Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,724,105	C→T	6.6%	A662V (GCA→GTA)	ydhK →	putative efflux protein (PET) component of YdhJK efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,724,105	0	C	T	6.6%	193.3 / 2.7	60	A662V (GCA→GTA)	ydhK	putative efflux protein (PET) component of YdhJK efflux pump
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

GCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCC  >  NC_000913/1723961‑1724248                                                                                                                                                 |                                                                                                                                                gCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGcacc                                                                                                                                              <  3:145704/148‑1 (MQ=255)        gCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGc                                                                                                                                         >  3:136900/1‑146 (MQ=255)          aaaaaTCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAgg                                                                                                                                     <  8:135825/148‑1 (MQ=255)            aaaTCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTa                                                                                                                                   >  4:99942/1‑148 (MQ=255)            aaaTCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTa                                                                                                                                   >  3:234983/1‑148 (MQ=255)            aaaTCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTa                                                                                                                                   >  1:232474/1‑148 (MQ=255)                gacttggggcCAGACTTGAAGATTTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCTCGCTGCGGGCTGTGTGCTGGCGGGTATTGTCTGGCGGCTGTAGTGCTCGCTTTGGCTACTTGAGCAAGCTCGACCGCAAGGTACGc                                                                                                                                <  2:49112/140‑1 (MQ=255)                    ggcggcCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGcc                                                                                                                           >  5:343475/1‑148 (MQ=255)                         ccACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTctta                                                                                                                      <  1:158998/148‑1 (MQ=255)                         ccACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTctta                                                                                                                      <  2:232474/148‑1 (MQ=255)                         ccACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTctta                                                                                                                      <  4:136900/148‑1 (MQ=255)                           acacTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaat                                                                                                                    <  7:19719/148‑1 (MQ=255)                            cacTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCaa                                                                                                                                       >  2:24470/1‑128 (MQ=255)                            cacTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCaa                                                                                                                                       <  1:24470/128‑1 (MQ=255)                             acTAGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCCACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaatta                                                                                                                  <  6:356205/148‑1 (MQ=255)                              cTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaattac                                                                                                                 >  8:233048/1‑148 (MQ=255)                               ttGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGagcaagca                                                                                                                                                >  2:181259/1‑116 (MQ=255)                               ttGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGagcaagca                                                                                                                                                <  1:181259/116‑1 (MQ=255)                                     aaTTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaattacttaatt                                                                                                           <  4:234983/147‑1 (MQ=255)                                      aTTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCttaattacttaattac                                                                                                         <  2:156868/148‑1 (MQ=255)                                       ttACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTaca                                                                                                        <  6:343475/148‑1 (MQ=255)                                         aCAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGTACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTacac                                                                                                       >  6:379243/1‑147 (MQ=255)                                            gCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTAcacca                                                                                                     >  4:184447/1‑146 (MQ=255)                                            gCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTAcacca                                                                                                     >  7:318197/1‑146 (MQ=255)                                                 tttGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCAt                                                                                              <  2:178432/148‑1 (MQ=255)                                                  ttGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCa                                                                                               >  6:197945/1‑146 (MQ=255)                                                       aCAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGTAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaatt                                                                                                                   >  6:351071/1‑121 (MQ=255)                                                       aCAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGTAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTcttaatt                                                                                                                   <  5:351071/121‑1 (MQ=255)                                                        cAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTc                                                                                       <  8:318197/148‑1 (MQ=255)                                                          gCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTAAATTACACCACAGGCATAGCGTTcac                                                                                     <  8:192194/148‑1 (MQ=255)                                                                     catcatcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACATGCATAGCGTTCaccgccaccgcc                                                                           >  2:103898/1‑147 (MQ=255)                                                                     catcatcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCaccgccaccgcc                                                                           >  7:296249/1‑147 (MQ=255)                                                                     catcatcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCaccgccaccgcc                                                                           >  2:47119/1‑147 (MQ=255)                                                                        catcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTCCGCTGGCCTCTTAATTACTTAATTACACCACAGGCCTAGCGTTCACCGCCACCGCCCAGc                                                                       >  3:282910/1‑148 (MQ=255)                                                                        catcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGc                                                                       >  4:174566/1‑148 (MQ=255)                                                                        catcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGc                                                                       >  3:74174/1‑148 (MQ=255)                                                                         atcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCCCCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACAACAGGCATAGCGTTCACCGCCACCGCCCAGCg                                                                      >  5:199045/1‑148 (MQ=255)                                                                         atcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGc                                                                       <  8:325712/147‑1 (MQ=255)                                                                          tcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGc                                                                                                                                <  8:328313/89‑1 (MQ=255)                                                                          tcaACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGc                                                                                                                                >  7:328313/1‑89 (MQ=255)                                                                            aaCCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGtt                                                                   >  7:382426/1‑148 (MQ=255)                                                                             aCCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGttt                                                                  <  7:76128/148‑1 (MQ=255)                                                                             aCCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATCACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGttt                                                                  <  6:66648/148‑1 (MQ=255)                                                                               cTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAg                                                                >  6:103387/1‑148 (MQ=255)                                                                                tGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAgg                                                               >  8:303725/1‑148 (MQ=255)                                                                                        gTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCgg                                                       <  7:303725/148‑1 (MQ=255)                                                                                         tGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGa                                                      <  1:317074/148‑1 (MQ=255)                                                                                            gCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCATCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCCGCGGTTTAGGTTGATCGGACa                                                    >  8:198092/1‑147 (MQ=255)                                                                                                gcggcAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACAtatta                                               <  7:233048/148‑1 (MQ=255)                                                                                                gcggcAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACAtatta                                               <  1:47119/148‑1 (MQ=255)                                                                                                gcggcAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACAtatt                                                <  5:103387/147‑1 (MQ=255)                                                                                                gcggcAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACAtatt                                                <  1:103898/147‑1 (MQ=255)                                                                                                      aTTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATcgccg                                         >  5:305626/1‑148 (MQ=255)                                                                                                           cTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAAc                                    >  6:124103/1‑148 (MQ=255)                                                                                                             ggcggcTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGt                                  >  8:279970/1‑148 (MQ=255)                                                                                                              gcggcTGTACTGCTCGCTTTCGCAACTTGAGCAAGTACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTg                                 <  5:379243/148‑1 (MQ=255)                                                                                                                           tCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCt                    >  6:212804/1‑148 (MQ=255)                                                                                                                             gCTTTCGCAACTTGAGCAAGTACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCttt                  >  7:218546/1‑148 (MQ=255)                                                                                                                                ttCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGttt                                                                  >  3:161433/1‑97 (MQ=255)                                                                                                                                ttCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGttt                                                                  <  4:161433/97‑1 (MQ=255)                                                                                                                                   gCAACTTGAGCAAGTACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCtt             <  8:218546/147‑1 (MQ=255)                                                                                                                                      aCTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGAt         <  3:184447/148‑1 (MQ=255)                                                                                                                                      aCTTGAGCAAGCACCACCGCAAGGTACGATGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGAt         <  5:366280/148‑1 (MQ=255)                                                                                                                                           agaaagcaCCACCGCAAGGTACGCTGGCCTCTTTATTACTTAATTACACCTCAGGCATAGCGTTCACCGCCACCTCCCTGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCa     <  7:198092/144‑1 (MQ=255)                                                                                                                                             caagcaCCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATcc  <  8:382426/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                GCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTGGCGGCTGTACTGCTCGCTTTCGCAACTTGAGCAAGCACCACCGCAAGGTACGCTGGCCTCTTAATTACTTAATTACACCACAGGCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCC  >  NC_000913/1723961‑1724248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑