Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,376,265 G→C 5.2% intergenic (+29/+14) dcuD → / ← sspB putative transporter/ClpXP protease specificity enhancing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,2650GC 5.2% 379.8 / 6.9 110intergenic (+29/+14)dcuD/sspBputative transporter/ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand):  ref base G (51/53);  new base C (3/3);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCG  >  NC_000913/3376117‑3376409
                                                                                                                                                    |                                                                                                                                                
gtggtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTg                                                                                                                                                  <  3:27887/149‑1 (MQ=255)
  ggtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGCCAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTggg                                                                                                                                                >  6:322356/1‑149 (MQ=255)
   gtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGc                                                                                                                                               <  1:143844/149‑1 (MQ=255)
      gTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCt                                                                                                                                             <  1:346898/148‑1 (MQ=255)
       ttACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGAGGACAGCGATCCCCATGGCAGGCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAACAGACAAAACAGGCCGCCTGGGCCTGt                                                                                                                                           <  5:43784/149‑1 (MQ=255)
          cGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGtttt                                                                                                                                        <  4:57471/149‑1 (MQ=255)
          cGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGtttt                                                                                                                                        <  2:64275/149‑1 (MQ=255)
          cGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGtttt                                                                                                                                        <  2:58509/149‑1 (MQ=255)
            tCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGt                                                                                                                                      <  5:233648/149‑1 (MQ=255)
             cAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTa                                                                                                                                     >  4:128100/1‑149 (MQ=255)
               ggAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                        <  8:3997/144‑1 (MQ=255)
               ggAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                        >  7:3997/1‑144 (MQ=255)
                  catGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTCttttt                                                                                                                                <  1:313995/147‑4 (MQ=255)
                  aTTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGGCAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAACACAGGCCCAGGCGGCCTGTTTTGTCttttt                                                                                                                                <  8:74305/149‑4 (MQ=255)
                     gCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTCttttt                                                                                                                                >  2:313995/1‑143 (MQ=255)
                       aGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACtt                                                                                                                               <  1:227189/145‑1 (MQ=255)
                       aGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACtt                                                                                                                               >  2:227189/1‑145 (MQ=255)
                       aGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTcac                                                                                                                            >  1:354655/1‑148 (MQ=255)
                       aGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTcac                                                                                                                            >  7:106507/1‑148 (MQ=255)
                       aGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCAAAAATCAGGCTATTTTATTAAGTCATTAAAAAGACATAACAGGACGCCTGGGCGTGTGTTGTTTTACTTgac                                                                                                                            >  2:243000/1‑148 (MQ=255)
                         gCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAAc                                                                                                                         <  4:226499/149‑1 (MQ=255)
                           gTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAAcgc                                                                                                                       >  2:285139/1‑149 (MQ=255)
                            tttCGCCTTTTGAGGAGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAAcgcg                                                                                                                      >  4:343805/1‑149 (MQ=255)
                                gCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTaa                                                                                                                   <  1:275801/148‑1 (MQ=255)
                                   ttttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGcc                                                                                                               >  3:355953/1‑149 (MQ=255)
                                   ttttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGcc                                                                                                               >  7:252555/1‑149 (MQ=255)
                                       gCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGt                                                                                                                     <  4:320905/139‑1 (MQ=255)
                                        cGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCttttta                                                                                                                               <  4:242938/128‑5 (MQ=38)
                                        cGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCttttta                                                                                                                               >  3:242938/1‑124 (MQ=38)
                                        cGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGGGAATATGATTGCCACAATCACGCGATTTTAGTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTGTGTATTACTTCACAAGGCGTAATGCCGGTCg                                                                                                          >  4:90881/1‑149 (MQ=255)
                                             gtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGaccac                                                                                                     >  2:386692/1‑149 (MQ=255)
                                              tgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCGGGGCCTGTGTTGTATTACTTCACAACGCGTAATGCCGGTCGaccacc                                                                                                    >  5:299741/1‑149 (MQ=255)
                                                  gCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgt                                                                                                <  4:120513/149‑1 (MQ=255)
                                                  gCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgt                                                                                                >  3:217311/1‑149 (MQ=255)
                                                   ctgACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgtg                                                                                               <  1:304757/147‑1 (MQ=255)
                                                     gACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgtggt                                                                                             <  3:343805/149‑1 (MQ=255)
                                                     gACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCgtggt                                                                                             <  5:316053/149‑1 (MQ=255)
                                                       caccGAGCCACATGGCAGTCGGGTTCGTGGTGAATATGATAGCCACAATCACGCTTTTTTATTAAGTCATTAAAAAGACAAACCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAAGGCCGGTCGACCACCGCGtggtgg                                                                                           <  3:204527/146‑1 (MQ=255)
                                                           gATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGc                                                                                       <  1:285139/149‑1 (MQ=255)
                                                            aTCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCg                                                                                      >  6:382177/1‑149 (MQ=255)
                                                             tCCCCATGGCATTCGGTATCGTGCTCAATATGAATGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGATTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCgg                                                                                     <  1:243000/149‑1 (MQ=255)
                                                             tCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCgg                                                                                     <  5:136213/149‑1 (MQ=255)
                                                               cccATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAg                                                                                   >  1:160747/1‑149 (MQ=255)
                                                                 cATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCATCGCGTGGTGGCTGCGGAGGt                                                                                 <  7:399825/149‑1 (MQ=255)
                                                                   tGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTc                                                                               <  2:354655/149‑1 (MQ=255)
                                                                        gTCTGTTTCGTTGTGAATATGATTGCAACAATCACGCTATTTTATTAAGTCATTAAAAATACAAAACAGGCCGCCTGGGCCTGTTTTGTATTAATTAACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGt                                                                          <  2:308381/149‑1 (MQ=255)
                                                                                 gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGGCAGGATGa                                                                  <  1:386692/148‑1 (MQ=255)
                                                                                  tggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCTCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAgt                                                                <  2:13217/149‑1 (MQ=255)
                                                                                    ttgAATATCATTGCAACAATCACGCTATTTTATTAAGTCATTAAAAATACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGCCCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAgtg                                                               <  5:79024/147‑1 (MQ=255)
                                                                                    gtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCa                                                                        >  7:80837/1‑139 (MQ=255)
                                                                                           tGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAACCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCatcatc                                                       <  2:211304/149‑1 (MQ=255)
                                                                                            gATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCg                                                      >  5:3113/1‑149 (MQ=255)
                                                                                              ttGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTg                                                    >  4:316368/1‑149 (MQ=255)
                                                                                                  cacaATCACGCTATTTTATTAAGTCATTAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTg                                               >  7:170264/1‑149 (MQ=255)
                                                                                                   ccaATCACGCTATTTTATTAAGTCATTATAAAGACTAAACAGGGCGCCTGGGCCTGTTTTGTATTAATTGAAATCGCGTAATGCCGGTCGACCACCGCGTTGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTTATCTg                                               <  4:218409/148‑1 (MQ=255)
                                                                                                     aaTCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGc                                             >  2:5194/1‑149 (MQ=255)
                                                                                                     aaTCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGc                                             >  8:221746/1‑149 (MQ=255)
                                                                                                     aaTCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGc                                             <  8:106507/149‑1 (MQ=255)
                                                                                                     aaTCACGCTATTTTATTAAGTCATTAAAAAGACAAAACACGCCGCCTCGGCCTGTTTTGTATTACTTAAAAAAGCGTAATGACTGACGACCACCGCGTGGTGGCTGCTGAGGTTCATCGTCAGGATCAGTGTCATCAACGTGATCTgga                                             >  5:358757/1‑148 (MQ=255)
                                                                                                       tCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCtt                                           <  6:369464/149‑1 (MQ=255)
                                                                                                       tCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCtt                                           <  1:114274/149‑1 (MQ=255)
                                                                                                         aCGCTATTTTATTAAGTCATTAAATAGACTAAACTGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTATTGCCGGTCGACCACCGCGTGGTGGATGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTg                                          <  6:358757/148‑1 (MQ=255)
                                                                                                         aCGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCatcatc                                                       <  4:297200/135‑1 (MQ=255)
                                                                                                         aCGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCatcatc                                                       >  3:297200/1‑135 (MQ=255)
                                                                                                         aCGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGt                                         <  5:51192/149‑1 (MQ=255)
                                                                                                         aCGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGt                                         >  6:202347/1‑149 (MQ=255)
                                                                                                           gCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCg                                       >  2:230590/1‑149 (MQ=255)
                                                                                                           gCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCg                                       >  2:189194/1‑149 (MQ=255)
                                                                                                               ttttATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGcc                                     <  4:217311/147‑1 (MQ=255)
                                                                                                               ttttATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCAt                                   <  6:211047/149‑1 (MQ=255)
                                                                                                                 ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATc                                  <  5:382177/148‑1 (MQ=255)
                                                                                                                  tattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCaa                                >  6:301458/1‑149 (MQ=255)
                                                                                                                       aGTCATTAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                           <  8:170264/148‑1 (MQ=255)
                                                                                                                               aaaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAAc                   >  2:269447/1‑149 (MQ=255)
                                                                                                                                aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGGTTGTCGGCATCAATAACCGACATAACg                  >  7:107721/1‑149 (MQ=255)
                                                                                                                                aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACg                  >  3:221612/1‑149 (MQ=255)
                                                                                                                                 aaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACgg                 <  5:301458/149‑1 (MQ=255)
                                                                                                                                     cAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTc             <  5:322356/149‑1 (MQ=255)
                                                                                                                                      aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCg                                                                                      >  8:303183/1‑75 (MQ=255)
                                                                                                                                      aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCg                                                                                      <  7:303183/75‑1 (MQ=255)
                                                                                                                                      aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCg            >  8:4049/1‑149 (MQ=255)
                                                                                                                                      aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCg            >  4:61384/1‑149 (MQ=255)
                                                                                                                                      aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCg            >  3:175842/1‑149 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGa                                                                     <  5:309669/90‑1 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGa                                                                     >  6:309669/1‑90 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                           >  7:378302/1‑132 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                           >  7:383648/1‑132 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                           <  8:378302/132‑1 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                           <  8:383648/132‑1 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGtt          <  1:337442/149‑1 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGtt          <  8:144887/149‑1 (MQ=255)
                                                                                                                                        aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGtt          >  4:52780/1‑149 (MQ=255)
                                                                                                                                           aGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCa                                    <  2:326654/120‑1 (MQ=255)
                                                                                                                                           aGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCa                                    >  1:326654/1‑120 (MQ=255)
                                                                                                                                            ggCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGGGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCt      <  3:316368/149‑1 (MQ=255)
                                                                                                                                             gccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTg     >  2:106283/1‑149 (MQ=255)
                                                                                                                                             gccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTg     >  1:71496/1‑149 (MQ=255)
                                                                                                                                              ccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGc    <  2:160747/149‑1 (MQ=255)
                                                                                                                                              ccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGc    >  1:39276/1‑149 (MQ=255)
                                                                                                                                                gccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCg  <  8:327381/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GTGGTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCG  >  NC_000913/3376117‑3376409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: