Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,700,606	G→A	6.0%	A418T (GCT→ACT)	malX →	maltose and glucose‑specific PTS enzyme IIB component and IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,606	0	G	A	6.0%	214.5 / 2.6	66	A418T (GCT→ACT)	malX	maltose and glucose‑specific PTS enzyme IIB component and IIC component
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base A (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

CTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGT  >  NC_000913/1700462‑1700751                                                                                                                                                 |                                                                                                                                                  cTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTa                                                                                                                                                <  7:363265/148‑1 (MQ=255)    ggagTCACCATCGGAAATACCGACGGCAATATCATCGACTTCGTGGTGTTAGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTAt                                                                                                                                               <  3:199864/143‑1 (MQ=255)    ggCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTAt                                                                                                                                               <  5:314795/146‑1 (MQ=255)      cGTCACCATCGGTAATACGCACGGCAATATCATCTCCTGAGTGGTGTTCGGTTTTTAGCATGGTCTGTCAACCATGTGGTACTTGGTGCCAGTGGTGGCGTCAATCTGGTTTGTCGCTTACTACGTCATCTTCCGTTTCGCTATCAcc                                                                                                                                           <  7:293066/148‑1 (MQ=255)            cATCGGTAATACCGACGACAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCtt                                                                                                                                      >  3:152252/1‑147 (MQ=255)                      aCCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGaaaa                                                                                                                           >  3:176148/1‑148 (MQ=255)                           cGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCg                                                                                                                      <  4:341153/148‑1 (MQ=255)                           cGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCg                                                                                                                      <  1:56226/148‑1 (MQ=255)                            ggCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCgg                                                                                                                     <  8:288533/148‑1 (MQ=255)                                    catcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGgc                                                                                                                  <  4:173470/143‑1 (MQ=255)                                    catcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGgc                                                                                                                  >  3:173470/1‑143 (MQ=255)                                     atcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGa                                                                                                              >  2:341589/1‑146 (MQ=255)                                      tcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATc                                                                                                                                              <  5:235529/113‑1 (MQ=255)                                      tcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATc                                                                                                                                              >  6:235529/1‑113 (MQ=255)                                      tcGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAg                                                                                                           >  4:336030/1‑148 (MQ=255)                                          cTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGaa                                                                                                       <  4:176148/148‑1 (MQ=255)                                            tCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGt                                                                                                     >  7:210158/1‑148 (MQ=255)                                             cGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAg                                                                                                      <  7:365429/146‑1 (MQ=255)                                              gtggtgTTCGGTATTTTGCATGGGCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTg                                                                                                   <  4:120744/148‑1 (MQ=255)                                                        gTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAAt                                                                                         <  2:140577/148‑1 (MQ=255)                                                            tttGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCt                                                                                                                                >  1:174842/1‑105 (MQ=255)                                                            tttGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCt                                                                                                                                <  2:174842/105‑1 (MQ=255)                                                            tttGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGa                                                                                      >  7:118716/1‑147 (MQ=255)                                                                    gTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGt                                                                             <  4:152252/148‑1 (MQ=255)                                                                      cTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCAccc                                                                                                                                          >  2:318584/1‑85 (MQ=255)                                                                      cTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCAccc                                                                                                                                          <  1:318584/85‑1 (MQ=255)                                                                        gTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCaa                                                                                          <  7:106709/131‑1 (MQ=255)                                                                        gTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCaa                                                                                          >  8:106709/1‑131 (MQ=255)                                                                         tCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTg                                                                           <  8:118716/145‑1 (MQ=255)                                                                         tCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCg                                                                        >  8:404854/1‑148 (MQ=255)                                                                         tCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCg                                                                        >  1:144353/1‑148 (MQ=255)                                                                               aaGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTgcgc                                                                  >  3:173910/1‑148 (MQ=255)                                                                                aGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCACTCGACACAGCCGTTGCCGGTgcgac                                                                 >  4:211043/1‑146 (MQ=255)                                                                                     tACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGt                                                             >  5:260458/1‑147 (MQ=255)                                                                                     tACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGt                                                             >  4:193255/1‑147 (MQ=255)                                                                                           gTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAg                                                      <  4:173910/148‑1 (MQ=255)                                                                                           gTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAg                                                      <  7:151203/148‑1 (MQ=255)                                                                                               cAGTGGTGGCGGCAATCTGGGTTGGCGTTTACTACGGCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTa                                                  <  3:328441/148‑1 (MQ=255)                                                                                                   ggtggCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAAc                                              >  5:144735/1‑148 (MQ=255)                                                                                                              tCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAAt                                   >  8:311105/1‑148 (MQ=255)                                                                                                               cTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGt                                                             <  5:140548/121‑1 (MQ=255)                                                                                                               cTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGt                                                             >  6:140548/1‑121 (MQ=255)                                                                                                                  gTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGcgcg                                                                                                               >  7:37835/1‑68 (MQ=255)                                                                                                                  gTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGcgcg                                                                                                               <  8:37835/68‑1 (MQ=255)                                                                                                                     tGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGa                             <  8:210158/147‑1 (MQ=255)                                                                                                                      gTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCg                                                                              <  5:322608/97‑1 (MQ=255)                                                                                                                      gTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCg                                                                              >  6:322608/1‑97 (MQ=255)                                                                                                                        cGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGCGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCa                         >  1:161398/1‑148 (MQ=255)                                                                                                                        cGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGAGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCa                         >  6:194732/1‑148 (MQ=255)                                                                                                                           ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGAt                                                                                                             <  7:255014/61‑1 (MQ=255)                                                                                                                           ttACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGAt                                                                                                             >  8:255014/1‑61 (MQ=255)                                                                                                                              ctacGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGa                                                                                      <  1:401120/81‑1 (MQ=255)                                                                                                                              ctacGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGa                                                                                      >  2:401120/1‑81 (MQ=255)                                                                                                                                 cGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCg                  <  2:144353/146‑1 (MQ=255)                                                                                                                                       cTTCCGTTTCACTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGa           >  5:224785/1‑147 (MQ=255)                                                                                                                                       cTTCCGTTTCACTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGa           >  5:219152/1‑147 (MQ=255)                                                                                                                                         tCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCg                                                                <  6:232670/92‑1 (MQ=255)                                                                                                                                         tCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCg                                                                >  5:232670/1‑92 (MQ=255)                                                                                                                                         tCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa        >  2:155354/1‑148 (MQ=255)                                                                                                                                          ccGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa        >  5:379092/1‑147 (MQ=255)                                                                                                                                           cGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAata      >  5:10711/1‑148 (MQ=255)                                                                                                                                           cGTTTCACTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAata      <  6:224785/148‑1 (MQ=255)                                                                                                                                           cGTTTCACTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAata      <  6:219152/148‑1 (MQ=255)                                                                                                                                             tttCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATAtt    >  5:300991/1‑148 (MQ=255)                                                                                                                                             tttCGCTATCACCCGCTTCAATCTGAAAAACCCGGGGCGCGATAGCGAAGTTCCCAGATCAATCGATAAACACGTTGCCGGTGCGCCGGTTAAATCAGGTTAAAACTTACCTGCAAACCTCGAAGCATAAGGAGTTACCTAAAATAtt    >  6:105833/1‑148 (MQ=255)                                                                                                                                                cGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGt  <  7:404854/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CTCGGCGTCACCATCGGTAATACCGACGGCAATATCATCGACTTCGTGGTGTTCGGTATTTTGCATGGTCTGTCAACCAAGTGGTACATGGTGCCAGTGGTGGCGGCAATCTGGTTTGTCGTTTACTACGTCATCTTCCGTTTCGCTATCACCCGCTTCAATCTGAAAACCCCGGGGCGCGATAGCGAAGTTGCCAGCTCAATCGAAAAAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGT  >  NC_000913/1700462‑1700751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑