Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,351,872 C→T 8.0% G168D (GGT→GAT)  glpT ← sn‑glycerol‑3‑phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,351,8720CT 8.0% 150.4 / 2.9 50G168D (GGT→GAT) glpTsn‑glycerol‑3‑phosphate transporter
Reads supporting (aligned to +/- strand):  ref base C (20/26);  new base T (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

AGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAT  >  NC_000913/2351726‑2352017
                                                                                                                                                  |                                                                                                                                                 
aGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATaccaccacc                                                                                                                                                  <  1:104228/148‑1 (MQ=255)
 gCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCg                                                                                                                                                 >  6:99855/1‑148 (MQ=255)
    acaGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACg                                                                                                                                              >  5:161032/1‑148 (MQ=255)
     caGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGt                                                                                                                                             <  7:138148/148‑1 (MQ=255)
     caGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGt                                                                                                                                             <  3:188851/148‑1 (MQ=255)
     caGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGt                                                                                                                                             >  2:345683/1‑148 (MQ=255)
         aTTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgtg                                                                                                                                         <  1:345683/148‑1 (MQ=255)
         aTTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtgtg                                                                                                                                         <  6:161032/148‑1 (MQ=255)
                    tCGCGCATCATCGCAAAGGCGAATGATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                                                                                                                              >  8:119093/1‑148 (MQ=255)
                    tCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCGGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTcc                                                                                                                              <  3:268022/148‑1 (MQ=255)
                     cgcgCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGt                                                                                                                                             >  1:403300/1‑132 (MQ=255)
                     cgcgCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGt                                                                                                                                             <  2:403300/132‑1 (MQ=255)
                        gcATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcaccc                                                                                                                          >  3:294581/1‑146 (MQ=255)
                           tcatcGCTAAGGCGAATAATGCCACCAGAATGGCGGAGAAAGCAGGGATATAGAGCGCCGCATGCGAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGTAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGa                                                                                                                       <  4:364976/148‑1 (MQ=255)
                            catcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtg                                                                                                                                           <  4:308532/127‑1 (MQ=255)
                            catcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTtg                                                                                                                                           >  3:308532/1‑127 (MQ=255)
                             atcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGac                                                                                                                      <  2:110717/147‑1 (MQ=255)
                              tcGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGATCCACACTGaca                                                                                                                     >  3:140146/1‑147 (MQ=255)
                               cGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCCCCACCGACGTTGTGCGCACAGTTCCCCACTGacac                                                                                                                    >  5:182534/1‑147 (MQ=255)
                                gCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGAcaca                                                                                                                   <  8:4405/147‑1 (MQ=255)
                                       cGAATTATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATgccgcc                                                                                                           <  7:119093/148‑1 (MQ=255)
                                        gAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCa                                                                                                          <  5:99855/148‑1 (MQ=255)
                                            aatGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGtt                                                                                                      >  3:105372/1‑148 (MQ=255)
                                                ccaccaTTATTGCTCAGATATCAGGCATATAGAGCGCCGGATGAGAGTCATTGAACCTGTGCATCCTCAGCAGGATCAGCAGCGGCGGAATACCACGACCGTCGTTGTGCTCTCAGTTCCTCACTGACACAATGCCGCCACGttctt                                                                                                   <  4:51378/147‑1 (MQ=255)
                                                ccaccaGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGttctt                                                                                                   <  4:105372/147‑1 (MQ=255)
                                                                gAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGCACCAGCCCATCCCCAGCACCAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTACAACACTGACACAATGCCGCCACGTTCTTACTGCTACCACCAgag                                                                                   >  6:80078/1‑145 (MQ=255)
                                                                gAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGACCCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACTTTCTTTCTGCGACCACCAGTGc                                                                                  <  2:204313/148‑1 (MQ=255)
                                                                gAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTg                                                                                   >  1:200826/1‑147 (MQ=255)
                                                                  aaGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCAc                                                                                >  1:112164/1‑148 (MQ=255)
                                                                           tataGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACg                                                                       <  5:80078/148‑1 (MQ=255)
                                                                            ataGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAt                                                                             <  1:185187/141‑1 (MQ=255)
                                                                            ataGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCAt                                                                             >  2:185187/1‑141 (MQ=255)
                                                                            ataGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTa                                                                         >  6:213778/1‑145 (MQ=255)
                                                                            ataGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTa                                                                         <  5:213778/145‑1 (MQ=255)
                                                                             taGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcac                                                                                                                            >  6:313144/1‑93 (MQ=255)
                                                                             taGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCcac                                                                                                                            <  5:313144/93‑1 (MQ=255)
                                                                              agagCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGAc                                                                     >  6:60359/1‑147 (MQ=255)
                                                                               gagCGCCGCATGCCAGTCATTGAACCAGGCCTTCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGAcc                                                                    <  4:294581/147‑1 (MQ=255)
                                                                                     cgcATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACAcggc                                                             <  4:42581/148‑1 (MQ=255)
                                                                                      gcATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCATCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGacc                                                                                          >  2:248756/1‑118 (MQ=255)
                                                                                      gcATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCATCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGacc                                                                                          >  2:254625/1‑118 (MQ=255)
                                                                                      gcATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCATCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGacc                                                                                          <  1:254625/118‑1 (MQ=255)
                                                                                      gcATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCATCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGacc                                                                                          <  1:248756/118‑1 (MQ=255)
                                                                                         tGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGcc                                                         <  2:112164/148‑1 (MQ=255)
                                                                                            cAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACGAGTGCACCATAGTACGACCACACGGCGGCCAcc                                                      <  4:140146/148‑1 (MQ=255)
                                                                                              gTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCAccc                                                     >  5:8152/1‑147 (MQ=255)
                                                                                              gTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCAccc                                                     >  6:166458/1‑147 (MQ=255)
                                                                                                      aCCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTg                                            >  4:177428/1‑148 (MQ=255)
                                                                                                                   cagcagGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCag                               <  5:166458/148‑1 (MQ=255)
                                                                                                                            cagcagCGGCGTAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGaacaac                      <  6:8152/148‑1 (MQ=255)
                                                                                                                                  cggcggAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACa                <  5:60359/148‑1 (MQ=255)
                                                                                                                                           accaccaccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCAcc       >  1:130557/1‑148 (MQ=255)
                                                                                                                                                caccGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAt  <  2:200826/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCCACAGGATTGCGGGGTATCGCGCATCATCGCAAAGGCGAATAATGCCACCAGAATGGCGCAGAAAGCAGGCATATAGAGCGCCGCATGCCAGTCATTGAACCAGGCCATCCCCAGCAGGAACAGCAGCGGCGGAATACCACCACCGACGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAAT  >  NC_000913/2351726‑2352017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑