mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L001_R1_001.good.fq | 176,543 | 23,116,597 | 100.0% | 130.9 bases | 149 bases | 94.3% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L001_R2_001.good.fq | 176,543 | 22,780,399 | 100.0% | 129.0 bases | 149 bases | 97.9% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L002_R1_001.good.fq | 138,711 | 18,095,203 | 100.0% | 130.5 bases | 149 bases | 93.7% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L002_R2_001.good.fq | 138,711 | 17,821,317 | 100.0% | 128.5 bases | 149 bases | 97.2% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L003_R1_001.good.fq | 157,996 | 20,617,458 | 100.0% | 130.5 bases | 149 bases | 94.0% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L003_R2_001.good.fq | 157,996 | 20,303,429 | 100.0% | 128.5 bases | 149 bases | 97.7% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L004_R1_001.good.fq | 134,858 | 17,525,133 | 100.0% | 130.0 bases | 149 bases | 93.7% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-35_S44_L004_R2_001.good.fq | 134,858 | 17,265,329 | 100.0% | 128.0 bases | 149 bases | 97.0% |
| total | 1,216,216 | 157,524,865 | 100.0% | 129.5 bases | 149 bases | 95.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 32.3 | 3.0 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 14600 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 158 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.011 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.88841 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 23:35:09 12 Sep 2020 | 23:35:28 12 Sep 2020 | 19 seconds |
| Read alignment to reference genome | 23:35:28 12 Sep 2020 | 23:37:45 12 Sep 2020 | 2 minutes 17 seconds |
| Preprocessing alignments for candidate junction identification | 23:37:45 12 Sep 2020 | 23:38:04 12 Sep 2020 | 19 seconds |
| Preliminary analysis of coverage distribution | 23:38:04 12 Sep 2020 | 23:38:37 12 Sep 2020 | 33 seconds |
| Identifying junction candidates | 23:38:37 12 Sep 2020 | 23:39:05 12 Sep 2020 | 28 seconds |
| Re-alignment to junction candidates | 23:39:05 12 Sep 2020 | 23:39:47 12 Sep 2020 | 42 seconds |
| Resolving best read alignments | 23:39:47 12 Sep 2020 | 23:40:15 12 Sep 2020 | 28 seconds |
| Creating BAM files | 23:40:15 12 Sep 2020 | 23:40:45 12 Sep 2020 | 30 seconds |
| Tabulating error counts | 23:40:45 12 Sep 2020 | 23:40:57 12 Sep 2020 | 12 seconds |
| Re-calibrating base error rates | 23:40:57 12 Sep 2020 | 23:40:59 12 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 23:40:59 12 Sep 2020 | 23:43:34 12 Sep 2020 | 2 minutes 35 seconds |
| Polymorphism statistics | 23:43:34 12 Sep 2020 | 23:43:34 12 Sep 2020 | 0 seconds |
| Output | 23:43:34 12 Sep 2020 | 23:43:47 12 Sep 2020 | 13 seconds |
| Total | 8 minutes 38 seconds | ||
