Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,128,084 T→C 5.5% E77E (GAA→GAG)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,128,0840TC 5.5% 227.8 / 3.0 73E77E (GAA→GAG) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base C (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGCA  >  NC_000913/2127940‑2128229
                                                                                                                                                |                                                                                                                                                 
tttcttttcCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGAAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCa                                                                                                                                                >  5:280771/1‑148 (MQ=255)
 tttctttcCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCAc                                                                                                                                               >  7:187865/5‑148 (MQ=255)
       tcCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCaaa                                                                                                                                          <  1:345704/147‑1 (MQ=255)
             ccGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGcgcg                                                                                                                                   >  6:197642/1‑148 (MQ=255)
                aGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCa                                                                                                                                <  6:375302/148‑1 (MQ=255)
                  gAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAgg                                                                                                                              <  5:85814/148‑1 (MQ=255)
                    aGCGGATCGCCTCCTGCAGGCGCAGAGTACCCATCGCGGCGACGTCAGCGGTATTTTCTGGGGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGtt                                                                                                                            <  5:252940/148‑1 (MQ=255)
                    aGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGTTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGtt                                                                                                                            <  5:258651/148‑1 (MQ=255)
                     gCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTa                                                                                                                           <  5:197642/148‑1 (MQ=255)
                       ggATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTaga                                                                                                                         <  1:22659/148‑1 (MQ=255)
                       ggATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTaga                                                                                                                         <  7:332054/148‑1 (MQ=255)
                                 cagcagGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACt                                                                                                                <  7:66266/147‑1 (MQ=255)
                                   gcagGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAg                                                                                                             >  4:12476/1‑148 (MQ=255)
                                               tACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATg                                                                                                 >  4:48942/1‑148 (MQ=255)
                                                aCCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGt                                                                                                                      <  1:306719/126‑1 (MQ=255)
                                                aCCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGt                                                                                                                      >  2:306719/1‑126 (MQ=255)
                                                  ccATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAg                                                                                              <  5:254274/148‑1 (MQ=255)
                                                   cATCGCGTCGACGTCCGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATg                                                                                                 >  2:280322/1‑144 (MQ=255)
                                                      cgcgTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATg                                                                                           >  2:191956/1‑147 (MQ=255)
                                                            gACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAAttt                                                                                     <  3:184565/147‑1 (MQ=255)
                                                            gACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTc                                                                                    >  7:106957/1‑148 (MQ=255)
                                                                tCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGt                                                                                >  5:117543/1‑148 (MQ=255)
                                                                    cGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCa                                                                            <  1:280322/148‑1 (MQ=255)
                                                                    cGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCa                                                                            <  8:106957/148‑1 (MQ=255)
                                                                        atatTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGgtg                                                                        >  3:261098/1‑148 (MQ=255)
                                                                          atTCTGGTGACTCAAAAGCGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACCCTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTAAGGTCGCCAAAATGCAGATGGAATTTCGGGTAGCAGCtgtg                                                                      >  2:9048/1‑148 (MQ=255)
                                                                          atTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGtgtg                                                                      >  3:80296/1‑148 (MQ=255)
                                                                            tCTGGTGACTCAAAAGAGACCGCAACGTGTCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCggg                                                                                 >  6:263511/1‑135 (MQ=255)
                                                                            tCTGGTGACTCAAAAGAGACCGCAACGTGTCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCggg                                                                                 <  5:263511/135‑1 (MQ=255)
                                                                            tCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCg                                                                    >  2:227935/1‑148 (MQ=255)
                                                                                 tGACTCAAAAGAGACCGCAACGTGTCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCg                                                                                   <  7:217736/128‑1 (MQ=255)
                                                                                 tGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCg                                                                                   >  8:217736/1‑128 (MQ=255)
                                                                                 tGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATcc                                                               <  7:27716/148‑1 (MQ=255)
                                                                                 tGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACCTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATcc                                                               >  7:405201/1‑148 (MQ=255)
                                                                                 tGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACCTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATcc                                                               >  7:410783/1‑148 (MQ=255)
                                                                                    cTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGa                                                            >  2:401713/1‑148 (MQ=255)
                                                                                       aaaaGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATa                                                          <  4:80296/147‑1 (MQ=255)
                                                                                       aaaaGAGACCGCAACGTGGCGCATAGAGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCTGTTTGCAGGTTTGCTGATCGTGATaa                                                         <  4:151199/148‑1 (MQ=255)
                                                                                        aaaGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCTGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTAACaaa                                                        >  7:68504/1‑148 (MQ=255)
                                                                                        aaaGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATaaa                                                        >  1:374232/1‑148 (MQ=255)
                                                                                         aaGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAAt                                                       <  1:401713/148‑1 (MQ=255)
                                                                                         aaGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCAGAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAAt                                                       >  5:258308/1‑148 (MQ=255)
                                                                                            agaCCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAAtgtg                                                    <  3:12476/148‑1 (MQ=255)
                                                                                             gaCCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGa                                                   <  1:5136/148‑1 (MQ=255)
                                                                                             gaCCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGa                                                   <  3:48942/148‑1 (MQ=255)
                                                                                              aCCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGAt                                                  >  4:365275/1‑148 (MQ=255)
                                                                                                  cAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCAc                                              <  4:261098/148‑1 (MQ=255)
                                                                                                  cAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCAc                                              >  6:288242/1‑148 (MQ=255)
                                                                                                   aaCGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCAcg                                             <  3:99936/148‑1 (MQ=255)
                                                                                                       tGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCt                                         <  1:227935/148‑1 (MQ=255)
                                                                                                                 cgcCGAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTATAGGTATCACTCAGGGCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGaa                               <  6:334695/148‑1 (MQ=255)
                                                                                                                 cgcCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGa                                <  1:191956/147‑1 (MQ=255)
                                                                                                                 cgcCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGa                                <  5:288242/147‑1 (MQ=255)
                                                                                                                      aGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGAc                           >  5:48872/1‑147 (MQ=255)
                                                                                                                       ggTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTAGGGTTGCAGGTGTGCTGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGa                         >  5:336638/1‑148 (MQ=255)
                                                                                                                       gcttGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGa                         >  2:29412/3‑148 (MQ=255)
                                                                                                                              cacTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGa                  >  6:167601/1‑148 (MQ=255)
                                                                                                                              cacTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGGTAGAGGTATCACTCAGATCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGcgcg                   <  8:187865/147‑1 (MQ=255)
                                                                                                                               acTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGAc                 >  4:48867/1‑148 (MQ=255)
                                                                                                                                cTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACg                >  3:238511/1‑148 (MQ=255)
                                                                                                                                cTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACg                >  7:26357/1‑148 (MQ=255)
                                                                                                                                 ttCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGCGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGc               >  2:126055/1‑148 (MQ=255)
                                                                                                                                    aTCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTa            <  2:374232/148‑1 (MQ=255)
                                                                                                                                     tCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTAGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTaa           <  3:365275/148‑1 (MQ=255)
                                                                                                                                       cGGCTGTACCTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATa         <  8:405201/148‑1 (MQ=255)
                                                                                                                                       cGGCTGTACCTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATa         <  8:410783/148‑1 (MQ=255)
                                                                                                                                          cTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAAt          <  7:191317/144‑1 (MQ=255)
                                                                                                                                          cTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAAt          >  8:191317/1‑144 (MQ=255)
                                                                                                                                           tGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCAt     >  6:22604/1‑148 (MQ=255)
                                                                                                                                             tACTTCACGCAAAATGCGCGTCAGGTTAGAGGTCTCACTAAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGc   <  6:280771/148‑1 (MQ=255)
                                                                                                                                             tACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGc   >  4:60126/1‑148 (MQ=255)
                                                                                                                                              aCTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGCTCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGCa  >  5:45724/1‑148 (MQ=255)
                                                                                                                                              aCTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGAAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGCa  >  2:49616/1‑148 (MQ=255)
                                                                                                                                                ttCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGa                                >  5:214351/1‑116 (MQ=255)
                                                                                                                                                ttCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGa                                <  6:214351/116‑1 (MQ=255)
                                                                                                                                                ttCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGAc                 <  7:51390/131‑1 (MQ=255)
                                                                                                                                                ttCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGAc                 >  8:51390/1‑131 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGACGTCAGCGGTATATTCTGGTGACTCAAAAGAGACCGCAACGTGGCTCATTGCGCCCAGGTTGTACACTTCATCCGGCTGTACTTCACGCAAAATGCGCGTCAGGTTAGAGGTATCACTCAGGTCGCCATAATGCAGATGGAATTTCGGGTTGCAGGTGTGCGGATCCTGATAAATGTGATCCACGCGCTCGGTGTTGAATGACGATGCGCGACGCTTAATACCATGCA  >  NC_000913/2127940‑2128229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑