Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,153,420 G→A 7.3% intergenic (‑322/+276) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,153,4200GA 7.3% 248.3 / 7.1 82intergenic (‑322/+276)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base G (31/45);  new base A (3/3);  total (34/48)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

CATTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCC  >  NC_000913/4153272‑4153562
                                                                                                                                                    |                                                                                                                                              
cATTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATg                                                                                                                                                >  1:76957/1‑149 (MQ=255)
 aTTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc                                                                                                                                               <  2:39533/149‑1 (MQ=255)
  ttAAATTCACGACGCTTTGAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCGAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                                                                                                              <  6:176545/149‑1 (MQ=255)
  ttAAATTCACGAAGCTTTAAATAAGCCTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                                                                                                              >  7:44451/1‑149 (MQ=255)
      aTTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGGTGCGgcgc                                                                                                                                          >  8:14754/1‑149 (MQ=255)
      aTTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGgcgc                                                                                                                                          >  6:99886/1‑149 (MQ=255)
         cACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa                                                                                                                                        <  1:388181/148‑1 (MQ=255)
         cACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATAAGgcgctc                                                                                                                                        <  1:102650/148‑3 (MQ=255)
          acgacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCTCTGGAGTTTGGCAACAGTGCCTGATGAGGCGCGAgc                                                                                                                                      >  7:178963/1‑149 (MQ=255)
           cgacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcg                                                                                                                                     >  4:214478/1‑149 (MQ=255)
            gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    >  4:227690/1‑149 (MQ=255)
            gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    <  4:192730/149‑1 (MQ=255)
            gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    >  2:19029/1‑149 (MQ=255)
                cTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCCCATACGACAATATTGCGGCACACGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                >  1:179597/1‑149 (MQ=25)
                 tttAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                               >  1:59038/1‑149 (MQ=255)
                 tttAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                               >  2:175261/1‑149 (MQ=255)
                    aaaTAAGCGTAACTTATGGAAATTTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                             <  4:24251/148‑1 (MQ=255)
                      aTAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                          >  7:66584/1‑149 (MQ=255)
                         aGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                       <  3:126750/149‑1 (MQ=255)
                         aGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                       <  7:247875/149‑1 (MQ=255)
                            gTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAg                                                                                                                    <  2:76957/149‑1 (MQ=255)
                             tAACTTATGGAAATGTTAAAAAATCTCCCCAAGTAACACCACAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGt                                                                                                                   <  6:346694/149‑1 (MQ=255)
                             tAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGt                                                                                                                   <  3:104769/149‑1 (MQ=255)
                                cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  1:19029/149‑1 (MQ=255)
                                cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  3:403/149‑1 (MQ=255)
                                cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  4:332041/149‑1 (MQ=255)
                                   aTGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGc                                                                                                              <  1:175261/148‑1 (MQ=255)
                                    tGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCa                                                                                                             <  8:44451/148‑1 (MQ=255)
                                      gAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATc                                                                                                           <  3:344640/148‑1 (MQ=255)
                                         aTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                       <  4:256856/149‑1 (MQ=255)
                                         aTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                       <  5:99886/149‑1 (MQ=255)
                                            ttAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGGTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                     <  3:264107/148‑1 (MQ=255)
                                                aaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                <  1:279142/149‑1 (MQ=255)
                                                 aaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACt                                                                                               <  2:186483/149‑1 (MQ=255)
                                                   aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgt                                                                                             >  6:351674/1‑149 (MQ=255)
                                                    tCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtc                                                                                            >  1:270745/1‑149 (MQ=255)
                                                       ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtaggt                                                                                         >  1:273486/1‑149 (MQ=255)
                                                        cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                        <  7:28712/149‑1 (MQ=255)
                                                        cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTAGGCAACAGTGACGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                        >  1:275925/1‑149 (MQ=255)
                                                           aaGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGa                                                                                     <  5:351674/149‑1 (MQ=255)
                                                            aGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAt                                                                                    <  3:214478/149‑1 (MQ=255)
                                                             gTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATa                                                                                   <  8:119660/149‑1 (MQ=255)
                                                              tAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAt                                                                                    >  3:180795/1‑147 (MQ=255)
                                                                acacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAGCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 <  1:165506/148‑1 (MQ=255)
                                                                  acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAAAGTTGGGCATCCTTTGCGTCACTCTCCGTCGGATAAGaag                                                                              >  7:337150/1‑147 (MQ=14)
                                                                  acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTAGTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATg                                                                              >  7:350206/1‑149 (MQ=255)
                                                                    cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc                                                                                                                                   <  3:32320/94‑1 (MQ=40)
                                                                    cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc                                                                                                                                   >  4:32320/1‑94 (MQ=40)
                                                                    cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCGTGGTTTGAGTCACTGTCGGTCGGATAAGATgcg                                                                            >  7:177902/1‑149 (MQ=255)
                                                                       aGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCaa                                                                         <  3:312738/149‑1 (MQ=39)
                                                                          tgtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGt                                                                       >  3:134124/1‑148 (MQ=38)
                                                                            tAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                <  3:310353/121‑1 (MQ=34)
                                                                            tAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                >  4:310353/1‑121 (MQ=34)
                                                                             aGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc                                                                    <  7:321805/148‑1 (MQ=38)
                                                                                 cGGATAAGATGCTCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATc                                                               <  1:102771/149‑1 (MQ=37)
                                                                                     tAAGATGCGCAAGTATCGAATCCGACTTTATTGCGGCACTGGAGTTGTCCAACATTGCCGGATGCTTCGCGAGCGCCTTATCCGGACTACAGTTGGGCATCTTTTGAGTCTCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGAc                                                           <  2:275925/149‑1 (MQ=255)
                                                                                      aaGCTGCGCAAGTCTCGCATCCGACATTATTGCGGCACGGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGGCGGATAAGATGCGCAAGTATCGCAt                                                                <  7:366533/143‑1 (MQ=11)
                                                                                      aaGATGCGCAAGTATCGCATCCGACATTAATGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAGCCGGCATACAGTTGGGCATCGTGGGAGTCGCTGTCGGTCGGATAAGATGCGCAAGTATCGCag                                                                >  8:366533/1‑142 (MQ=11)
                                                                                        gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa                                                          <  8:33425/147‑1 (MQ=37)
                                                                                        gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa                                                          >  7:33425/1‑147 (MQ=37)
                                                                                            cgcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACattatt                                                     <  6:153593/148‑1 (MQ=37)
                                                                                               aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 >  8:323630/1‑117 (MQ=35)
                                                                                               aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 <  7:323630/117‑1 (MQ=35)
                                                                                                    tagaaTCCGACATTATTGCGGCACTGGAGTTTGTCAACAGTGCCGTATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg                                            <  1:235772/145‑1 (MQ=255)
                                                                                                    tCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgc                                                                             >  7:335274/1‑116 (MQ=35)
                                                                                                    ccgatctcGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTCGCCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg                                            <  5:288876/142‑1 (MQ=255)
                                                                                                      gCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGGTGCGGCGCGAGCGGCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                          <  7:14754/149‑1 (MQ=255)
                                                                                                            gACATTATTGCGGCACTGGAGATTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATAAGGCGTACAGTTGGGCATCGGATGAGAAAATGTCGTTAGGATAGGATGCGCGGGGGTCGCCTGCGACAGTGTTGGGGCGCTGGAgatcgg                                    >  6:288876/1‑144 (MQ=2)
                                                                                                                  attGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGCTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACa                               <  4:180795/148‑1 (MQ=37)
                                                                                                                      cGGCACTGGAGTTTGGCAACTGTGCCGGATGCGGCGCGATCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGGCGGATAAGATGCGCAAGTATCGCATCCGACTTTATTGCGGCACTGGAGTTTGGCAACAGTGcc                          <  2:385157/149‑1 (MQ=255)
                                                                                                                            tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCAt                                                                >  7:241528/1‑105 (MQ=17)
                                                                                                                              gAGTTTGGCAACAGTTCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCTCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                   <  8:157176/148‑1 (MQ=255)
                                                                                                                               aGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcggc                 <  4:134124/149‑1 (MQ=39)
                                                                                                                                    ggCTACAGTGCAGGATGCGGCTCGAGCTCCTTATCATTCATACATTTGGGAATCTTTTGATTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa             <  4:109672/148‑1 (MQ=14)
                                                                                                                                     gCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgc           <  8:110623/149‑1 (MQ=39)
                                                                                                                                       ctgtaggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc         <  4:327867/143‑1 (MQ=14)
                                                                                                                                       aaCAGTGGAGTATGCGGCTCGATCGCCTTATCAGGCCTACAGTTGGGCATAGTTTGAGTAACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc         <  8:238498/149‑1 (MQ=14)
                                                                                                                                       aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGt                                        >  1:351282/1‑118 (MQ=39)
                                                                                                                                        aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                          >  1:326107/1‑115 (MQ=39)
                                                                                                                                        aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc        >  7:301419/1‑149 (MQ=39)
                                                                                                                                        aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc        <  8:66584/149‑1 (MQ=39)
                                                                                                                                        aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGAACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGTGcc        >  8:83259/1‑149 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                         <  1:372727/127‑1 (MQ=14)
                                                                                                                                             gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                         >  2:372727/1‑127 (MQ=14)
                                                                                                                                             gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATc   >  3:262465/1‑149 (MQ=14)
                                                                                                                                             gCCGGATAAGGCGCTCGCGCCGCATCCGGCCGACAGTTGAGCATCGTGTGAGTCCCTGTCGGGCGGATAAGATGCGCAAGTATCGCATCCGACCTTATTGCGGCCCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATc   >  5:165547/1‑149 (MQ=14)
                                                                                                                                               atgaagcggccCGAGCTCCTTATCCGGCCTACAGTTGCGCATCCACTGATTCACTGTAGGTCGGATAAGATTCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATTCGGCGCGAGCGCCTTATcc  <  7:182885/143‑1 (MQ=11)
                                                                                                                                                    |                                                                                                                                              
CATTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCC  >  NC_000913/4153272‑4153562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: