Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,680,316 C→T 5.3% P810P (CCG→CCA yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,680,3160CT 5.3% 244.8 / 2.2 75P810P (CCG→CCAyphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base C (34/37);  new base T (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

TGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTT  >  NC_000913/2680170‑2680461
                                                                                                                                                  |                                                                                                                                                 
tGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgcg                                                                                                                                                  >  4:317482/1‑148 (MQ=255)
  ccACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgcgg                                                                                                                                                 <  3:191108/147‑1 (MQ=255)
    acaAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgcggcg                                                                                                                                               >  4:157679/1‑147 (MQ=255)
     caAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGgcgc                                                                                                                                              >  2:383596/1‑147 (MQ=255)
     caAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGgcgc                                                                                                                                              >  7:397933/1‑147 (MQ=255)
       aaTTGAGCAGTTCTGCGGTCATGTCGTCACGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGCCGTGCATCCTGCGGCGCAAg                                                                                                                                           >  6:372901/1‑148 (MQ=255)
        aTTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATATCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGGTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGc                                                                                                                                          >  7:323302/1‑148 (MQ=255)
           gAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg                                                                                                                                        <  8:222571/147‑1 (MQ=255)
           gAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGGGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg                                                                                                                                        <  5:317634/147‑1 (MQ=255)
            aGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGa                                                                                                                                       <  3:79335/147‑1 (MQ=255)
                  tCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgcgg                                                                                                                                                 >  4:6247/1‑131 (MQ=255)
                  tCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgcgg                                                                                                                                                 <  3:6247/131‑1 (MQ=255)
                  tCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCAt                                                                                                                                >  1:333813/1‑148 (MQ=255)
                   cTGCGGTCATGTCGTCACGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCAt                                                                                                                                <  5:372901/147‑1 (MQ=255)
                       ggTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATc                                                                                                                            >  7:272895/1‑147 (MQ=255)
                       ggTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCa                                                                                                                           <  5:182672/148‑1 (MQ=255)
                       ggTCATGGCGGCGCGTTTCAGCTCATTTTCCTGTTTATTTTCCAGACGCGCCAGTCGTTTCTGCGGTGTGGGTCCAGTTAACTTATCCTGCAAATCCCGTTCGAAAAGCAGTCGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCa                                                                                                                           <  8:416904/148‑1 (MQ=255)
                        gTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg                                                                                                                                        >  5:6122/1‑134 (MQ=255)
                        gTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg                                                                                                                                        <  6:6122/134‑1 (MQ=255)
                         tCATGTCATCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATc                                                                                                                            >  1:362432/1‑145 (MQ=255)
                           aTGTCGTAGCGTTTCATCGCAATTTCCAGATTATTTTCCAGACTCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAATGCTTATCagctt                                                                                                                       <  3:328230/148‑5 (MQ=255)
                              tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAAc                                                                                                                    <  4:139539/148‑1 (MQ=255)
                                 tcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAAcgcg                                                                                                                 >  1:133050/1‑148 (MQ=255)
                                     gTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGc                                                                                                             >  8:371964/1‑148 (MQ=255)
                                      tttCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGc                                                                                                             >  2:34724/1‑147 (MQ=255)
                                       ttCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTgc                                                                                                                                                   <  5:186255/108‑1 (MQ=255)
                                       ttCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGc                                                                                                             >  6:227100/1‑146 (MQ=255)
                                        tCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCAGGTGTGGCTCAACTTAACTTATCCATCAAATCCCTTTCGAAAAGCAGACGTGCATCCTgcg                                                                                                                                                  >  6:186255/1‑108 (MQ=255)
                                              cAATTTCCAGATTATTTTCCAGACGCGCCAGTCGATTCTCCGGTCTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAACCACACGTGCAACCTGCGGAGCAAGCTGATAAGCATTATCAAGATACCGCGAGGCCAGCTCAt                                                                                                     >  2:5843/1‑147 (MQ=255)
                                                aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAAt                                                                                                  <  1:383596/148‑1 (MQ=255)
                                                   tCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCg                                                                                                <  1:8849/147‑1 (MQ=255)
                                                       gATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTg                                                                                           <  6:228908/148‑1 (MQ=255)
                                                         ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCt                                                                                         <  2:133050/148‑1 (MQ=255)
                                                           attTTCCAGACGCTCGAGTCGTTTCTCCGTTGTGGCTCCACTTAAGTTATCCATCATATCCCGTTCGAATAGGAGACGTGCATCATGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTAATAATCGTGTTGCtt                                                                                        <  1:5843/147‑1 (MQ=255)
                                                           attTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCtt                                                                                        <  8:397933/147‑1 (MQ=255)
                                                           attTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCtt                                                                                        <  3:128855/147‑1 (MQ=255)
                                                           attTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATACAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTa                                                                                       >  7:379677/1‑148 (MQ=255)
                                                                  aGACGCGCCAGTCGTTTCTCCGGTGTGGATCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGATCATAATCGTGATGCTTATTCCACg                                                                                <  3:213/148‑1 (MQ=255)
                                                                        gcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGa                                                                          <  1:34724/148‑1 (MQ=255)
                                                                        gcCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGa                                                                          <  2:333813/148‑1 (MQ=255)
                                                                         ccAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGAt                                                                         >  3:254306/1‑148 (MQ=255)
                                                                          cAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATc                                                                        <  3:317482/148‑1 (MQ=255)
                                                                           aGTCGTTTCTCCGTAGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGAAGTGCATCCTTCGGCGCAAGATGATAAGAATTATCAAGATAACGAGCGGCCAGATCATAATCGTGTTGCTTAATCCACTCATGGATCg                                                                       >  8:301809/1‑148 (MQ=255)
                                                                             tCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGc                                                                      >  5:234245/1‑147 (MQ=255)
                                                                             tCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGc                                                                      >  5:235621/1‑147 (MQ=255)
                                                                             tCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGc                                                                      >  8:78560/1‑147 (MQ=255)
                                                                                       ggTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATa                                                                                                    >  7:210735/1‑107 (MQ=255)
                                                                                       ggTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATa                                                                                                    <  8:210735/107‑1 (MQ=255)
                                                                                            ggCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAg                                                                                                                         <  4:374247/81‑1 (MQ=255)
                                                                                            ggCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAg                                                                                                                         >  3:374247/1‑81 (MQ=255)
                                                                                              cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTc                                                    <  7:78560/148‑1 (MQ=255)
                                                                                                  acttaacttaTCCAGCAAATCCCGTTCGAAATGCAGACGTGCATCCTGTGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCg                                                   >  8:398208/1‑145 (MQ=255)
                                                                                                  acttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGTGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCg                                                   <  7:398208/145‑1 (MQ=255)
                                                                                                  acttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGTGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCg                                                   >  4:301444/1‑145 (MQ=255)
                                                                                                  acttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGTGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCg                                                   <  3:301444/145‑1 (MQ=255)
                                                                                                   cttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGa                                                                          >  2:91547/1‑121 (MQ=255)
                                                                                                   cttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGa                                                                          <  1:91547/121‑1 (MQ=255)
                                                                                                   cttaacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCaa                                               <  7:371964/148‑1 (MQ=255)
                                                                                                     taacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCgtg                                                                                              >  3:124218/1‑99 (MQ=255)
                                                                                                     taacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCgtg                                                                                              <  4:124218/99‑1 (MQ=255)
                                                                                                     taacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTa                                                                    <  6:123113/125‑1 (MQ=255)
                                                                                                     taacttaTCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTa                                                                    >  5:123113/1‑125 (MQ=255)
                                                                                                            tCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTc                                                                                    >  2:106166/1‑102 (MQ=255)
                                                                                                            tCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTc                                                                                    <  1:106166/102‑1 (MQ=255)
                                                                                                            tCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGc                                       >  6:154599/1‑147 (MQ=255)
                                                                                                              cAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGa                                     >  1:274010/1‑147 (MQ=255)
                                                                                                              cAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGa                                     <  2:362432/147‑1 (MQ=255)
                                                                                                              cAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGAc                                    <  5:227100/148‑1 (MQ=255)
                                                                                                              cAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGAGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGAc                                    <  6:235621/148‑1 (MQ=255)
                                                                                                                gaaaaTCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCTCGGCCAGCTCATAATCCTGTTGCTTATTCCACGCATGGATCTCTAACCCGAGCCAGCCGTCGGCAAACTCCGACGACAt                                  >  2:154590/3‑148 (MQ=255)
                                                                                                                gCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAAcgc                                                                                                                  <  2:66082/68‑1 (MQ=255)
                                                                                                                gCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAAcgc                                                                                                                  >  1:66082/1‑68 (MQ=255)
                                                                                                                gCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACAt                                  >  7:178622/1‑148 (MQ=255)
                                                                                                                gCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACAt                                  >  8:63509/1‑148 (MQ=255)
                                                                                                                 cAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACAtc                                 <  7:301809/148‑1 (MQ=255)
                                                                                                                 cAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACAtc                                 <  3:157679/148‑1 (MQ=255)
                                                                                                                              aaaaGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGtt                    >  2:236635/1‑148 (MQ=255)
                                                                                                                                         tGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCa         <  8:272895/148‑1 (MQ=255)
                                                                                                                                         tGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCa         <  4:254306/148‑1 (MQ=255)
                                                                                                                                          gCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTcc                                          <  8:199242/114‑1 (MQ=255)
                                                                                                                                          gCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTcc                                          >  7:199242/1‑114 (MQ=255)
                                                                                                                                                tGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCtt  >  4:44802/1‑148 (MQ=255)
                                                                                                                                                  cggcgCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCtt  <  2:274010/146‑1 (MQ=255)
                                                                                                                                                  cggcgCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCtt  <  7:401239/146‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGCCACAAATTGAGCAGTTCTGCGGTCATGTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAGCATTATCAAGATAACGCGCGGCCAGCTCATAATCGTGTTGCTTATTCCACGCATGGATCGCTAACCCGCGCCAGCCGTCGGCAAACTCCGGCGACATCTCTACGCAACGTTGCCAAAAGGCAATGGCTT  >  NC_000913/2680170‑2680461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: