Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,734,215 A→G 6.8% S166G (AGC→GGC)  xylH → D‑xylose ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,734,2150AG 6.8% 172.5 / 3.5 59S166G (AGC→GGC) xylHD‑xylose ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base G (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCT  >  NC_000913/3734070‑3734359
                                                                                                                                                 |                                                                                                                                                
ggTTCTGGGACTGCTTCTCGGTGCCTGGTAAGGATGCTTTGTCCCGAACCGTAAAGACCCTTCATTTATAGTCACCCTCGCCTGCATTTTGTCATTTCGCGCCATCCTCATTTGCATCCACAACGGCACTCATGTATCCCCCACCAgc                                                                                                                                                >  5:29370/1‑148 (MQ=255)
      gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGCCTGTATCCCCCACCAGcgccgc                                                                                                                                           >  7:22502/1‑147 (MQ=255)
         aCTGCTTCTCGTTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGGATTTCGCGTCTTACTCTTTGGCATCACCAACGGCACGACTGTATGCCCCACCAGCGCCGCGATg                                                                                                                                       <  6:359526/148‑1 (MQ=255)
         aCTGCGTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATg                                                                                                                                       <  8:287773/148‑1 (MQ=255)
          cTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGt                                                                                                                                      <  6:238312/148‑1 (MQ=255)
             cTTCTCGGTGCTTGGAACGGATGGTGGGTCGCGTTCCGTATAGTCCCTTCATTTATTGTCACCGTCGCGTGATTGTTGGCATTTCGCGGCATACTGATTGGCATAACCAACGGCACGAGTGTATGCCCCACCAGCGCCGCTATGTc                                                                                                                                     <  2:1614/146‑1 (MQ=255)
                 tcGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAAt                                                                                                                               >  7:209349/1‑148 (MQ=255)
                 tcGGTGCATGGAACGGATGGTGGGTCGCGTACAGTAAATTCCCTTCATTTATTGTCAACCTCGCGGGCATGTTGCCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCGCCCACCAGCGCCGCGATGTCACAAAt                                                                                                                               >  4:75524/1‑148 (MQ=255)
                    gTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTgg                                                                                                                            <  3:134495/148‑1 (MQ=255)
                             aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTa                                                                                                                   <  7:156121/148‑1 (MQ=255)
                                gATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGCCTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAtct                                                                                                                >  1:263370/1‑148 (MQ=255)
                                 aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTAtctc                                                                                                               <  2:337321/148‑1 (MQ=255)
                                    gtggGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTccc                                                                                                             <  4:247528/147‑1 (MQ=255)
                                       ggTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTTGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGcc                                                                                                         <  7:406553/148‑1 (MQ=255)
                                                    aaaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGTGCAAAGCTATCTCCCCGCCAGTACCGGCtgc                                                                                             >  2:258032/1‑145 (MQ=255)
                                                      aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTa                                                                                                                   >  7:234907/1‑123 (MQ=255)
                                                      aGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTa                                                                                                                   <  8:234907/123‑1 (MQ=255)
                                                          ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGTGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCAtt                                                                                       <  1:258032/147‑1 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCGGCGCCGCGATGTCACAAATTgg                                                                                                                            >  4:89370/1‑107 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCGGCGCCGCGATGTCACAAATTgg                                                                                                                            >  4:84377/1‑107 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCGGCGCCGCGATGTCACAAATTgg                                                                                                                            <  3:89370/107‑1 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCGGCGCCGCGATGTCACAAATTgg                                                                                                                            <  3:84377/107‑1 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCcgcg                                                                                                                                          >  6:274283/1‑93 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCcgcg                                                                                                                                          <  5:274283/93‑1 (MQ=255)
                                                             tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcg                                                                                   >  2:299205/1‑148 (MQ=255)
                                                                 ttATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTg                                                                                                                             <  5:114306/102‑1 (MQ=255)
                                                                 ttATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTg                                                                                                                             >  6:114306/1‑102 (MQ=255)
                                                                  tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtca                                                                                            >  3:348026/1‑134 (MQ=255)
                                                                  tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtca                                                                                            <  4:348026/134‑1 (MQ=255)
                                                                           ccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCcgcg                                                                                                                                          >  8:285278/1‑79 (MQ=255)
                                                                           ccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCcgcg                                                                                                                                          <  7:285278/79‑1 (MQ=255)
                                                                                cgGGCATGTTGGCATTTCGCGGCTTACTCATTGGCAACACCAACGGCACCACTGTATCCCCCACCAGCGCCGCGATGTCAAAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTGCATCATTGGCGCGCTTGGATTAATGGCttt                                                                >  3:301986/1‑148 (MQ=255)
                                                                                      tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                          <  1:299205/148‑1 (MQ=255)
                                                                                       gTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggt                                                         >  3:108955/1‑148 (MQ=255)
                                                                                        ttGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggtt                                                        <  2:263370/148‑1 (MQ=255)
                                                                                          ggCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggttg                                                       <  8:328744/147‑1 (MQ=255)
                                                                                            cATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttg                                                              >  1:362934/1‑138 (MQ=255)
                                                                                            cATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttg                                                              <  2:362934/138‑1 (MQ=255)
                                                                                               ttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcgcgc                                                                                >  3:70410/1‑117 (MQ=255)
                                                                                               ttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcgcgc                                                                                <  4:70410/117‑1 (MQ=255)
                                                                                                      cATACTCATTGGGATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCACAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGga                                          <  7:324511/148‑1 (MQ=255)
                                                                                                        tACTCATTGGCCTCACCAACGGCACGACTGTATCACCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCgg                                           >  5:135546/1‑145 (MQ=255)
                                                                                                        tACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCa                                                    >  5:381706/1‑136 (MQ=255)
                                                                                                        tACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCa                                                    <  6:381706/136‑1 (MQ=255)
                                                                                                         aCTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTgg                                                                                                                            >  4:223677/1‑63 (MQ=255)
                                                                                                         aCTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTgg                                                                                                                            <  3:223677/63‑1 (MQ=255)
                                                                                                         aCTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaag                                        >  4:55289/1‑147 (MQ=255)
                                                                                                          cTCATTGGCATCACCAACGGCAAGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaaga                                       <  6:135546/147‑1 (MQ=255)
                                                                                                            cATTGGCATCACCAACGGCACGCCTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATg                                    >  8:390175/1‑148 (MQ=255)
                                                                                                               tGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATTGCTTTTGTTGGATGGCAAAGGCGCGGAAGAATgcgc                                 >  2:275891/1‑148 (MQ=255)
                                                                                                                 gCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCg                               >  7:222364/1‑148 (MQ=255)
                                                                                                                     cACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAg                           >  4:366848/1‑148 (MQ=255)
                                                                                                                       ccAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCCCAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCGTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGc                         >  7:327205/1‑148 (MQ=255)
                                                                                                                        cAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCt                        >  2:2345/1‑148 (MQ=255)
                                                                                                                             gCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAt                                                  >  1:334963/1‑117 (MQ=255)
                                                                                                                             gCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAt                                                  <  2:334963/117‑1 (MQ=255)
                                                                                                                                cGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGtt                 >  8:299980/1‑147 (MQ=255)
                                                                                                                                     gTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGACGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt           >  1:41027/1‑148 (MQ=255)
                                                                                                                                          ccccACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTcc       <  8:22502/147‑1 (MQ=255)
                                                                                                                                            ccaccaGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGc    >  6:76548/1‑148 (MQ=255)
                                                                                                                                              accaGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTGACAGTGTCCGGccg  >  8:175096/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTTCTGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCT  >  NC_000913/3734070‑3734359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑