Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,032	IS1 (–) +8 bp	intergenic (‑110/‑488)	hns ← / → tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	17 (0.530)	15/258	1.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 1293039	0 (0.000)					intergenic (‑117/‑488)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
*	?	NC_000913	1293032 =	0 (0.000)	30 (0.940)	29/258	0.2	100%	intergenic (‑110/‑495)	hns/tdk	global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
	?	NC_000913	= 1979270	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

GTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGAC  >  NC_000913/1292885‑1293179                                                                                                                                                    |                                                                                                                                                    gTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAtttt                                                                                                                                                    >  4:45074/1‑149 (MQ=255)             ttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt                                                                                                                                                     >  8:83724/1‑136 (MQ=255)             ttCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTAttt                                                                                                                                                     <  7:83724/136‑1 (MQ=255)                       tAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaac                                                                                                                               >  2:71494‑M2/1‑133 (MQ=255)                          gTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttat                                                                                                                           >  3:45074‑M2/1‑130 (MQ=255)                            gCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgcc                                                                                                                                               <  1:52220/127‑1 (MQ=255)                            gCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTgcc                                                                                                                                               >  2:52220/1‑127 (MQ=255)                                cgttacgcaccaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAAt                                                                                                                      <  5:107955‑M1/32‑1 (MQ=255)                                   tCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGTAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttat                                                                                                                           >  5:49272‑M2/1‑121 (MQ=255)                                   tCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttat                                                                                                                           <  6:49272‑M2/140‑20 (MQ=255)                                    acgctccaccacgtcagtagctgaaaaggagggacagctgatagaaacagaagtcactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTc                                                                                                                             >  8:134597‑M1/113‑137 (MQ=255)                                    acgcaccaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagtaggcagcatcaccTTGTGCCGCCAATAAATAACTTTTc                                                                                                                             <  7:134597‑M1/25‑1 (MQ=255)                                     aTTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttt                                                                                                                <  1:15749‑M2/149‑31 (MQ=255)                                        accaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGa                                                                                                              <  8:38269‑M1/40‑1 (MQ=255)                                             cccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcataccctaaatcagtaagttggcagcatcaccTTGTGCCGCCGATAACTATCTATTCATAAAATTagccagacacgac                                                                                                        <  4:136592‑M1/46‑7 (MQ=255)                                                              aggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTc                                                                                                                             >  6:17552‑M1/87‑111 (MQ=255)                                                              aggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTc                                                                                                                             <  5:17552‑M1/25‑1 (MQ=255)                                                                gagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTAttt                                                                                     >  6:107955‑M1/85‑149 (MQ=255)                                                                   ggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTaca                                                                                  <  5:132776‑M1/68‑1 (MQ=255)                                                                     tttGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtca                                                                                 >  8:125649‑M2/1‑87 (MQ=255)                                                                     tttGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtca                                                                                 <  7:125649‑M2/148‑62 (MQ=255)                                                                               gaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCg                                                                      <  3:24915‑M1/80‑1 (MQ=255)                                                                                    ataGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgatt                                                                 <  7:1953‑M2/149‑78 (MQ=255)                                                                                        agCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagct                                                                          <  8:105919‑M2/136‑69 (MQ=255)                                                                                        agCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagct                                                                          >  7:105919‑M2/1‑68 (MQ=255)                                                                                         ccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGt                                                              <  1:107441‑M1/88‑1 (MQ=255)                                                                                           actggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTAt                                                                                       >  3:91538‑M1/58‑120 (MQ=255)                                                                                           actggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGAGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTAt                                                                                       <  4:91538‑M1/63‑1 (MQ=255)                                                                                              aaaTTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgattttgagaac                                                         >  2:15749‑M2/1‑62 (MQ=255)                                                                                                         aaaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGa                                            >  7:38269‑M1/44‑149 (MQ=255)                                                                                                          aaacaccatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGAt                                           >  4:24915‑M1/43‑149 (MQ=255)                                                                                                              accatcatacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCAt                                       >  6:132776‑M1/39‑149 (MQ=255)                                                                                                                  cAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgattttgagaacgacagcgacttccgtcccagcc                                   <  4:132228‑M2/149‑108 (MQ=255)                                                                                                                     tacactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACAt                                <  5:13040‑M1/118‑1 (MQ=255)                                                                                                                       cactaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACAttt                              <  1:121279‑M1/120‑1 (MQ=255)                                                                                                                        actaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCga                                                                              <  7:67990‑M1/72‑1 (MQ=255)                                                                                                                        actaaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCga                                                                              >  8:67990‑M1/29‑100 (MQ=255)                                                                                                                         ctaaatcagtaagttggcagcatcaccTTGTGCCGCCGATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTa                            >  3:136592‑M1/28‑149 (MQ=255)                                                                                                                          taaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGAcgcg                                                                                                     <  7:118420‑M1/49‑1 (MQ=255)                                                                                                                          taaatcagtaagttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGAcgcg                                                                                                     >  8:118420‑M1/27‑75 (MQ=255)                                                                                                                            aGGAATTCAAAATTGTTCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgattttgagaacgacagcgacttccgtcccagccgtgccaggtg                         >  8:1953‑M2/1‑32 (MQ=255)                                                                                                                                    agttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCgca                                                                    >  8:38239‑M1/17‑98 (MQ=255)                                                                                                                                    agttggcagcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGAATTCgca                                                                    <  7:38239‑M1/82‑1 (MQ=255)                                                                                                                                           ttCTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgattttgagaacgacagcgacttccgtcccagccgtgccaggtgctgcctcagattcag          >  3:132228‑M2/1‑17 (MQ=255)                                                                                                                                           agcatcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAgg          >  2:121279‑M1/10‑149 (MQ=255)                                                                                                                                             cTTTATTTTGTGCCGggtaatgactccaacttattgatagtgttttatgttcagataatgcccgatgactttgtcatgcagctccaccgattttgagaacgacagcgacttccgtcccagccgtgccaggtgctgcctcagattcaggt        <  1:77610‑M2/149‑135 (MQ=255)                                                                                                                                               tcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGt             >  4:6527‑M1/6‑142 (MQ=255)                                                                                                                                               tcaccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGt             <  3:6527‑M1/137‑1 (MQ=255)                                                                                                                                                  ccTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTgacga   <  7:51841‑M1/147‑1 (MQ=255)                                                                                                                                                   cTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTgacgac  <  6:90633‑M1/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGAC  >  NC_000913/1292885‑1293179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.