Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,560,815 A→G 7.0% L213P (CTA→CCA)  glpG ← rhomboid intramembrane serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,8150AG 7.0% 168.0 / 3.5 57L213P (CTA→CCA) glpGrhomboid intramembrane serine protease
Reads supporting (aligned to +/- strand):  ref base A (27/26);  new base G (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCC  >  NC_000913/3560673‑3560958
                                                                                                                                              |                                                                                                                                               
cACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTGGCCAg                                                                                                                                            >  7:396470/1‑148 (MQ=255)
cACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTGGCCAg                                                                                                                                            >  7:402077/1‑148 (MQ=255)
cACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGc                                                                                                                                               >  5:116756/1‑145 (MQ=255)
cACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAg                                                                                                                                            <  8:236510/148‑1 (MQ=255)
cACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGAGAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGc                                                                                                                                               >  1:54919/1‑145 (MQ=255)
 aCGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGa                                                                                                                                           <  6:129992/148‑1 (MQ=255)
                 tgtgtgCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAgcgc                                                                                                                           <  5:321528/148‑1 (MQ=255)
                     tgCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATac                                                                                                                       <  1:207381/148‑1 (MQ=255)
                          cGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTGGCCAGACGTAGCCCATCAGCGCATAcacca                                                                                                                   <  8:402077/147‑1 (MQ=255)
                          cGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTGGCCAGACGTAGCCCATCAGCGCATAcacca                                                                                                                   <  8:396470/147‑1 (MQ=255)
                          cGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATaca                                                                                                                      >  4:217530/1‑144 (MQ=255)
                          cGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATaca                                                                                                                      <  3:217530/144‑1 (MQ=255)
                          cGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATAcacc                                                                                                                    <  5:384911/146‑1 (MQ=255)
                                ccATCGACATCCCAAACCAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATACTTAACCCACGTTGCAGGTAAATGCCACTTTGCGGAGCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGa                                                                                                            <  8:375482/148‑1 (MQ=255)
                                     gACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGAGGTAGCCCATCAGCGCATACACCACGCCAGAAAGcc                                                                                                       <  4:154852/148‑1 (MQ=255)
                                            caaacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCaaa                                                                                                >  3:259686/1‑148 (MQ=255)
                                              aacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAAcc                                                                                              <  1:166027/148‑1 (MQ=255)
                                              aacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAAc                                                                                               >  8:159198/1‑147 (MQ=255)
                                              aacaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAAc                                                                                               >  6:39164/1‑147 (MQ=255)
                                               acaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCa                                                                                             >  1:125019/1‑148 (MQ=255)
                                               acaaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCa                                                                                             <  2:125019/148‑1 (MQ=255)
                                                caaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGcc                                                                                                   >  8:351041/1‑141 (MQ=255)
                                                caaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGcc                                                                                                   <  7:351041/141‑1 (MQ=255)
                                                 aaaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATac                                                                                                                       <  7:420092/120‑1 (MQ=255)
                                                  aaTCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCTCGTTCGCCACGTAGACAGACGTAGCCCATCAGCGCATaaa                                                                                                                      >  8:420092/1‑118 (MQ=255)
                                                       aaCCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGc                                                                                     <  6:116756/148‑1 (MQ=255)
                                                        aCCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCt                                                                                    >  5:69923/1‑148 (MQ=255)
                                                                  gACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTtgc                                                                          <  2:354550/148‑1 (MQ=255)
                                                                    cAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATTCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAAtttt                                                                             <  3:277133/143‑1 (MQ=255)
                                                                    cAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAAtttt                                                                             >  4:277133/1‑143 (MQ=255)
                                                                         cAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGcaca                                                                    <  7:159198/147‑1 (MQ=255)
                                                                            aTCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGa                                                                                                            >  1:10506/1‑104 (MQ=255)
                                                                            aTCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGa                                                                                                            <  2:10506/104‑1 (MQ=255)
                                                                             tCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCACGCCCAACCACGGCCAGCTGAATTTTTGCTGCACATAGcc                                                               >  5:135264/1‑148 (MQ=255)
                                                                               aGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGc                                                             <  5:39164/148‑1 (MQ=255)
                                                                                   cAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCCCGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTaa                                                         >  3:367287/1‑148 (MQ=255)
                                                                                    aaaGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCa                                                                                                                          <  4:243210/82‑1 (MQ=255)
                                                                                    aaaGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCa                                                                                                                          >  3:243210/1‑82 (MQ=255)
                                                                                          aaTTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGgcg                                                  >  8:3808/1‑148 (MQ=255)
                                                                                                cccACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATa                                            <  4:259686/148‑1 (MQ=255)
                                                                                                   aCGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACGGGGCGCTGATAAGc                                         >  7:264721/1‑148 (MQ=255)
                                                                                                       tGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCCCATAGCCGCTTAACAGGGCGCTGATAAGCGTaa                                     >  5:24415/1‑148 (MQ=255)
                                                                                                           ggTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCg                                                              <  4:97761/119‑1 (MQ=255)
                                                                                                           ggTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCg                                                              >  3:97761/1‑119 (MQ=255)
                                                                                                            gTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGAc                                 >  2:19040/1‑147 (MQ=255)
                                                                                                                   ccACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCt                         >  7:45434/1‑148 (MQ=255)
                                                                                                                     aCTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTa                       <  6:69923/148‑1 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCCTCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCtt                        >  4:126547/1‑144 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAg                                                                                                                              >  6:42315/1‑42 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAAc                                                        >  4:342041/1‑112 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAAc                                                        <  3:342041/112‑1 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCtt                        <  3:126547/144‑1 (MQ=255)
                                                                                                                        ttGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCg                    >  1:234323/1‑148 (MQ=255)
                                                                                                                        ttGCGGATCGCGGTCGCCACGTAGCCAGACGTAGCCCATCAg                                                                                                                              <  5:42315/42‑1 (MQ=255)
                                                                                                                        atGCGGATCGCGTTCGCCACGTACCCATAAGTAGACCATCAGCGCAAACACCCCGACAGAAAGCCCGCCAAACCACGGCCCGCTCAATTATAGCTGCACATAGCCGCTTAACATCGCGCTGATAACCATAATGACAATTACCTTAcag                    >  8:293889/2‑146 (MQ=255)
                                                                                                                                     tCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGtt       >  7:149881/1‑148 (MQ=255)
                                                                                                                                          aCGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTcc  <  8:45434/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATCAAACCATCCGGCGACAATCCAGATCAGCGCAAAGATAATTAACCCACGTTGCAGGTAAATGCCACTTTGCGGATCGCGTTCGCCACGTAGCCAGACGTAGCCCATCAGCGCATACACCACGCCAGAAAGCCCGCCAAACCACGGCCCGCTGAATTTTTGCTGCACATAGCCGCTTAACAGGGCGCTGATAAGCGTAATGACAATTAGCTTACCGCTACCGAGGCGTTTTTCC  >  NC_000913/3560673‑3560958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑