Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,142,835 T→C 5.2% D52G (GAT→GGT)  hybC ← hydrogenase 2, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,142,8350TC 5.2% 239.7 / 2.2 76D52G (GAT→GGT) hybChydrogenase 2, large subunit
Reads supporting (aligned to +/- strand):  ref base T (36/36);  new base C (2/2);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCT  >  NC_000913/3142694‑3142982
                                                                                                                                             |                                                                                                                                                   
tgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATcgcgcg                                                                                                                                               >  2:238144/1‑148 (MQ=255)
       ccAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATcacg                                                                                                                                        >  5:291161/1‑148 (MQ=255)
            atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGtttt                                                                                                                                   >  6:185763/1‑148 (MQ=255)
               atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTc                                                                                                                                 <  1:238144/147‑1 (MQ=255)
                 gTTACGGATGTATTGATCGTTAACCGGAAGGTCGATATTGAGCGCACTTTCTGCGGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACATTCTTCCATGCATCGCGCGGATCGCGGTTTTTCag                                                                                                                               <  4:291541/147‑2 (MQ=255)
                  ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGa                                                                                                                             <  2:12353/148‑1 (MQ=255)
                     cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtct                                                                                                                          <  7:310373/148‑1 (MQ=255)
                       gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtctc                                                                                                                         >  3:2020/1‑147 (MQ=255)
                       gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGAtctc                                                                                                                         <  4:2020/147‑1 (MQ=255)
                        aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                                                                                       <  1:326191/148‑1 (MQ=255)
                        aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                                                                                       <  5:185763/148‑1 (MQ=255)
                        aTGTATTGCGCGTTAACCGGAACGTCGATACTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGGGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCtt                                                                                                                       <  8:202312/148‑1 (MQ=255)
                           tATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                                                                                     <  8:339590/147‑1 (MQ=255)
                           tATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                                                                                     <  5:144815/147‑1 (MQ=255)
                                    ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGcc                                                                                                           <  7:222973/148‑1 (MQ=255)
                                     tAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCca                                                                                                          >  8:201226/1‑148 (MQ=255)
                                         cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                                                                                      >  8:123424/1‑148 (MQ=255)
                                         cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                                                                                      >  8:117448/1‑148 (MQ=255)
                                         cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATg                                                                                                      >  1:178052/1‑148 (MQ=255)
                                            aaCGTCGATATTCAGCGCACTTTCTGCCTCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCAGAGATACGTTGCACAATCATACATGAATCGCGCGGATCGCGGTTTTTCTCGATCTCTTCCATGCAGCGCCACATCGTa                                                                                                   >  3:191513/1‑148 (MQ=255)
                                                 cGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                                                                                       >  8:382452/1‑139 (MQ=255)
                                                 cGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                                                                                       <  7:382452/139‑1 (MQ=255)
                                                  gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGa                                                                                              <  7:201226/147‑1 (MQ=255)
                                                  gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCACGGTTTTTCACGATCTCTTCCAAGCCGCGCCACATGGTACCGGaa                                                                                             <  6:291161/148‑1 (MQ=255)
                                                  gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGGTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGa                                                                                              <  1:233992/147‑1 (MQ=255)
                                                    tatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                                                                                       >  5:383853/1‑136 (MQ=255)
                                                    tatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACAt                                                                                                       <  6:383853/136‑1 (MQ=255)
                                                       tCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                                                                                     <  3:53864/119‑1 (MQ=255)
                                                       tCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTcc                                                                                                                     >  4:53864/1‑119 (MQ=255)
                                                         aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCTCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCAt                                                                                       <  6:261225/147‑1 (MQ=255)
                                                                 ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGcc                                                                                          <  4:281681/136‑1 (MQ=255)
                                                                 ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGcc                                                                                          >  3:281681/1‑136 (MQ=255)
                                                                 ttCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGAAACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGACCATGCTTTCGa                                                                               >  2:350405/1‑147 (MQ=255)
                                                                   cTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGaaa                                                                             <  1:155939/147‑1 (MQ=255)
                                                                     gCCGCACGAACGGAAGACAGCGCGTGAGAGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAacga                                                                          >  3:38044/1‑148 (MQ=255)
                                                                          aCGAACGGAAGACAGCGCGTGAGTGGTAGTATATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGCCGCCa                                                                     >  3:128444/1‑148 (MQ=255)
                                                                             aaCGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt                                                                  >  4:168913/1‑148 (MQ=255)
                                                                             aaCGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt                                                                  >  6:323272/1‑148 (MQ=255)
                                                                                 gAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCTGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGa                                                              <  8:121984/148‑1 (MQ=255)
                                                                                    gACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAttt                                                           <  7:123424/148‑1 (MQ=255)
                                                                                    gACAGCTCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCACCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAttt                                                           <  7:117448/148‑1 (MQ=255)
                                                                                            gTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCg                                                   <  1:108511/148‑1 (MQ=255)
                                                                                               aGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGAt                                                 <  2:178052/147‑1 (MQ=255)
                                                                                                    tagtaCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTaa                                           <  4:183806/148‑1 (MQ=255)
                                                                                                      gtaCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCaa                                                      >  3:76830/1‑135 (MQ=255)
                                                                                                      gtaCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCaa                                                      <  4:76830/135‑1 (MQ=255)
                                                                                                       taCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTa                                                                                                   <  7:173481/89‑1 (MQ=255)
                                                                                                       taCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTa                                                                                                   >  8:173481/1‑89 (MQ=255)
                                                                                                       taCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt                                                                  <  5:311977/122‑1 (MQ=255)
                                                                                                       taCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCAttt                                                                  >  6:311977/1‑122 (MQ=255)
                                                                                                        aaaTACGCCACAGATACGTTGAACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCGCATGCTTTCGAAAAGAAGCCATTTTCGATTTAGCAATCGATGCTTAAATg                                        >  6:382368/3‑147 (MQ=255)
                                                                                                        aCATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGCAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGGAAATGc                                       >  7:312095/1‑148 (MQ=255)
                                                                                                        aCATACGCCACAGATACGTTGCACAATCATCCATGAATCGCGCGGATCGCGGTTTTTCACGATCTCTGCCATGCCGCGCACCATGGTTCCGGCAGCCCATGCTTTCGCAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGc                                       >  2:317807/1‑148 (MQ=255)
                                                                                                          aTACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCg                                                   >  6:375424/1‑134 (MQ=255)
                                                                                                          aTACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCg                                                   <  5:375424/134‑1 (MQ=255)
                                                                                                          aTACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGccc                                     >  5:128354/1‑148 (MQ=255)
                                                                                                            aCGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGaa                                                                              >  1:405553/1‑105 (MQ=255)
                                                                                                            aCGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGaa                                                                              <  2:405553/105‑1 (MQ=255)
                                                                                                            aCGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAt                                                             >  1:263874/1‑122 (MQ=255)
                                                                                                            aCGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGAt                                                             <  2:263874/122‑1 (MQ=255)
                                                                                                                cacaGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCaa                                >  3:46942/1‑147 (MQ=255)
                                                                                                                cacaGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCAATGCAGCGCCACATGGTACCCGACGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGAAATCCATGCGTAAATGCCCCACaa                                >  1:280342/1‑147 (MQ=255)
                                                                                                                   aGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACg                            >  4:370895/1‑148 (MQ=255)
                                                                                                                    gATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAACCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACgg                           >  8:296394/1‑148 (MQ=255)
                                                                                                                       aCGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGt                         >  5:150499/1‑147 (MQ=255)
                                                                                                                                aaTCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCa               <  3:370895/148‑1 (MQ=255)
                                                                                                                                  tcatcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAAt             <  7:296394/148‑1 (MQ=255)
                                                                                                                                   catcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCg                                                   >  5:253089/1‑109 (MQ=255)
                                                                                                                                   catcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCg                                                   <  6:253089/109‑1 (MQ=255)
                                                                                                                                   catcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTaa                                           <  1:120838/117‑1 (MQ=255)
                                                                                                                                   catcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTaa                                           >  2:120838/1‑117 (MQ=255)
                                                                                                                                   catcCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCaa              >  8:411404/1‑146 (MQ=255)
                                                                                                                                     tcCATGCATCGCGCGCATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACTACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGt          <  5:382368/148‑1 (MQ=255)
                                                                                                                                        aTGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATa            >  6:203349/1‑143 (MQ=255)
                                                                                                                                        aTGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATa            <  5:203349/143‑1 (MQ=255)
                                                                                                                                          gCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCTGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATtc     >  8:383644/1‑148 (MQ=255)
                                                                                                                                             tCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTctct  <  7:411404/148‑1 (MQ=255)
                                                                                                                                             tCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGAAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTctct  >  2:178174/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGCGGTTTTTCACGATCTCTTCCATGCCGCGCCACATGGTACCGGAAGCCCATGCTTTCGAAACGACGCCATTTTCGATTTCGCAATCGATGCGTAAATGCCCCTCAATACGGGTTACCGGATCAATAGTAATTCTCT  >  NC_000913/3142694‑3142982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: