mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L001_R1_001.good.fq | 552,524 | 78,081,228 | 100.0% | 141.3 bases | 149 bases | 97.2% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L001_R2_001.good.fq | 552,524 | 77,858,101 | 100.0% | 140.9 bases | 149 bases | 97.4% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L002_R1_001.good.fq | 510,419 | 72,177,728 | 100.0% | 141.4 bases | 149 bases | 97.3% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L002_R2_001.good.fq | 510,419 | 71,995,181 | 100.0% | 141.1 bases | 149 bases | 97.0% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L003_R1_001.good.fq | 538,245 | 76,050,901 | 100.0% | 141.3 bases | 149 bases | 97.1% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L003_R2_001.good.fq | 538,245 | 75,837,141 | 100.0% | 140.9 bases | 149 bases | 97.1% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L004_R1_001.good.fq | 553,638 | 78,228,218 | 100.0% | 141.3 bases | 149 bases | 97.3% |
| errors | Plate-1-BOP-1000-RPE-END-3_S31_L004_R2_001.good.fq | 553,638 | 78,034,350 | 100.0% | 140.9 bases | 149 bases | 97.1% |
| total | 4,309,652 | 608,262,848 | 100.0% | 141.1 bases | 149 bases | 97.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 118.8 | 4.0 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 59848 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 135 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.009 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.69071 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:35:25 10 Sep 2020 | 18:36:30 10 Sep 2020 | 1 minute 5 seconds |
| Read alignment to reference genome | 18:36:30 10 Sep 2020 | 18:44:39 10 Sep 2020 | 8 minutes 9 seconds |
| Preprocessing alignments for candidate junction identification | 18:44:39 10 Sep 2020 | 18:45:44 10 Sep 2020 | 1 minute 5 seconds |
| Preliminary analysis of coverage distribution | 18:45:44 10 Sep 2020 | 18:48:10 10 Sep 2020 | 2 minutes 26 seconds |
| Identifying junction candidates | 18:48:10 10 Sep 2020 | 18:49:55 10 Sep 2020 | 1 minute 45 seconds |
| Re-alignment to junction candidates | 18:49:55 10 Sep 2020 | 18:52:26 10 Sep 2020 | 2 minutes 31 seconds |
| Resolving best read alignments | 18:52:26 10 Sep 2020 | 18:54:09 10 Sep 2020 | 1 minute 43 seconds |
| Creating BAM files | 18:54:09 10 Sep 2020 | 18:56:22 10 Sep 2020 | 2 minutes 13 seconds |
| Tabulating error counts | 18:56:22 10 Sep 2020 | 18:57:09 10 Sep 2020 | 47 seconds |
| Re-calibrating base error rates | 18:57:09 10 Sep 2020 | 18:57:11 10 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 18:57:11 10 Sep 2020 | 19:06:36 10 Sep 2020 | 9 minutes 25 seconds |
| Polymorphism statistics | 19:06:36 10 Sep 2020 | 19:06:36 10 Sep 2020 | 0 seconds |
| Output | 19:06:36 10 Sep 2020 | 19:06:58 10 Sep 2020 | 22 seconds |
| Total | 31 minutes 33 seconds | ||
