Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,906,828 A→T 5.0% intergenic (‑261/+25) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,906,8280AT 5.0% 338.6 / 5.7 111intergenic (‑261/+25)bglG/phoUtranscriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base A (52/53);  new base T (3/3);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

Only 100 of 114 total aligned reads displayed.

ACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATA  >  NC_000913/3906682‑3906976
                                                                                                                                                  |                                                                                                                                                    
aCGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTaa                                                                                                                                                     <  8:291525/148‑1 (MQ=255)
aCGAACATCCAGGTTAGGAAATTAATTTAATTGCGTGCAGAGAATATAGCTATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTaa                                                                                                                                                     <  5:359779/148‑1 (MQ=255)
 cGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAACATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                   <  1:107113/149‑1 (MQ=255)
  gAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAttt                                                                                                                                                  <  3:37634/149‑1 (MQ=255)
    aCATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAt                                                                                                                                                >  4:63549/1‑149 (MQ=255)
      aaccAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTg                                                                                                                                              >  2:127904/3‑149 (MQ=255)
      aTCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTg                                                                                                                                              <  8:338006/149‑1 (MQ=255)
       tCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTgg                                                                                                                                             <  4:234692/149‑1 (MQ=255)
        ccAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTgg                                                                                                                                             <  1:276469/148‑1 (MQ=255)
        ccAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTgg                                                                                                                                             >  2:276469/1‑148 (MQ=255)
          aGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTATTTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAt                                                                                                                                          >  7:282026/1‑149 (MQ=255)
            gTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAttt                                                                                                                                        <  7:237124/149‑1 (MQ=255)
                ggAAATTAATTTAATTGCGTGCAGAGAATATAGCAATGGGCTTTTGATATAAATATGACCATGCTAGGAGTTTTTAACTTTGTGTAATATTTGTAATTTATAAAGTTAAATATAACAAATCCCAATAATTAATTTATTGGGATTTGtat                                                                                                                                    <  2:354464/149‑3 (MQ=255)
                 gAAATTAATTTTATTGAGTGCATAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCATTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATATCAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                   <  8:631/149‑1 (MQ=255)
                 gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTAATATATAACAAATCCCAATAACTTAATTATTGGGATTTGttat                                                                                                                                   <  1:359249/149‑4 (MQ=39)
                 gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTATGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTg                                                                                                                                   <  4:16293/149‑1 (MQ=255)
                    attaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGGGATTTGTATGGt                                                                                                                                 >  5:304852/1‑148 (MQ=255)
                       aatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGaa                                                                                                                              <  5:250677/148‑1 (MQ=255)
                         tttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatta                                                                                                                           >  8:112519/1‑149 (MQ=255)
                         tttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatta                                                                                                                           >  3:305108/1‑149 (MQ=255)
                          ttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattat                                                                                                                          >  5:42833/1‑149 (MQ=255)
                            aattGGCTGCAGATAAAATATCAATGCGCTATTTATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATCACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                         <  6:302394/148‑1 (MQ=255)
                            aattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                         <  1:127904/148‑1 (MQ=255)
                                   tacAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAtt                                                                                                                                               >  7:186591/3‑119 (MQ=255)
                                   tacAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAtt                                                                                                                                               <  8:186591/117‑1 (MQ=255)
                                   tGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTa                                                                                                                 <  1:277683/149‑1 (MQ=255)
                                      agagAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCt                                                                                                              >  5:250384/1‑149 (MQ=255)
                                       gagaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAttt                                                                                                                                        >  7:253899/1‑122 (MQ=255)
                                       gagaAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAttt                                                                                                                                        <  8:253899/122‑1 (MQ=255)
                                           aaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                         <  2:382344/149‑1 (MQ=255)
                                            aaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTcccc                                                                                                        <  4:330152/149‑1 (MQ=255)
                                              tAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCTGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGc                                                                                                      <  6:64049/149‑1 (MQ=255)
                                                gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTGTTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCca                                                                                                    <  6:55122/149‑1 (MQ=255)
                                                gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCca                                                                                                    <  7:188327/149‑1 (MQ=255)
                                                 cAATGCGCTATTGATAAAAATATGAACATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGTGATTTGTCTTGTGAATTATTTTTAGCTATCTTTCCACGCcag                                                                                                   >  1:304632/1‑149 (MQ=255)
                                                   aTGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGAtt                                                                                                                                         >  1:335243/1‑109 (MQ=255)
                                                   aTGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGAtt                                                                                                                                         <  2:335243/109‑1 (MQ=255)
                                                        cTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                            <  3:63549/149‑1 (MQ=255)
                                                         tATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                            >  5:104642/1‑148 (MQ=255)
                                                           ttGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                            <  6:104642/146‑1 (MQ=255)
                                                             gATAAAAATATGACCTTGCTCGCAGTTATTATCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCa                                                                                        <  8:282026/148‑1 (MQ=255)
                                                             gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCTCCAGCAGTTTATCAAg                                                                                       >  5:327255/1‑149 (MQ=255)
                                                              aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                      >  7:291938/1‑149 (MQ=255)
                                                              aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                      >  6:297836/1‑149 (MQ=255)
                                                                aaaaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCt                                                                                     >  7:41377/1‑148 (MQ=255)
                                                                  aaaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCAt                                                                                  >  6:336675/1‑149 (MQ=255)
                                                                    atatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCg                                                                                <  2:310419/149‑1 (MQ=255)
                                                                         aCCATGCTCGCAGTTATTAACTTTGTTTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAAGTAAGTTATTGGGATTTCTCTTGTGAATTATTTCTCTCTATCTTTCCCAGCCAGCAGTTTATCAAGCTCATCGCCAcc                                                                           >  4:297309/1‑149 (MQ=255)
                                                                          ccATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCg                                                                          <  1:22820/149‑1 (MQ=255)
                                                                           cATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGa                                                                         >  2:221171/1‑149 (MQ=255)
                                                                             tgcttgctGTTTTTTTCTTTGTGTTTTTTTTGGATTTTTTAATGTTATATATATCATTTGCGATTATTTTTGTTTTTGGGATTTGTGTGGTGATTTATTTGTCGCTTTCTTTCCCCGCGAGCAGTTTATCCAGCTCATCGCCACCGAc                                                                        <  2:351299/148‑1 (MQ=255)
                                                                                tCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCtct                                                                                                            <  8:113057/109‑3 (MQ=255)
                                                                                tCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCtct                                                                                                            >  7:113057/1‑107 (MQ=255)
                                                                                   cAGTTATTAACTTTGTGTTATTTTAGGAATTTATAAAGTTTTATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACgg                                                                 <  1:262370/149‑1 (MQ=255)
                                                                                         ttaACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATcc                                                           <  7:112519/149‑1 (MQ=255)
                                                                                            aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        <  8:198973/149‑1 (MQ=255)
                                                                                            aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        <  6:361371/149‑1 (MQ=255)
                                                                                            aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        >  1:205755/1‑149 (MQ=255)
                                                                                             cTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGc                                                                                                      >  3:254364/1‑102 (MQ=255)
                                                                                                  tgtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTc                                                  <  5:297836/149‑1 (MQ=255)
                                                                                                  tgtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTATGGTGAATTATTTGTCGATATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCtt                                                   >  5:99955/1‑148 (MQ=255)
                                                                                                    tAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                         <  8:210319/92‑1 (MQ=255)
                                                                                                     aaTTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACg                                               >  8:179037/1‑149 (MQ=255)
                                                                                                      aTTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACg                                               >  3:94526/1‑148 (MQ=255)
                                                                                                       ttttAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTTTTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACg                                               >  3:139135/1‑147 (MQ=255)
                                                                                                           aGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtag                                            >  1:132813/1‑146 (MQ=255)
                                                                                                                 ttATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGAt                                    >  6:210902/1‑148 (MQ=255)
                                                                                                                  tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATa                                   >  5:199211/1‑148 (MQ=255)
                                                                                                                     aaaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACt                               >  6:178629/1‑149 (MQ=255)
                                                                                                                     aaaGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACt                               >  3:104298/1‑149 (MQ=255)
                                                                                                                      aacttATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTc                              <  2:51271/146‑1 (MQ=255)
                                                                                                                       aGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        >  4:370837/1‑122 (MQ=255)
                                                                                                                       aGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        <  3:370837/122‑1 (MQ=255)
                                                                                                                        gTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTcac                            <  4:94526/149‑1 (MQ=255)
                                                                                                                             atataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAata                       >  4:103810/1‑149 (MQ=255)
                                                                                                                             atataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAat                        <  4:104298/148‑1 (MQ=255)
                                                                                                                               cagaCAAATCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                     >  3:22727/4‑149 (MQ=255)
                                                                                                                               ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                     >  5:42775/1‑149 (MQ=255)
                                                                                                                                taACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcccc                                                     >  8:151548/1‑116 (MQ=255)
                                                                                                                                 aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACaa                          <  6:284520/142‑1 (MQ=255)
                                                                                                                                 aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACaa                          >  5:284520/1‑142 (MQ=255)
                                                                                                                                 aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                     >  4:354546/1‑147 (MQ=255)
                                                                                                                                 aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATAtt                     <  3:354546/147‑1 (MQ=255)
                                                                                                                                  aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCt                   <  6:250384/148‑1 (MQ=255)
                                                                                                                                  aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  <  1:35882/149‑1 (MQ=255)
                                                                                                                                  aCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  >  3:213599/1‑149 (MQ=255)
                                                                                                                                  aCAAATCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  >  2:269754/1‑149 (MQ=255)
                                                                                                                                   cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTaga                                        <  7:239134/126‑1 (MQ=255)
                                                                                                                                   cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTaga                                        >  8:239134/1‑126 (MQ=255)
                                                                                                                                   cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACATATATTCTgg                 <  1:296011/149‑1 (MQ=255)
                                                                                                                                   cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg                 <  7:179037/149‑1 (MQ=255)
                                                                                                                                    aaaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTgg                 >  7:232886/1‑148 (MQ=255)
                                                                                                                                    aaaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACCTAGTAGAAGATAACATAACAAATATTCTgg                 >  5:96352/1‑148 (MQ=255)
                                                                                                                                      aTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg              >  2:69730/1‑149 (MQ=255)
                                                                                                                                      aTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCAAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg              >  4:249554/1‑149 (MQ=255)
                                                                                                                                         ccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtag                                            <  4:220972/116‑1 (MQ=255)
                                                                                                                                         ccAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCgg           >  2:118339/1‑149 (MQ=255)
                                                                                                                                           aataatTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGAGACGGAAATCCTGCACCTTCACGTAGTAGAAGATATACTCACAAATATTCTGGCACCGGTc         >  3:211265/1‑149 (MQ=255)
                                                                                                                                             taatTAAGTTATTGGGATTTGACTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGc       <  8:392701/149‑1 (MQ=255)
                                                                                                                                                  aaGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATa  >  6:117967/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATA  >  NC_000913/3906682‑3906976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: