Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,153,421 C→A 8.1% intergenic (‑323/+275) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,153,4210CA 8.1% 247.2 / 9.2 82intergenic (‑323/+275)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base C (30/45);  new base A (4/3);  total (34/48)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

ATTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCC  >  NC_000913/4153273‑4153562
                                                                                                                                                    |                                                                                                                                             
aTTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgc                                                                                                                                               <  2:39533/149‑1 (MQ=255)
 ttAAATTCACGACGCTTTGAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCGAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                                                                                                              <  6:176545/149‑1 (MQ=255)
 ttAAATTCACGAAGCTTTAAATAAGCCTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                                                                                                              >  7:44451/1‑149 (MQ=255)
     aTTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGGTGCGgcgc                                                                                                                                          >  8:14754/1‑149 (MQ=255)
     aTTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGgcgc                                                                                                                                          >  6:99886/1‑149 (MQ=255)
        cACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa                                                                                                                                        <  1:388181/148‑1 (MQ=255)
        cACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATAAGgcgctc                                                                                                                                        <  1:102650/148‑3 (MQ=255)
         acgacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCTCTGGAGTTTGGCAACAGTGCCTGATGAGGCGCGAgc                                                                                                                                      >  7:178963/1‑149 (MQ=255)
          cgacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcg                                                                                                                                     >  4:214478/1‑149 (MQ=255)
           gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    <  4:192730/149‑1 (MQ=255)
           gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    >  4:227690/1‑149 (MQ=255)
           gacgCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                                                                                                                    >  2:19029/1‑149 (MQ=255)
               cTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCCCATACGACAATATTGCGGCACACGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                >  1:179597/1‑149 (MQ=25)
                tttAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                               >  1:59038/1‑149 (MQ=255)
                tttAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                               >  2:175261/1‑149 (MQ=255)
                   aaaTAAGCGTAACTTATGGAAATTTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                             <  4:24251/148‑1 (MQ=255)
                     aTAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                          >  7:66584/1‑149 (MQ=255)
                        aGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                       <  3:126750/149‑1 (MQ=255)
                        aGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTa                                                                                                                       <  7:247875/149‑1 (MQ=255)
                           gTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAg                                                                                                                    <  2:76957/149‑1 (MQ=255)
                            tAACTTATGGAAATGTTAAAAAATCTCCCCAAGTAACACCACAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGt                                                                                                                   <  6:346694/149‑1 (MQ=255)
                            tAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGt                                                                                                                   <  3:104769/149‑1 (MQ=255)
                               cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  3:403/149‑1 (MQ=255)
                               cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  4:332041/149‑1 (MQ=255)
                               cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                <  1:19029/149‑1 (MQ=255)
                                  aTGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGc                                                                                                              <  1:175261/148‑1 (MQ=255)
                                   tGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCa                                                                                                             <  8:44451/148‑1 (MQ=255)
                                     gAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATc                                                                                                           <  3:344640/148‑1 (MQ=255)
                                        aTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                       <  4:256856/149‑1 (MQ=255)
                                        aTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                       <  5:99886/149‑1 (MQ=255)
                                           ttAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGGTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                     <  3:264107/148‑1 (MQ=255)
                                               aaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                <  1:279142/149‑1 (MQ=255)
                                                aaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACt                                                                                               <  2:186483/149‑1 (MQ=255)
                                                  aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgt                                                                                             >  6:351674/1‑149 (MQ=255)
                                                   tCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtc                                                                                            >  1:270745/1‑149 (MQ=255)
                                                      ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtaggt                                                                                         >  1:273486/1‑149 (MQ=255)
                                                       cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                        <  7:28712/149‑1 (MQ=255)
                                                       cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTAGGCAACAGTGACGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                        >  1:275925/1‑149 (MQ=255)
                                                          aaGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGa                                                                                     <  5:351674/149‑1 (MQ=255)
                                                           aGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAt                                                                                    <  3:214478/149‑1 (MQ=255)
                                                            gTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATa                                                                                   <  8:119660/149‑1 (MQ=255)
                                                             tAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAt                                                                                    >  3:180795/1‑147 (MQ=255)
                                                               acacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAGCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 <  1:165506/148‑1 (MQ=255)
                                                                 acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAAAGTTGGGCATCCTTTGCGTCACTCTCCGTCGGATAAGaag                                                                              >  7:337150/1‑147 (MQ=14)
                                                                 acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTAGTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATg                                                                              >  7:350206/1‑149 (MQ=255)
                                                                   cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc                                                                                                                                   <  3:32320/94‑1 (MQ=40)
                                                                   cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc                                                                                                                                   >  4:32320/1‑94 (MQ=40)
                                                                   cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCGTGGTTTGAGTCACTGTCGGTCGGATAAGATgcg                                                                            >  7:177902/1‑149 (MQ=255)
                                                                      aGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCaa                                                                         <  3:312738/149‑1 (MQ=39)
                                                                         tgtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGt                                                                       >  3:134124/1‑148 (MQ=38)
                                                                           tAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                >  4:310353/1‑121 (MQ=34)
                                                                           tAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                <  3:310353/121‑1 (MQ=34)
                                                                            aGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc                                                                    <  7:321805/148‑1 (MQ=38)
                                                                                cGGATAAGATGCTCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATc                                                               <  1:102771/149‑1 (MQ=37)
                                                                                    tAAGATGCGCAAGTATCGAATCCGACTTTATTGCGGCACTGGAGTTGTCCAACATTGCCGGATGCTTCGCGAGCGCCTTATCCGGACTACAGTTGGGCATCTTTTGAGTCTCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGAc                                                           <  2:275925/149‑1 (MQ=255)
                                                                                     aaGCTGCGCAAGTCTCGCATCCGACATTATTGCGGCACGGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGGCGGATAAGATGCGCAAGTATCGCAt                                                                <  7:366533/143‑1 (MQ=11)
                                                                                     aaGATGCGCAAGTATCGCATCCGACATTAATGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAGCCGGCATACAGTTGGGCATCGTGGGAGTCGCTGTCGGTCGGATAAGATGCGCAAGTATCGCag                                                                >  8:366533/1‑142 (MQ=11)
                                                                                       gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa                                                          <  8:33425/147‑1 (MQ=37)
                                                                                       gATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACa                                                          >  7:33425/1‑147 (MQ=37)
                                                                                           cgcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACattatt                                                     <  6:153593/148‑1 (MQ=37)
                                                                                              aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 >  8:323630/1‑117 (MQ=35)
                                                                                              aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                 <  7:323630/117‑1 (MQ=35)
                                                                                                   tagaaTCCGACATTATTGCGGCACTGGAGTTTGTCAACAGTGCCGTATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg                                            <  1:235772/145‑1 (MQ=255)
                                                                                                   tCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgc                                                                             >  7:335274/1‑116 (MQ=35)
                                                                                                   ccgatctcGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTCGCCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTg                                            <  5:288876/142‑1 (MQ=255)
                                                                                                     gCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGGTGCGGCGCGAGCGGCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                          <  7:14754/149‑1 (MQ=255)
                                                                                                           gACATTATTGCGGCACTGGAGATTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATAAGGCGTACAGTTGGGCATCGGATGAGAAAATGTCGTTAGGATAGGATGCGCGGGGGTCGCCTGCGACAGTGTTGGGGCGCTGGAgatcgg                                    >  6:288876/1‑144 (MQ=2)
                                                                                                                 attGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGCTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACa                               <  4:180795/148‑1 (MQ=37)
                                                                                                                     cGGCACTGGAGTTTGGCAACTGTGCCGGATGCGGCGCGATCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGGCGGATAAGATGCGCAAGTATCGCATCCGACTTTATTGCGGCACTGGAGTTTGGCAACAGTGcc                          <  2:385157/149‑1 (MQ=255)
                                                                                                                           tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCAt                                                                >  7:241528/1‑105 (MQ=17)
                                                                                                                             gAGTTTGGCAACAGTTCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCTCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                   <  8:157176/148‑1 (MQ=255)
                                                                                                                              aGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcggc                 <  4:134124/149‑1 (MQ=39)
                                                                                                                                   ggCTACAGTGCAGGATGCGGCTCGAGCTCCTTATCATTCATACATTTGGGAATCTTTTGATTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa             <  4:109672/148‑1 (MQ=14)
                                                                                                                                    gCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgc           <  8:110623/149‑1 (MQ=39)
                                                                                                                                      ctgtaggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc         <  4:327867/143‑1 (MQ=14)
                                                                                                                                      aaCAGTGGAGTATGCGGCTCGATCGCCTTATCAGGCCTACAGTTGGGCATAGTTTGAGTAACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc         <  8:238498/149‑1 (MQ=14)
                                                                                                                                      aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGt                                        >  1:351282/1‑118 (MQ=39)
                                                                                                                                       aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                          >  1:326107/1‑115 (MQ=39)
                                                                                                                                       aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc        >  7:301419/1‑149 (MQ=39)
                                                                                                                                       aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc        <  8:66584/149‑1 (MQ=39)
                                                                                                                                       aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGAACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGTGcc        >  8:83259/1‑149 (MQ=255)
                                                                                                                                            gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                         <  1:372727/127‑1 (MQ=14)
                                                                                                                                            gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                         >  2:372727/1‑127 (MQ=14)
                                                                                                                                            gCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATc   >  3:262465/1‑149 (MQ=14)
                                                                                                                                            gCCGGATAAGGCGCTCGCGCCGCATCCGGCCGACAGTTGAGCATCGTGTGAGTCCCTGTCGGGCGGATAAGATGCGCAAGTATCGCATCCGACCTTATTGCGGCCCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATc   >  5:165547/1‑149 (MQ=14)
                                                                                                                                              atgaagcggccCGAGCTCCTTATCCGGCCTACAGTTGCGCATCCACTGATTCACTGTAGGTCGGATAAGATTCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATTCGGCGCGAGCGCCTTATcc  <  7:182885/143‑1 (MQ=11)
                                                                                                                                                    |                                                                                                                                             
ATTAAATTCACGACGCTTTAAATAAGCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCC  >  NC_000913/4153273‑4153562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: