New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 445138388 (1.010)3 (0.040) 3/252 NT 3.2% coding (326/1503 nt) ytfR putative sugar ABC transporter ATPase
?NC_000913 = 4451398 95 (1.150)coding (341/1503 nt) ytfR putative sugar ABC transporter ATPase
Rejected: Frequency below/above cutoff threshold.

CACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGC‑‑CG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4451235‑4451383
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggc‑‑cgAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCAGATG  <  NC_000913/4451398‑4451252
                                                                                                                                                                                                                                                                                                          
CACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCtg                                                                                                                                                       >  7:230849/1‑147
CACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGC‑‑CG                                                                                                                                                     >  4:16470/1‑149
                                                                 GCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGC‑‑CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTAT                                                                                                                   >  3:298295/1‑118
                                                                 GCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGC‑‑CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTAT                                                                                                                   <  4:298295/118‑1
                                                                                                          TGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTT‑‑CGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGGGGG                                           >  6:180812/1‑149
                                                                                                                                               AGGC‑‑CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCA      <  5:212235/149‑1
                                                                                                                                                 GC‑‑CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGC        >  5:25671/1‑145
                                                                                                                                                     CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCAGATG  >  5:194657/1‑149
                                                                                                                                                     CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCAGATG  >  5:31483/1‑149
                                                                                                                                                     CGAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCAGATG  >  8:123847/1‑149
                                                                                                                                                                                                                                                                                                          
CACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCACCGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGC‑‑CG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4451235‑4451383
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggc‑‑cgAAGCGTTTGGGTTCGCGGCCTATAAATAGATTATCAGCGACCGACATATTGGGTAGCAGGTTGACTTCCTGATAGACGGTGCCAATGCCGAGTTGTTGCGCGTGGGCGGTATTTTTCGGTGAGATAGCCTGGCCTTCCAGCCAGATG  <  NC_000913/4451398‑4451252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.