Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,204,203 G→A 5.2% R295H (CGC→CAC)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,2030GA 5.2% 249.6 / 2.2 77R295H (CGC→CAC) zraRfused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base A (2/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

TCGCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAG  >  NC_000913/4204055‑4204351
                                                                                                                                                    |                                                                                                                                                    
tcGCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCg                                                                                                                                                      >  6:158307/1‑149 (MQ=255)
  gCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTGGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGcc                                                                                                                                                    <  2:71464/149‑1 (MQ=255)
   ccGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCa                                                                                                                                                   <  8:306880/149‑1 (MQ=255)
               gTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTctacta                                                                                                                                       <  2:379175/149‑1 (MQ=255)
                 gCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATc                                                                                                                                     >  2:255908/1‑149 (MQ=255)
                    tCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGcc                                                                                                                                  <  8:97251/149‑1 (MQ=255)
                    tCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGcc                                                                                                                                  <  6:6339/149‑1 (MQ=255)
                      tgctgcGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCt                                                                                                                                 >  4:360563/1‑148 (MQ=255)
                         tgcgtgcgATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAAt                                                                                                                             >  1:25667/1‑149 (MQ=255)
                         tgcgtgcgATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAAt                                                                                                                             >  7:88873/1‑149 (MQ=255)
                               cgATTCAGGAGCGCGAAGTTCCGCGTGTCGGTACCAACCAGATTATCTCGGGTGATGTCCGGCTGATTTCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGACTGAATgtggtg                                                                                                                       <  4:209196/149‑1 (MQ=255)
                                 aTTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGc                                                                                                                     >  2:325153/1‑149 (MQ=255)
                                  ttCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGc                                                                                                                     >  7:229269/1‑148 (MQ=255)
                                     aGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGAtt                                                                                                                 <  4:133495/149‑1 (MQ=255)
                                      ggAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGAGTATCTCGGTTGCTGGCCGGCTGATTGCGGCGACCCATCGCGATCTTGCGGCAGCGGTGAATGCCGGGCGTGTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGAtg                                                                                                                 >  4:55493/1‑147 (MQ=255)
                                        aGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTTATGTCCGGCTGATTTAGGATACCCATCGCGATCTTGCCTCAGATGTGAATGCCGGGCGTTTTCTCCAGGATCTCGACTATCGCCTTACTGTGGTGGCGATTGaa                                                                                                              <  6:258842/149‑1 (MQ=255)
                                        aGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGaa                                                                                                              <  3:357989/149‑1 (MQ=255)
                                           gcgAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTa                                                                                                           >  8:346095/1‑149 (MQ=255)
                                           gcgAAGTTCAGCGTGTCGGTAGCAACCAGATAATGTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCATGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGt                                                                                                            >  8:133121/1‑148 (MQ=255)
                                            ctaaGTTCAGCGTGTCGGTAGCAACCAGTTTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCTCGTTCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCATGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTAc                                                                                                          <  5:14186/147‑1 (MQ=255)
                                            cgAAGGTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTAc                                                                                                          <  4:332148/149‑1 (MQ=255)
                                             gAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCt                                                                                                                                 >  1:312067/1‑125 (MQ=255)
                                             gAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCt                                                                                                                                 <  2:312067/125‑1 (MQ=255)
                                             gAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCACCTGAATCTCGTGGCGATTGAAGTAc                                                                                                          >  1:218020/1‑148 (MQ=255)
                                                gTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATc                                                                                                      <  6:17329/149‑1 (MQ=255)
                                                      cGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCg                                                                                                <  1:255908/149‑1 (MQ=255)
                                                          tcagTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCaa                                                                                            >  2:8000/4‑149 (MQ=255)
                                                            ggTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTa                                                                                                           >  3:213254/1‑132 (MQ=255)
                                                            ggTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTa                                                                                                           <  4:213254/132‑1 (MQ=255)
                                                            ggTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAAcg                                                                                          >  3:167872/1‑149 (MQ=255)
                                                                gCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAAcggcgg                                                                                      <  6:315083/149‑1 (MQ=255)
                                                                gCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAAcggcg                                                                                       <  2:237846/148‑1 (MQ=255)
                                                                 cAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     <  2:25667/149‑1 (MQ=255)
                                                                  aaCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  5:227564/1‑148 (MQ=255)
                                                                  aaCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  5:232767/1‑148 (MQ=255)
                                                                  aaCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  6:217582/1‑148 (MQ=255)
                                                                  aaCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  1:233047/1‑148 (MQ=255)
                                                                  aaCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  1:238211/1‑148 (MQ=255)
                                                                   aCCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  3:54731/1‑147 (MQ=255)
                                                                    ccAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAg                                                                                  <  1:192401/149‑1 (MQ=255)
                                                                     cAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCggg                                                                                     >  7:318768/1‑145 (MQ=255)
                                                                      aGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGTAGat                                                                                <  6:232767/149‑1 (MQ=255)
                                                                      aGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGTAGat                                                                                <  6:227564/149‑1 (MQ=255)
                                                                       gATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCACCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGata                                                                               >  4:272670/1‑149 (MQ=255)
                                                                           aTCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCACCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATcc                                                                            <  3:272670/148‑1 (MQ=255)
                                                                            tgtcGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCct                                                                          <  6:60958/147‑1 (MQ=255)
                                                                                ggTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCct                                                                          <  8:229269/145‑1 (MQ=255)
                                                                                ggTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTctgc                                                                      >  1:210697/1‑149 (MQ=255)
                                                                                        tCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGAGCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGctggctgg                                                               <  1:268744/148‑1 (MQ=255)
                                                                                          cGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCa                                                            >  1:299025/1‑149 (MQ=255)
                                                                                          cGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCACCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATc                                                                                                      >  4:212817/1‑107 (MQ=255)
                                                                                          cGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCACCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATc                                                                                                      <  3:212817/107‑1 (MQ=255)
                                                                                              tGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCAtttt                                                        >  1:109537/1‑149 (MQ=255)
                                                                                                    cggcgACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAg                                                  <  5:374042/149‑1 (MQ=255)
                                                                                                    cggcgACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAg                                                  <  6:174527/149‑1 (MQ=255)
                                                                                                           ccATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTg                                           >  4:143172/1‑149 (MQ=255)
                                                                                                           ccATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTg                                           >  3:331014/1‑149 (MQ=255)
                                                                                                           ccATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTg                                           >  6:18137/1‑149 (MQ=255)
                                                                                                           ccATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTg                                           >  1:185008/1‑149 (MQ=255)
                                                                                                            cATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGc                                          <  3:53378/149‑1 (MQ=255)
                                                                                                             aTCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGcc                                         >  3:2556/1‑149 (MQ=255)
                                                                                                             aTCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGcc                                         <  5:18137/149‑1 (MQ=255)
                                                                                                              tCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGcc                                         >  7:189058/1‑148 (MQ=255)
                                                                                                                    tCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGc                                     <  4:249922/146‑1 (MQ=255)
                                                                                                                    tCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGc                                     >  3:249922/1‑146 (MQ=255)
                                                                                                                        gCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTaa                                 <  1:8000/146‑1 (MQ=255)
                                                                                                                        gCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTa                                  <  4:54731/145‑1 (MQ=255)
                                                                                                                          cGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGc                             >  2:150991/1‑148 (MQ=255)
                                                                                                                            cAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAg                          <  8:88873/149‑1 (MQ=255)
                                                                                                                                     aTGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTaaaa                 <  1:325153/149‑1 (MQ=255)
                                                                                                                                      tGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAg                <  5:158307/149‑1 (MQ=255)
                                                                                                                                      tGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAg                <  7:346095/149‑1 (MQ=255)
                                                                                                                                      tGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAg                >  7:166386/1‑149 (MQ=255)
                                                                                                                                      tGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAg                <  4:83691/149‑1 (MQ=255)
                                                                                                                                         cGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGtt             <  2:185008/149‑1 (MQ=255)
                                                                                                                                           ggCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGtttt           <  3:99492/149‑1 (MQ=255)
                                                                                                                                              gTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTctg                                                                       >  6:84521/1‑86 (MQ=255)
                                                                                                                                              gTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTctg                                                                       <  5:84521/86‑1 (MQ=255)
                                                                                                                                                    gCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAg  >  1:114783/1‑149 (MQ=255)
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TCGCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAGCAACCAGATTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTTTTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCTCTGCTGGCTGGCCATTTTCTGCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGTTTTACGCCCCAG  >  NC_000913/4204055‑4204351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: