mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L001_R1_001.good.fq | 216,890 | 28,698,024 | 100.0% | 132.3 bases | 149 bases | 96.1% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L001_R2_001.good.fq | 216,890 | 28,478,377 | 100.0% | 131.3 bases | 149 bases | 98.0% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L002_R1_001.good.fq | 171,592 | 22,604,028 | 100.0% | 131.7 bases | 149 bases | 95.8% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L002_R2_001.good.fq | 171,592 | 22,429,599 | 100.0% | 130.7 bases | 149 bases | 97.5% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L003_R1_001.good.fq | 195,706 | 25,755,224 | 100.0% | 131.6 bases | 149 bases | 95.9% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L003_R2_001.good.fq | 195,706 | 25,550,838 | 100.0% | 130.6 bases | 149 bases | 97.8% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L004_R1_001.good.fq | 167,291 | 21,964,749 | 100.0% | 131.3 bases | 149 bases | 95.6% |
| errors | Plate-2-BOP-1000-SUCB-ALE-2-flask-7_S43_L004_R2_001.good.fq | 167,291 | 21,795,820 | 100.0% | 130.3 bases | 149 bases | 97.2% |
| total | 1,502,958 | 197,276,659 | 100.0% | 131.3 bases | 149 bases | 96.8% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 40.7 | 3.3 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 16530 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 118 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.008 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.86371 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 19:40:42 10 Sep 2020 | 19:41:05 10 Sep 2020 | 23 seconds |
| Read alignment to reference genome | 19:41:06 10 Sep 2020 | 19:43:50 10 Sep 2020 | 2 minutes 44 seconds |
| Preprocessing alignments for candidate junction identification | 19:43:50 10 Sep 2020 | 19:44:13 10 Sep 2020 | 23 seconds |
| Preliminary analysis of coverage distribution | 19:44:13 10 Sep 2020 | 19:44:54 10 Sep 2020 | 41 seconds |
| Identifying junction candidates | 19:44:54 10 Sep 2020 | 19:45:33 10 Sep 2020 | 39 seconds |
| Re-alignment to junction candidates | 19:45:33 10 Sep 2020 | 19:46:24 10 Sep 2020 | 51 seconds |
| Resolving best read alignments | 19:46:24 10 Sep 2020 | 19:46:58 10 Sep 2020 | 34 seconds |
| Creating BAM files | 19:46:58 10 Sep 2020 | 19:47:36 10 Sep 2020 | 38 seconds |
| Tabulating error counts | 19:47:36 10 Sep 2020 | 19:47:51 10 Sep 2020 | 15 seconds |
| Re-calibrating base error rates | 19:47:51 10 Sep 2020 | 19:47:53 10 Sep 2020 | 2 seconds |
| Examining read alignment evidence | 19:47:53 10 Sep 2020 | 19:50:59 10 Sep 2020 | 3 minutes 6 seconds |
| Polymorphism statistics | 19:50:59 10 Sep 2020 | 19:50:59 10 Sep 2020 | 0 seconds |
| Output | 19:50:59 10 Sep 2020 | 19:51:14 10 Sep 2020 | 15 seconds |
| Total | 10 minutes 31 seconds | ||
