Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,192,500	A→G	6.5%	I44I (ATT→ATC)	hflD ←	putative lysogenization regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,192,500	0	A	G	6.5%	184.7 / 3.6	61	I44I (ATT→ATC)	hflD	putative lysogenization regulator
Reads supporting (aligned to +/- strand):  ref base A (26/31);  new base G (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

ATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCC  >  NC_000913/1192354‑1192644                                                                                                                                                   |                                                                                                                                                 aTCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaa                                                                                                                                                 >  4:351515/1‑148 (MQ=255)     aGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaataat                                                                                                                                             <  7:273442/148‑1 (MQ=255)     aGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGAATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaataat                                                                                                                                             <  3:10100/148‑1 (MQ=255)               gtAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTtgagt                                                                                                                                   >  5:336752/1‑148 (MQ=255)                  gCGGGTTAATTCGGCGTTTAAGCCCTGGCGAGTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTtgagtgag                                                                                                                                <  8:285454/148‑1 (MQ=255)                     ggTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACg                                                                                                                             <  5:99453/148‑1 (MQ=255)                     ggTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACg                                                                                                                             <  3:316461/148‑1 (MQ=255)                      gTTAATTCGGCGTTTAAGCCCTGGCCACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACgt                                                                                                                            >  3:196703/1‑148 (MQ=255)                       ttAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACgtg                                                                                                                           >  5:195446/1‑148 (MQ=255)                           ttCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAgc                                                                                                                       >  8:34030/1‑148 (MQ=255)                              ggCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGGCGAGCTGGGGTTCATCTCAATAATACTGTTGAGTGAGACGTGTAgcgc                                                                                                                     <  3:222283/147‑1 (MQ=255)                               gCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCAt                                                                                                                   <  7:34030/148‑1 (MQ=255)                                cGTTTAAGCCCTGGCGACTGGTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATc                                                                                                                  <  5:52040/148‑1 (MQ=255)                                   ttAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCgg                                                                                                                >  3:103920/1‑147 (MQ=255)                                   ttAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCAAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCgg                                                                                                                >  2:190721/1‑147 (MQ=255)                                       gCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCC‑AAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATca                                                                                                           >  5:109313/1‑147 (MQ=255)                                         ccTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  7:348850/148‑1 (MQ=255)                                         ccTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  4:38036/148‑1 (MQ=255)                                         ccTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTGTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  3:59091/148‑1 (MQ=255)                                           tGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         >  1:26301/1‑146 (MQ=255)                                           tGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  2:26301/146‑1 (MQ=255)                                                    ctgGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTgg                                                                                               <  3:351515/147‑1 (MQ=255)                                                     tgGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGAAGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGGTGAGTGAGACGTGTAGCGCATCGGACTCACAATGCCCCTGGt                                                                                              >  5:263902/1‑147 (MQ=255)                                                       gCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAg                                                                                           <  4:161897/148‑1 (MQ=255)                                                        cATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCGATAATACTGTtg                                                                                                                                      <  6:12340/104‑1 (MQ=255)                                                        cATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCGATAATACTGTtg                                                                                                                                      <  6:17542/104‑1 (MQ=255)                                                        cATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCGATAATACTGTtg                                                                                                                                      >  5:17542/1‑104 (MQ=255)                                                        cATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCGATAATACTGTtg                                                                                                                                      >  5:12340/1‑104 (MQ=255)                                                            gAGCACGCCGAGCAGGGTTGCCAGACCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGGCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAg                                                                                       <  1:73170/147‑1 (MQ=255)                                                                aCGCCGAGCAGGGTTTCCAGCCCGCCGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCCTCACAATGCCCCTGGTGAGCGAgttgtt                                                                                  >  2:177545/1‑148 (MQ=255)                                                                aCGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaat                                                                                                                                                <  2:53070/86‑1 (MQ=255)                                                                aCGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaat                                                                                                                                                >  1:53070/1‑86 (MQ=255)                                                                aCGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAgttgtt                                                                                  <  2:298127/148‑1 (MQ=255)                                                                   ccGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCa                                                                               <  1:325889/148‑1 (MQ=255)                                                                    cGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         >  7:83235/1‑121 (MQ=255)                                                                    cGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  8:83235/121‑1 (MQ=255)                                                                     gAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCAcc                                                                             <  6:336752/148‑1 (MQ=255)                                                                         aGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGc                                                                         <  7:156146/148‑1 (MQ=255)                                                                           ggTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaa                                                                                                                                                 <  4:115553/74‑1 (MQ=255)                                                                           ggTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaa                                                                                                                                                 >  3:115553/1‑74 (MQ=255)                                                                            gTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAgttgt                                                                                   >  1:273119/1‑135 (MQ=255)                                                                            gTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAgttgt                                                                                   <  2:273119/135‑1 (MQ=255)                                                                            gTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTg                                                                      >  7:121599/1‑148 (MQ=255)                                                                               tCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCC‑AAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCg                                                                   <  6:109313/147‑1 (MQ=255)                                                                                ccAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAACCCGCCAGCGTCGAGCTGGGGTTCATATCAAAAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAAAGCCCCTGGTGAGCGAGTTGTTTCACCAGGCGTGCCg                                                                   <  6:181910/147‑1 (MQ=255)                                                                                  aGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGCCt                                                                >  5:11140/1‑148 (MQ=255)                                                                                   gCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaata                                                                                                                                               >  8:158120/1‑68 (MQ=255)                                                                                   gCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCaata                                                                                                                                               <  7:158120/68‑1 (MQ=255)                                                                                       gACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACa                                                            >  5:195191/1‑147 (MQ=255)                                                                                       gACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACa                                                            >  2:311131/1‑147 (MQ=255)                                                                                                        cGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAg                                          >  3:42916/1‑148 (MQ=255)                                                                                                         gCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAg                                          <  6:195446/147‑1 (MQ=255)                                                                                                          cTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAggg                                        <  6:11140/148‑1 (MQ=255)                                                                                                           tACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAGTACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         >  2:377857/1‑82 (MQ=255)                                                                                                           tACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATACTACTGTTGAGTGAGACGTGTAGCGCATCGGCATcaca                                                                                                         <  1:377857/82‑1 (MQ=255)                                                                                                              cGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAg                                          <  6:80092/142‑1 (MQ=255)                                                                                                              cGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAg                                          >  5:80092/1‑142 (MQ=255)                                                                                                                 cAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTc                                 <  1:311131/148‑1 (MQ=255)                                                                                                                    aaaCCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCa                                >  8:266617/1‑146 (MQ=255)                                                                                                                     aaCCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAg                             >  8:244461/1‑148 (MQ=255)                                                                                                                              cGTCGAGCTGTGGTTCATATCAATAATACTGTTGAGGGAGACGTGTAGCGGATCGGCATCACTATGCACCTGGGGAGGGAGTTGTTGCACCAGGCGTGCCGACTGACAAATAGCGGCCAGGGCGAGGGTGATGTCATAGTAATGCtt                     <  8:191734/147‑1 (MQ=255)                                                                                                                                     cTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGt             <  2:376777/148‑1 (MQ=255)                                                                                                                                     cTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGAACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGt             >  7:304691/1‑148 (MQ=255)                                                                                                                                        gggTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTc           <  6:195191/147‑1 (MQ=255)                                                                                                                                         ggTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCAc         >  6:321718/1‑148 (MQ=255)                                                                                                                                                atCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTAGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTcc  >  4:34750/1‑148 (MQ=255)                                                                                                                                                 tCAATAATACTGTTTATTGAGACGTGTAGCTCATCGGCATCACAATTCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCa                                                 <  2:186645/100‑1 (MQ=255)                                                                                                                                                  cAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCa                                                 >  3:128428/1‑99 (MQ=255)                                                                                                                                                  cAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCa                                                 <  4:128428/99‑1 (MQ=255)                                                                                                                                                  cAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGAGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAg                                                >  1:186645/1‑100 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ATCAAGCTGAGTGTGTAGCGGGTTAATTCGGCGTTTAAGCCCTGGCGACTGCTGGCATTGAGCACGCCGAGCAGGGTTTCCAGCCCGACGCGCAGGTTGGCTTCGCTACCGCCAAAAACCGCCAGCGTCGAGCTGGGGTTCATATCAATAATACTGTTGAGTGAGACGTGTAGCGCATCGGCATCACAATGCCCCTGGTGAGCGAGTTGTTGCACCAGGCGTGCCGACTGACAAATACCGGCCAGGGCGAGGGTGATGTCATAGTAATTCTTTGCCACGTTCACTGCTTCC  >  NC_000913/1192354‑1192644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑