Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,306,444 T→A 5.3% K104I (AAA→ATA)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,306,4440TA 5.3% 250.9 / 3.1 75K104I (AAA→ATA) mqomalate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (33/38);  new base A (2/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATT  >  NC_000913/2306297‑2306588
                                                                                                                                                   |                                                                                                                                                
aGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtt                                                                                                                                                  <  7:330082/148‑1 (MQ=255)
  gAAATTGACGATACCCTCGCCCCAGACAACGCTCATATGCGGAACGGTAGTGATAATTGAACGCGGAGTACGCAGCACGCCGCCCTCAACCTGGTGCGCCCAGATCTGGCGGGAAAGCTGAAATGCGTCGTTAAAGGCGACAGCtttt                                                                                                                                                >  6:276687/1‑148 (MQ=255)
   aaaTTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCtttt                                                                                                                                                >  4:271113/1‑147 (MQ=255)
   aaaTTGACGTTATCCTCGCCCAAGACAAAGCTCATATGCGTAACGGTATTGATAAATTAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCGCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCttttt                                                                                                                                               <  4:250472/148‑1 (MQ=255)
    aaTTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTc                                                                                                                                              <  3:329965/148‑1 (MQ=255)
    aaTTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTc                                                                                                                                              <  4:163204/148‑1 (MQ=255)
    aaTTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTc                                                                                                                                              <  8:322891/148‑1 (MQ=255)
     aTTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTc                                                                                                                                              >  2:396813/1‑147 (MQ=255)
        gACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAAt                                                                                                                                           <  3:271113/147‑1 (MQ=255)
             tATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTATTTCAATACTGAt                                                                                                                                     >  7:196303/1‑148 (MQ=255)
             tATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTATTTCAATACTGAt                                                                                                                                     >  7:190428/1‑148 (MQ=255)
              aTCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAt                                                                                                                                     <  3:9376/147‑1 (MQ=255)
                 cTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAtgct                                                                                                                                  <  8:388568/147‑1 (MQ=255)
                   cGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAtgctgc                                                                                                                                >  2:284773/1‑147 (MQ=255)
                              aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGtttt                                                                                                                    <  5:88756/148‑1 (MQ=255)
                              aaGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGtttt                                                                                                                    >  6:222451/1‑148 (MQ=255)
                               aGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCTCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTg                                                                                                                   <  6:359592/148‑1 (MQ=255)
                                gCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGc                                                                                                                  <  1:284773/148‑1 (MQ=255)
                                    atatGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCgg                                                                                                                <  1:71322/146‑1 (MQ=255)
                                    atatGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCgg                                                                                                                <  2:217812/146‑1 (MQ=255)
                                    atatGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCgg                                                                                                                <  2:59719/146‑1 (MQ=255)
                                     tatGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgt                                                                                                             >  2:243129/1‑148 (MQ=255)
                                      atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTTGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTg                                                                                                                                       <  2:231036/121‑1 (MQ=255)
                                      atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTTGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTg                                                                                                                                       >  1:231036/1‑121 (MQ=255)
                                      atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg                                                                                                            >  6:357749/1‑148 (MQ=255)
                                      atGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtg                                                                                                            >  6:340957/1‑148 (MQ=255)
                                       tGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgtgt                                                                                                           <  1:168159/148‑1 (MQ=255)
                                        gCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgt                                                                                                             <  8:288473/145‑1 (MQ=255)
                                        gCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGgt                                                                                                             >  7:288473/1‑145 (MQ=255)
                                            aaCGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttc                                                                                                      <  5:222451/148‑1 (MQ=255)
                                                 tATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagttc                                                                                                 <  2:174576/148‑1 (MQ=255)
                                                 tATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTATTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagttc                                                                                                 <  8:196303/148‑1 (MQ=255)
                                                 tATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTATTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTagttcagttc                                                                                                 <  8:190428/148‑1 (MQ=255)
                                                    tGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCa                                                                                               >  8:79786/1‑147 (MQ=255)
                                                    tGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCa                                                                                               <  7:79786/147‑1 (MQ=255)
                                                      aTAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATc                                                                                             <  3:180458/147‑1 (MQ=255)
                                                             aaCGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGa                                                                                     <  1:396813/148‑1 (MQ=255)
                                                             aaCGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGa                                                                                     <  8:255017/148‑1 (MQ=255)
                                                              aCGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGaa                                                                                    >  2:381295/1‑148 (MQ=255)
                                                                 cgGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGc                                                                                 >  3:62270/1‑148 (MQ=255)
                                                                       aCGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGtt                                                                           >  2:173997/1‑148 (MQ=255)
                                                                          cagcaCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCg                                                                        >  7:328485/1‑148 (MQ=255)
                                                                          cagcaCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCg                                                                        >  4:117178/1‑148 (MQ=255)
                                                                            gcaCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGAAGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGc                                                                      >  6:377930/1‑148 (MQ=255)
                                                                             caCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGa                                                                                     <  5:233879/132‑1 (MQ=255)
                                                                             caCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGa                                                                                     >  6:233879/1‑132 (MQ=255)
                                                                              aCGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCACCAGTGCAGAATGCCCGGTTCCGGCGt                                                                    >  2:343921/1‑148 (MQ=255)
                                                                                gccgcGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTa                                                                  <  8:328485/148‑1 (MQ=255)
                                                                                 ccgcGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGACTGCCCGTTTCCGGCGTTAt                                                                 >  2:330274/1‑148 (MQ=255)
                                                                                 ccgcGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTAt                                                                 >  6:392735/1‑148 (MQ=255)
                                                                                 ccgcGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTAt                                                                 >  3:146067/1‑148 (MQ=255)
                                                                                 ccgcGCTCAACCTGGTGCACCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTAt                                                                 >  6:142395/1‑148 (MQ=255)
                                                                                    cgcTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTcc                                                              <  1:195776/148‑1 (MQ=255)
                                                                                     gcTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGTGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCa                                                             <  1:205393/148‑1 (MQ=255)
                                                                                      cTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAg                                                            >  2:208245/1‑148 (MQ=255)
                                                                                        cAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGcc                                                          >  6:249893/1‑148 (MQ=255)
                                                                                               gTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGaa                                                   <  1:173997/148‑1 (MQ=255)
                                                                                               gTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGa                                                    <  4:62270/147‑1 (MQ=255)
                                                                                                tGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAAc                                                  <  5:357749/148‑1 (MQ=255)
                                                                                                  cgcCCAGAACTGGCGGGAAATCTGAAATGGTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAActc                                                <  5:276687/148‑1 (MQ=255)
                                                                                                    cccAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACtctc                                              <  5:392735/148‑1 (MQ=255)
                                                                                                    cccAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACtctc                                              <  5:340957/148‑1 (MQ=255)
                                                                                                        gAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTgc                                          >  3:192830/1‑148 (MQ=255)
                                                                                                        gAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGCCGTTTTGAGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTgc                                          >  7:320869/1‑148 (MQ=255)
                                                                                                          aCTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTgcgc                                        >  1:58597/1‑148 (MQ=255)
                                                                                                             ggCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGac                                     >  6:333488/1‑148 (MQ=255)
                                                                                                             ggCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGa                                      >  4:163223/1‑147 (MQ=255)
                                                                                                                  gAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCt                                >  7:8560/1‑148 (MQ=255)
                                                                                                                   aaaTCTGAAATGCTTCGTTAATGGCGACTGCGTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGCTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTc                               <  5:360790/148‑1 (MQ=255)
                                                                                                                    aaTCTGAAATGCTTCGTTAATGGCGACTGCTTTTCCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTcc                              <  5:112634/148‑1 (MQ=255)
                                                                                                                         gAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTg                                                                                                                                       <  2:318989/38‑1 (MQ=255)
                                                                                                                         gAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTg                                                                                                                                       >  1:318989/1‑38 (MQ=255)
                                                                                                                          aaaTGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCAATCAGTGCAGAATGCCCGATTCCTGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCATCCAGtcgc                        >  1:82535/1‑148 (MQ=255)
                                                                                                                           aaTGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGTATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCt                       >  6:346615/1‑148 (MQ=255)
                                                                                                                           aaTGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACTCTCCAGGCGCt                       >  8:148829/1‑148 (MQ=255)
                                                                                                                               cTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTccac                   <  1:262523/148‑1 (MQ=255)
                                                                                                                                  cGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTccacc                  <  1:243129/146‑1 (MQ=255)
                                                                                                                                             aCTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGTATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGAcc     <  5:346615/148‑1 (MQ=255)
                                                                                                                                                 cTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATCGTCATCGCCCAtt  <  5:377930/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAAATCTGAAATGCTTCGTTAATGGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATT  >  NC_000913/2306297‑2306588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: