (MQ=255) tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt > 1:213352/1‑105 (MQ=255) tataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTAGGTTCAAATCCGGCCCCAGCAACCAATTAAAATTtgatgta > 2:264623/1‑110 (MQ=255) tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGTAACCAATAAAAATTTGATCAATTAAATCAGTACGGTGACGCGGGGTGGAGCAGCCt > 6:188728/1‑108 (MQ=14) aGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTAATAAATTAAAGCAGTACGGTGACGCGGGGTGGagcagc > 2:240544/1‑105 (MQ=14) tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGagc > 6:284859/1‑73 (MQ=255) aCCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACTCTGGGTGGAGCAGCCTGGTAGCTCGTCGGGATCATAACCCGAAGGTCGTCTGTTCAAATCCGGCCACCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACg < 7:64756/143‑1 (MQ=18) aCCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCAGGACAAAGCAACAAATCAAATTTGATGAAGTAAAAGCAGTACg > 8:64756/1‑143 (MQ=18) aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAttatt < 4:29397/117‑5 (MQ=25) aaTTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAttatt > 3:29397/1‑113 (MQ=25) | AAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGTAAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACG > NC_000913/2947319‑2947602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |