Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,142,784 G→T 5.6% A69E (GCG→GAG)  hybC ← hydrogenase 2, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,142,7840GT 5.6% 244.9 / 3.0 71A69E (GCG→GAG) hybChydrogenase 2, large subunit
Reads supporting (aligned to +/- strand):  ref base G (29/38);  new base T (2/2);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTT  >  NC_000913/3142639‑3142925
                                                                                                                                                 |                                                                                                                                             
cACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGcgcg                                                                                                                                             <  7:6204/148‑1 (MQ=255)
  cccAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt                                                                                                                                            >  5:151538/1‑147 (MQ=255)
  cccAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg                                                                                                                                           <  8:387625/148‑1 (MQ=255)
   ccAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGa                                                                                                                                          <  6:135773/148‑1 (MQ=255)
    cAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGcgcg                                                                                                                                             >  1:231001/1‑144 (MQ=255)
    cAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGcgcg                                                                                                                                             <  2:231001/144‑1 (MQ=255)
     aGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt                                                                                                                                        <  1:123013/148‑1 (MQ=255)
     aGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt                                                                                                                                        <  3:133618/148‑1 (MQ=255)
     aGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt                                                                                                                                        <  1:375570/148‑1 (MQ=255)
         cAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt                                                                                                                                     <  6:151538/147‑1 (MQ=255)
          aGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCTCGAACGGAAGACAGCGCGTGAGTGgtag                                                                                                                                   >  2:108511/1‑148 (MQ=255)
          aGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtag                                                                                                                                   >  2:272514/1‑148 (MQ=255)
            cgcCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagta                                                                                                                                 <  1:272514/148‑1 (MQ=255)
              ccGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa                                                                                                                               >  5:261225/1‑148 (MQ=255)
               cGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACAt                                                                                                                              <  5:299242/148‑1 (MQ=255)
                  aaGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGa                                                                                                                                          >  8:199662/1‑133 (MQ=255)
                  aaGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGa                                                                                                                                          <  7:199662/133‑1 (MQ=255)
                     cTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCca                                                                                                                        >  7:102224/1‑148 (MQ=255)
                     cTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCca                                                                                                                        >  7:121984/1‑148 (MQ=255)
                        aTAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTTGTAGTACATACGCcac                                                                                                                       <  2:41843/146‑1 (MQ=255)
                        aTAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAg                                                                                                                     <  2:116061/148‑1 (MQ=255)
                             aaTGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAAGGATGTTACGGATGTATTGCGAGTTAACCTTAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACCGAAGACCGCGCGTGAGTGGTAGTACATACGCCACAGATACg                                                                                                                >  6:295112/1‑148 (MQ=255)
                              aTGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGt                                                                                                               <  8:102224/148‑1 (MQ=255)
                                gAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCTTg                                                                                                                                           <  4:342999/118‑1 (MQ=255)
                                gAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg                                                                                                                                           >  3:342999/1‑118 (MQ=255)
                                 aaCTATATGGTCATGCGTTGTTTGCGCAGCGATATTGATGTTAAGGATGTTTTGCTAGTTAACCGTAACGTAGATATTCTGCGAATTTTCTGCCGCACGAACGGAAGAGAGGGCGTGAGTTTTAGTATATACTCCACAGATACttttc                                                                                                            <  4:46075/148‑3 (MQ=255)
                                  aCAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGca                                                                                                           >  1:12353/1‑148 (MQ=255)
                                     atatTGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACaa                                                                                                        <  1:198756/148‑1 (MQ=255)
                                      tatGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAat                                                                                                       >  7:339590/1‑148 (MQ=255)
                                       atGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAat                                                                                                       >  7:384816/1‑147 (MQ=255)
                                           tCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATcc                                                                                                  <  7:314533/148‑1 (MQ=255)
                                             aTGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCa                                                                                                 <  8:384816/147‑1 (MQ=255)
                                              tGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATg                                                                                               >  6:144815/1‑148 (MQ=255)
                                                       tgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATcgcgcg                                                                                      >  2:238144/1‑148 (MQ=255)
                                                              ccAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATcacg                                                                               >  5:291161/1‑148 (MQ=255)
                                                                   atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGtttt                                                                          >  6:185763/1‑148 (MQ=255)
                                                                      atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTc                                                                        <  1:238144/147‑1 (MQ=255)
                                                                        gTTACGGATGTATTGATCGTTAACCGGAAGGTCGATATTGAGCGCACTTTCTGCGGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACATTCTTCCATGCATCGCGCGGATCGCGGTTTTTCag                                                                      <  4:291541/147‑2 (MQ=255)
                                                                         ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGa                                                                    <  2:12353/148‑1 (MQ=255)
                                                                            cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtct                                                                 <  7:310373/148‑1 (MQ=255)
                                                                              gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtctc                                                                >  3:2020/1‑147 (MQ=255)
                                                                              gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtctc                                                                <  4:2020/147‑1 (MQ=255)
                                                                               aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                              <  1:326191/148‑1 (MQ=255)
                                                                               aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                              <  5:185763/148‑1 (MQ=255)
                                                                               aTGTATTGCGCGTTAACCGGAACGTCGATACTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGGGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                              <  8:202312/148‑1 (MQ=255)
                                                                                  tATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                            <  5:144815/147‑1 (MQ=255)
                                                                                  tATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                            <  8:339590/147‑1 (MQ=255)
                                                                                           ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGcc                                                  <  7:222973/148‑1 (MQ=255)
                                                                                            tAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCca                                                 >  8:201226/1‑148 (MQ=255)
                                                                                                cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                             >  8:123424/1‑148 (MQ=255)
                                                                                                cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                             >  8:117448/1‑148 (MQ=255)
                                                                                                cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                             >  1:178052/1‑148 (MQ=255)
                                                                                                   aaCGTCGATATTCAGCGCACTTTCTGCCTCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCAGAGATACGTTGCACAATCATACATGAATCGCGCGGATCGCGGTTTTTCTCGATCTCTTCCATGCAGCGCCACATCGTa                                          >  3:191513/1‑148 (MQ=255)
                                                                                                        cGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                              >  8:382452/1‑139 (MQ=255)
                                                                                                        cGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                              <  7:382452/139‑1 (MQ=255)
                                                                                                         gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGa                                     <  7:201226/147‑1 (MQ=255)
                                                                                                         gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCACGGTTTTTCACGATCTCTTCCAAGCCGCGCCACATGGTACCGGaa                                    <  6:291161/148‑1 (MQ=255)
                                                                                                         gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGGTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGa                                     <  1:233992/147‑1 (MQ=255)
                                                                                                           tatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                              >  5:383853/1‑136 (MQ=255)
                                                                                                           tatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                              <  6:383853/136‑1 (MQ=255)
                                                                                                              tCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                            >  4:53864/1‑119 (MQ=255)
                                                                                                              tCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                            <  3:53864/119‑1 (MQ=255)
                                                                                                                aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCTCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCAt                              <  6:261225/147‑1 (MQ=255)
                                                                                                                        ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGcc                                 >  3:281681/1‑136 (MQ=255)
                                                                                                                        ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGcc                                 <  4:281681/136‑1 (MQ=255)
                                                                                                                        ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGAAACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGACCATGCTTTCGa                      >  2:350405/1‑147 (MQ=255)
                                                                                                                          cTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGaaa                    <  1:155939/147‑1 (MQ=255)
                                                                                                                            gCCGCACGAACGGAAGACAGCGCGTGAGAGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAacga                 >  3:38044/1‑148 (MQ=255)
                                                                                                                                 aCGAACGGAAGACAGCGCGTGAGTGGTAGTATATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGCCGCCa            >  3:128444/1‑148 (MQ=255)
                                                                                                                                    aaCGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt         >  4:168913/1‑148 (MQ=255)
                                                                                                                                    aaCGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt         >  6:323272/1‑148 (MQ=255)
                                                                                                                                        gAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCTGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGa     <  8:121984/148‑1 (MQ=255)
                                                                                                                                           gACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAttt  <  7:123424/148‑1 (MQ=255)
                                                                                                                                           gACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAttt  <  7:117448/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTT  >  NC_000913/3142639‑3142925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: