Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,272,156 G→A 5.6% V572V (GTC→GTT)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,272,1560GA 5.6% 224.3 / 2.4 72V572V (GTC→GTT) uvrAATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base A (2/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

GCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGT  >  NC_000913/4272012‑4272301
                                                                                                                                                |                                                                                                                                                 
gCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCa                                                                                                                                               >  4:146588/1‑149 (MQ=255)
  cgcgTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACt                                                                                                                                             >  3:290811/1‑149 (MQ=255)
      tGCGGCTGTCAGCTTCAGCACTTTTTCCGGATTCGGCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGGCGCTCATGTACTGCCGGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAc                                                                                                                                         <  5:343554/149‑1 (MQ=255)
        cgcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCAcc                                                                                                                                        <  1:307359/148‑1 (MQ=255)
         gcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc                                                                                                                                                 >  4:331959/1‑138 (MQ=255)
         gcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGAc                                                                                                                                                 <  3:331959/138‑1 (MQ=255)
                   ttCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACAcc                                                                                                                            >  2:98123/1‑149 (MQ=255)
                        cACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGa                                                                                                                                                  >  8:144919/1‑122 (MQ=255)
                        cACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGa                                                                                                                                                  <  7:144919/122‑1 (MQ=255)
                         aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGcc                                                                                                                      >  8:84891/1‑149 (MQ=255)
                                     ttCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTc                                                                                                          >  6:26936/1‑149 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGa                                                                                                        <  3:146588/148‑1 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGAt                                                                                                       >  1:27437/1‑149 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCAACCACTTCACCGCCGTGAacac                                                                                                                             >  1:229948/1‑127 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCAACCACTTCACCGCCGTGAacac                                                                                                                             >  1:235656/1‑127 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCAACCACTTCACCGCCGTGAacac                                                                                                                             <  2:235656/127‑1 (MQ=255)
                                        gCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCAACCACTTCACCGCCGTGAacac                                                                                                                             <  2:229948/127‑1 (MQ=255)
                                          cGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATca                                                                                                     >  3:367704/1‑149 (MQ=255)
                                                gcgTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGt                                                                                               <  2:149402/149‑1 (MQ=255)
                                                    ttcttcGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgc                                                                                           >  1:398771/1‑149 (MQ=255)
                                                         cGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcggc                                                                                        <  4:117251/147‑1 (MQ=255)
                                                         cGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGgcgc                                                                                      >  4:349386/1‑149 (MQ=255)
                                                          ggCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGgcgc                                                                                      <  1:98123/148‑1 (MQ=255)
                                                          ggCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCAGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCATCGgcgc                                                                                      >  4:69160/1‑148 (MQ=255)
                                                           gCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGcgcg                                                                                     >  7:73629/1‑148 (MQ=255)
                                                               ttCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTg                                                                                >  7:155503/1‑149 (MQ=255)
                                                                tCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGc                                                                               >  7:289245/1‑149 (MQ=255)
                                                                 cAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGc                                                                               <  3:349208/148‑1 (MQ=255)
                                                                 cAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGc                                                                               <  3:277085/148‑1 (MQ=255)
                                                                 cAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGc                                                                               <  1:375821/148‑1 (MQ=255)
                                                                 cAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCg                                                                              >  1:342056/1‑149 (MQ=255)
                                                                        gCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTtc                                                                         <  1:192814/147‑1 (MQ=255)
                                                                          gTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTtcgtcg                                                                     <  4:367704/149‑1 (MQ=255)
                                                                             tGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGc                                                                  >  5:159406/1‑149 (MQ=255)
                                                                              gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGc                                                                  <  2:27437/148‑1 (MQ=255)
                                                                              gCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCt                                                                 >  7:322353/1‑149 (MQ=255)
                                                                                 gCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACAGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTcca                                                              >  1:275802/1‑149 (MQ=255)
                                                                                     aTGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTccacca                                                           >  3:290659/1‑148 (MQ=255)
                                                                                       gTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg                                                                                                 >  5:52943/1‑108 (MQ=255)
                                                                                       gTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATg                                                                                                 <  6:52943/108‑1 (MQ=255)
                                                                                       gTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACaa                                                        >  5:154322/1‑149 (MQ=255)
                                                                                          cTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCa                                                     <  8:155503/149‑1 (MQ=255)
                                                                                             cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACgg                                                  >  2:91557/1‑149 (MQ=255)
                                                                                             cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACgg                                                  >  4:107031/1‑149 (MQ=255)
                                                                                             cccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACg                                                   <  4:290811/148‑1 (MQ=255)
                                                                                              ccGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGt                                                 <  5:26936/149‑1 (MQ=255)
                                                                                               cGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTACGTCGTGCTCCACCACAATCAc                                                    <  8:33397/145‑1 (MQ=255)
                                                                                               cGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTACGTCGTGCTCCACCACAATCAc                                                    >  7:33397/1‑145 (MQ=255)
                                                                                                  acAACGACTCCGGCACCGCCATAATCGCTTCCACCGTACCTAATGCCACAACTACCCCGCCCTGACAACCTCCGCCCGGGCAAAAGTCGATCACAGTGTCAGAGGCCCCAATTGCGTCTTAGAGGTGCGCCACCACCATCACGGAAtaa                                             >  4:46316/2‑147 (MQ=255)
                                                                                                    aaCGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAcc                                           >  8:188963/1‑149 (MQ=255)
                                                                                                    aaCGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGACTTCGTCGTGCTCCACCACAATCACGGTATTAcc                                           >  2:248804/1‑149 (MQ=255)
                                                                                                         cTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGAt                                      <  8:322353/149‑1 (MQ=255)
                                                                                                          tCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGAt                                      >  2:271592/1‑148 (MQ=255)
                                                                                                            cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGAt                                      <  1:271592/146‑1 (MQ=255)
                                                                                                            cGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACAACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGGCAGCGTCGCGAATTGCGTCTTCGTCGTGCTACACCACAATCACGGTATTACCGAGATc                                     <  3:69160/147‑1 (MQ=255)
                                                                                                                  cGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGAt                             <  3:332187/149‑1 (MQ=255)
                                                                                                                  cGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGAt                             <  3:107031/149‑1 (MQ=255)
                                                                                                                   gCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATg                            <  6:159406/149‑1 (MQ=255)
                                                                                                                   gCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAAATg                            <  6:154322/149‑1 (MQ=255)
                                                                                                                          tCGCTTCCAGCGGACCTTCTGCGCCCACTTCACCGCCGTCAACACCTGCGCCCGGGCCAATGTCGCTCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCCCCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGc                     >  6:104503/1‑149 (MQ=255)
                                                                                                                          tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTc                                                                                                          >  7:322329/1‑64 (MQ=255)
                                                                                                                          tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTc                                                                                                          <  8:322329/64‑1 (MQ=255)
                                                                                                                          tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGc                     <  7:84891/149‑1 (MQ=255)
                                                                                                                          tCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGc                     <  3:41723/149‑1 (MQ=255)
                                                                                                                              ttCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCCCCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAc                 >  8:153884/1‑149 (MQ=255)
                                                                                                                                ccAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAcc                <  1:91557/148‑1 (MQ=255)
                                                                                                                                  aGCGGACCTTCTGCGACCACTTCACCGCCGTGCACACCTGCGCCCGGGCCAATGTCGATCACCTGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTAATACCGCGCGCGCGCAGATGGATAAGCGTACCCaa             >  8:105238/1‑149 (MQ=255)
                                                                                                                                   gCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCaa             >  8:220818/1‑148 (MQ=255)
                                                                                                                                    cGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCACGGGCCAATCTCGCTCCCATGGTCAGCGGCGCGAATTGCGGCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACa           <  2:286728/149‑1 (MQ=255)
                                                                                                                                         cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGgcgc      <  1:248804/149‑1 (MQ=255)
                                                                                                                                          ttCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCt     >  3:80162/1‑149 (MQ=255)
                                                                                                                                              gCGACCACTTCACCGCCGTGAACACATTAGCCCGGGACAATGTCGATCACATGGTCAGAGCCGCTAATTGCGTCTTCGTCGTTCTCAAACACAATCAAGGTATTAGCGAGATCGAGCAGATGTATAAGCGTACCAAACAGTCGCTCGt  >  8:361340/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
GCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGT  >  NC_000913/4272012‑4272301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑