Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,128,041 C→T 5.9% intergenic (‑147/+37) yiiX ← / ← metJ putative lipid binding hydrolase, DUF830 family protein/transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,0410CT 5.9% 212.8 / 2.5 67intergenic (‑147/+37)yiiX/metJputative lipid binding hydrolase, DUF830 family protein/transcriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base C (30/33);  new base T (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

TTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAA  >  NC_000913/4127897‑4128186
                                                                                                                                                |                                                                                                                                                 
ttAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCat                                                                                                                                                 <  7:44635‑M2/147‑3 (MQ=255)
    aCGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCatcaggc                                                                                                                                            <  8:109843‑M2/148‑8 (MQ=255)
         cATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTc                                                                                                                                       >  6:251208/1‑148 (MQ=255)
             tataAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTc                                                                                                                                  >  4:322011/1‑149 (MQ=255)
                aaaaaGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCcgc                                                                                                                               >  4:67462/1‑149 (MQ=255)
                   aaGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATGCGCTACGCTTatcaggcctacgtcatattgcaa                                                                                                                            <  3:305174‑M2/149‑24 (MQ=255)
                        gTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGctct                                                                                                                       <  1:47586/149‑1 (MQ=255)
                        gTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGctct                                                                                                                       <  1:105183/149‑1 (MQ=255)
                             tCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCtttttt                                                                                                                  >  6:28409/1‑149 (MQ=255)
                               gCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATGCGCTACGCTTatcaggcctacgtcatattgcaatttattgaattt                                                                                                                <  3:229853‑M2/149‑36 (MQ=255)
                               gCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTg                                                                                                                 <  4:178361/148‑1 (MQ=255)
                                cAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCt                                                                                                               <  2:84501/149‑1 (MQ=255)
                                cAGACAACGTGCTCGTTGTTTATGCCGGATCCGGCGTGAACGCCATATCCGCCCGACAAGTTCGTGCAAATTCAATAAATAGCAAAATGACGTACGCCTGATAAGAGTAGCGCATCAGGCGATTc                                                                                                                                       >  5:155128/1‑125 (MQ=255)
                                 aGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCtt                                                                                                              <  4:116883/149‑1 (MQ=255)
                                  gACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCtttt                                                                                                                    >  7:43629/1‑142 (MQ=255)
                                  gACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCtttt                                                                                                                    <  8:43629/142‑1 (MQ=255)
                                   aCAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATGCGCTACGCTTatcaggcctacgtcatattgcaatttattgaatttgcac                                                                                                            >  4:305174‑M2/1‑110 (MQ=255)
                                   aCAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTa                                                                                                            <  5:385017/149‑1 (MQ=255)
                                   aCAACGTGCTCGTTGTTTATGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGACGCTCTTTTTTGCTTTa                                                                                                            <  4:35955/149‑1 (MQ=255)
                                           cTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTccc                                                                                                    >  5:164317/1‑149 (MQ=255)
                                              gttgttTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCAc                                                                                                  <  5:18085/148‑1 (MQ=255)
                                              gttgttTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACg                                                                                                 >  5:307264/1‑149 (MQ=255)
                                                     aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCg                                                                                           <  3:286016/148‑1 (MQ=255)
                                                      tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCggg                                                                                         <  5:251208/149‑1 (MQ=255)
                                                       gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCCTCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGt                                                                                        >  5:114780/1‑149 (MQ=255)
                                                        ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATGCGCTACGCTTatcaggcctacgtcatattgcaatttattgaatttgcacgaacttgtaggccggataag                                                                                        >  4:229853‑M2/1‑89 (MQ=255)
                                                         cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTcc                                                                                                     >  1:134002/1‑134 (MQ=255)
                                                         cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTcc                                                                                                     <  2:134002/134‑1 (MQ=255)
                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTaa                                                                                     <  8:44635/149‑1 (MQ=255)
                                                            aTGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATc                                                                                   <  4:200750/149‑1 (MQ=255)
                                                                ggcgTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCa                                                                               <  5:28409/149‑1 (MQ=255)
                                                                   gTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTa                                                                                                            <  1:291614/117‑1 (MQ=255)
                                                                   gTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTa                                                                                                            >  2:291614/1‑117 (MQ=255)
                                                                    tGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCAtctc                                                                           <  5:292861/149‑1 (MQ=255)
                                                                      aaCGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCAc                                                                         <  6:307264/149‑1 (MQ=255)
                                                                           cTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCACATCTCACGCAt                                                                     >  4:156435/1‑148 (MQ=255)
                                                                              aTCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCCCACGCATGAt                                                                  <  5:135857/148‑1 (MQ=255)
                                                                               tCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGAtct                                                                >  8:90303/1‑149 (MQ=255)
                                                                                 cGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATctct                                                              <  5:135637/149‑1 (MQ=255)
                                                                                    ccTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCtttt                                                           <  7:109843/149‑1 (MQ=255)
                                                                                      tACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGc                                                         >  7:383293/1‑149 (MQ=255)
                                                                                           gTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCtt                                                                                                              >  8:224579/1‑91 (MQ=255)
                                                                                               gTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCgg                                                >  2:57196/1‑149 (MQ=255)
                                                                                               gTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCg                                                 <  3:322011/148‑1 (MQ=255)
                                                                                                tGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCgg                                                >  7:405310/1‑148 (MQ=255)
                                                                                                     aTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtc                                          <  3:124488/149‑1 (MQ=255)
                                                                                                     aTTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtc                                          >  6:93484/1‑149 (MQ=255)
                                                                                                      ttCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTtcg                                         >  6:71385/1‑149 (MQ=255)
                                                                                                         aaTAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCCCGCATGCTCTCTTTTGCCGCTTCCGGGATTtcgtcg                                      >  7:337129/1‑149 (MQ=255)
                                                                                                           tAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCt                                    <  5:350221/149‑1 (MQ=255)
                                                                                                             aaTTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTg                                   <  7:163905/148‑1 (MQ=255)
                                                                                                                    atatGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCttt                           <  7:154573/149‑1 (MQ=255)
                                                                                                                         aCGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGc                       <  4:291221/148‑1 (MQ=255)
                                                                                                                         aCGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGGTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTACGGGATTTCGTCGCTGCGCTCTTTACg                        >  5:304766/1‑147 (MQ=255)
                                                                                                                             aGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCCCTATTTTTTGCTTTAGTAtt                                                                                                       >  2:131949/1‑64 (MQ=255)
                                                                                                                              ggCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCg                 <  8:405310/149‑1 (MQ=255)
                                                                                                                              ggCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCACCATCTCTCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATc                  <  5:311255/148‑1 (MQ=255)
                                                                                                                               gCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCgg                >  6:348944/1‑149 (MQ=255)
                                                                                                                                   gATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCtt                                                             <  7:43379/100‑1 (MQ=255)
                                                                                                                                   gATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCtt                                                             >  8:43379/1‑100 (MQ=255)
                                                                                                                                    aTAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGcatca           >  4:162865/1‑149 (MQ=255)
                                                                                                                                    aTAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGcatc            >  8:133198/1‑148 (MQ=255)
                                                                                                                                      aaGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCatcatc         >  3:330143/1‑149 (MQ=255)
                                                                                                                                       aGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCatcatc         <  6:164317/148‑1 (MQ=255)
                                                                                                                                         cGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCTTCGCTGCGCTCTTTACGCAGATCGGCACCATCCgg      >  6:214062/1‑149 (MQ=255)
                                                                                                                                          gTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCgg      >  7:63269/1‑148 (MQ=255)
                                                                                                                                           tAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGc     >  5:284100/1‑148 (MQ=255)
                                                                                                                                             gcgcATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCaaa  <  4:226169/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TTAAACGTCCATGTATAAAAAGCGGTGGGTCGCAGACAACGTGCTCGTTGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGACGTAGGCCTGATAAGCGTAGCGCATCAGGCGATTCCACTCCGCGCCGCTCTTTTTTGCTTTAGTATTCCCACGTCTCCGGGTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAA  >  NC_000913/4127897‑4128186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: