Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,254,732 C→G 6.0% G138G (GGC→GGG)  yhaK → redox‑sensitive bicupin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,254,7320CG 6.0% 230.7 / 5.8 67G138G (GGC→GGG) yhaKredox‑sensitive bicupin
Reads supporting (aligned to +/- strand):  ref base C (35/28);  new base G (2/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

TTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCA  >  NC_000913/3254589‑3254878
                                                                                                                                               |                                                                                                                                                  
ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAagc                                                                                                                                                >  4:180565/1‑148 (MQ=255)
ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAagc                                                                                                                                                <  3:110357/148‑1 (MQ=255)
 tgctgcTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAag                                                                                                                                                 >  6:349514/1‑146 (MQ=255)
      ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAgcagca                                                                                                                                            <  3:35208/146‑1 (MQ=255)
      ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAAt                                                                                                                                          >  7:250856/1‑148 (MQ=255)
            aCCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAAtcg                                                                                                                                    >  5:91190/1‑148 (MQ=255)
               cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAAtcgcct                                                                                                                                 <  7:410438/148‑1 (MQ=255)
               cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAAtcgcct                                                                                                                                 >  4:34825/1‑148 (MQ=255)
               cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAAtcgcct                                                                                                                                 <  1:97261/148‑1 (MQ=255)
                  ccGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAAtcgcctcgc                                                                                                                              <  3:34825/148‑1 (MQ=255)
                      gtgtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCa                                                                                                                            >  4:317688/1‑146 (MQ=255)
                       tgtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCagag                                                                                                                         <  1:166978/148‑1 (MQ=255)
                             cTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGc                                                                                                                    >  3:223704/1‑147 (MQ=255)
                                taGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGAt                                                                                                                 <  7:90243/147‑1 (MQ=255)
                                      acacAATCTCAGCAAAGACAACCCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGc                                                                                                          >  7:140389/1‑148 (MQ=255)
                                      acacAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGc                                                                                                          >  4:109281/1‑148 (MQ=255)
                                           aTCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCg                                                                                                                   >  7:152343/1‑134 (MQ=255)
                                           aTCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCg                                                                                                                   <  8:152343/134‑1 (MQ=255)
                                            tctcAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATggg                                                                                                              >  3:17911/1‑138 (MQ=255)
                                            tctcAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATggg                                                                                                              <  4:17911/138‑1 (MQ=255)
                                                aGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAAtt                                                                                                  <  6:91190/146‑1 (MQ=255)
                                                       aCAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCa                                                                                         <  3:317688/148‑1 (MQ=255)
                                                          aaCCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAgt                                                                                      >  3:331492/1‑148 (MQ=255)
                                                            ccGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAgtgt                                                                                    <  8:368054/148‑1 (MQ=255)
                                                             cGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGtgtg                                                                                   <  5:349514/148‑1 (MQ=255)
                                                                     cgAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATAACGCCAGCAAGTGTGGCTGCAc                                                                            >  5:251457/1‑147 (MQ=255)
                                                                      gAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCGCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCa                                                                          <  7:340487/148‑1 (MQ=255)
                                                                           cAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCg                                                                     >  6:304999/1‑148 (MQ=255)
                                                                            aGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGt                                                                    >  4:231679/1‑148 (MQ=255)
                                                                              cTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGc                                                                  <  5:304999/148‑1 (MQ=255)
                                                                              cTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGc                                                                  >  6:213015/1‑148 (MQ=255)
                                                                                 tGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCt                                                                 <  3:180565/146‑1 (MQ=255)
                                                                                 tGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCg                                                               >  6:323643/1‑148 (MQ=255)
                                                                                   gCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGa                                                              <  3:231679/147‑1 (MQ=255)
                                                                                    cTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGAc                                                             >  8:127449/1‑147 (MQ=255)
                                                                                        aCGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAgg                                                        >  8:305289/1‑148 (MQ=255)
                                                                                            cTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGGAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGa                                                     >  2:123203/1‑147 (MQ=255)
                                                                                            cTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGGAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGa                                                     >  2:117801/1‑147 (MQ=255)
                                                                                            cTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGaa                                                    <  7:127449/148‑1 (MQ=255)
                                                                                              gCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGACGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAg                                                  >  4:240279/1‑148 (MQ=255)
                                                                                               cccGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGaaa                                                   <  8:250856/146‑1 (MQ=255)
                                                                                               cccGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGt                                                 <  4:331492/148‑1 (MQ=255)
                                                                                                   cAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGa                                             <  3:109281/148‑1 (MQ=255)
                                                                                                   cAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCACCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCGCGACAAAGGCGAAAGTGCg                                              >  6:16223/1‑147 (MQ=255)
                                                                                                       gagagaATCCGCTGATTCAAAAGCTGGAGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAAttt                                         >  2:181013/1‑148 (MQ=255)
                                                                                                             aTCCGCTGATTCAAAAGCTGGCGCTTAATATGGGGAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCatt                                    <  1:117801/147‑3 (MQ=255)
                                                                                                             aTCCGCTGATTCAAAAGCTGGCGCTTAATATGGGGAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGt                                    <  1:123203/147‑1 (MQ=255)
                                                                                                              tCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTg                                  >  7:355351/1‑148 (MQ=255)
                                                                                                               ccGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCAAGCAAGTGTGGCTGCACCATATCGTGCTCGCCAAAGGCGAAAGTGCGAATTTCCAGTTg                                  >  6:97338/1‑147 (MQ=255)
                                                                                                                cGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGc                                 <  1:275543/147‑1 (MQ=255)
                                                                                                                cGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGc                                 <  7:305289/147‑1 (MQ=255)
                                                                                                                cGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGc                                 <  4:223704/147‑1 (MQ=255)
                                                                                                                cGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGc                                 <  2:381322/147‑1 (MQ=255)
                                                                                                                  cTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATg                              >  6:272853/1‑148 (MQ=255)
                                                                                                                      ttCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCCGCAAGTGTGGATGCACAATCACGTGAACGACAAAGGCGAAAGTGCGAATTTCAAGTTGCATGGGc                           >  6:214006/1‑147 (MQ=255)
                                                                                                                      ttCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGcc                          >  6:377816/1‑148 (MQ=255)
                                                                                                                          aaaGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCAcgc                      >  1:59843/1‑148 (MQ=255)
                                                                                                                               tGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTa                 >  1:212665/1‑148 (MQ=255)
                                                                                                                                  cgcTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTAttt              <  5:313995/148‑1 (MQ=255)
                                                                                                                                        atatGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATc        >  6:17645/1‑148 (MQ=255)
                                                                                                                                        atatGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATc        >  6:12408/1‑148 (MQ=255)
                                                                                                                                          atGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGa      >  6:360338/1‑148 (MQ=255)
                                                                                                                                          atGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGa      >  2:246465/1‑148 (MQ=255)
                                                                                                                                             ggCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGAtt    >  8:383039/1‑147 (MQ=255)
                                                                                                                                              gCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCa  >  1:372834/1‑148 (MQ=255)
                                                                                                                                              gCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCa  <  5:377816/148‑1 (MQ=255)
                                                                                                                                              gCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGACAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCa  <  5:323643/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTGCCCGCAGCGAGAGAATCCGCTGATTCAAAAGCTGGCGCTTAATATGGGCAAGCAGCAATTAATCGCCTCGCCAGAGGGTGCGATGGGAAGCCTGCAATTACGCCAGCAAGTGTGGCTGCACCATATCGTGCTCGACAAAGGCGAAAGTGCGAATTTCCAGTTGCATGGGCCACGCGCGTATTTGCAATCGATTCA  >  NC_000913/3254589‑3254878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑