Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,540,6470GC20.0% 57.0 / 10.3 25R648R (CGC→CGGnarZnitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (9/11);  new base C (2/3);  total (11/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGG  >  NC_000913/1540499‑1540787
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gttCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGgcg                                                                                                                                              >  2:93075/1‑149 (MQ=255)
 ttCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGgcgc                                                                                                                                             >  2:148202/1‑149 (MQ=255)
     cAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAAc                                                                                                                                         <  1:148202/149‑1 (MQ=255)
           aTACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGCGCCGAACGtatgg                                                                                                                                   <  4:67644/149‑5 (MQ=255)
                      gCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGAt                                                                                                                        <  8:62140/149‑1 (MQ=255)
                          gATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGAcc                                                                                                                    <  1:93075/149‑1 (MQ=255)
                                                         gggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCgg                                                                                         <  1:135815/145‑1 (MQ=255)
                                                         gggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCgg                                                                                         >  2:135815/1‑145 (MQ=255)
                                                          ggATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGa                                                                                      >  3:47780/1‑147 (MQ=255)
                                                                tCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACt                                                                              >  7:78033/1‑149 (MQ=255)
                                                                  cGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGc                                                                                                                  <  2:120445/111‑1 (MQ=255)
                                                                  cGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGc                                                                                                                  >  1:120445/1‑111 (MQ=255)
                                                                   ggCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCt                                                                           <  4:47780/149‑1 (MQ=255)
                                                                                    cTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCa                                                          <  8:78033/149‑1 (MQ=255)
                                                                                            ccGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGta                                                                                                              <  2:65900/89‑1 (MQ=255)
                                                                                            ccGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGta                                                                                                              >  1:65900/1‑89 (MQ=255)
                                                                                                         gCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGa                                     >  6:3109/1‑149 (MQ=255)
                                                                                                          ccccAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGAt                                    <  5:3109/149‑1 (MQ=255)
                                                                                                                                  ccagccCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCa            >  2:122317/1‑149 (MQ=255)
                                                                                                                                      cccATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTg        >  2:140526/1‑149 (MQ=255)
                                                                                                                                         caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         <  7:943/143‑1 (MQ=255)
                                                                                                                                         caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         >  8:943/3‑145 (MQ=255)
                                                                                                                                         caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         <  6:76359/143‑1 (MQ=255)
                                                                                                                                         caaCGTTCGCGCCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         >  5:76359/3‑145 (MQ=255)
                                                                                                                                          taagTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         >  4:135012/4‑144 (MQ=255)
                                                                                                                                          tACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCAttt         <  3:135012/144‑1 (MQ=255)
                                                                                                                                            cGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCgg  <  1:140526/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
GTTCGCAGGCCATACGTAAATCGCCCGATTTCAGCGCCTGGGCGGTAAATTCTGTGGGGGATAATCCGGCCTTGTCGGCTTCAGCTTTAATCCCGAGCGGGTTACGCCCCAGCTGCGGCGCAGAAGGTAGCCAGCCCATACGTTCGGCGCGAACGTTGAAATCAATCAGATGACCGCTGTACTTACTGGCATCGGCGAGCGGTGAAAGTAACTCCTGAGCAGAGACTTTTTCATAGCGCCATTGGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGG  >  NC_000913/1540499‑1540787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: