Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 605,025 C→G 5.9% D134H (GAT→CAT)  nfsB ← dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913605,0250CG 5.9% 231.5 / 4.6 68D134H (GAT→CAT) nfsBdihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
Reads supporting (aligned to +/- strand):  ref base C (35/29);  new base G (2/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCAC  >  NC_000913/604879‑605171
                                                                                                                                                  |                                                                                                                                                  
gCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatcat                                                                                                                                                  >  2:391964/1‑149 (MQ=255)
gCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatcat                                                                                                                                                  <  6:168063/149‑1 (MQ=255)
  cTTTCTCTTTCAGACCAAATTCTGCATCGCGGATGGCGGCGTCAAACCCTTAGATCTTTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGc                                                                                                                                                <  2:93797/149‑1 (MQ=255)
    ttCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATg                                                                                                                                                 >  8:371580/1‑146 (MQ=255)
      ctctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGa                                                                                                                                             <  1:18138/148‑1 (MQ=255)
         tttCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGAt                                                                                                                                            <  6:163217/146‑1 (MQ=255)
           tCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGag                                                                                                                                            <  1:192779/144‑2 (MQ=255)
           tCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGag                                                                                                                                            >  2:192779/1‑143 (MQ=255)
           tCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTa                                                                                                                                       >  7:299146/1‑149 (MQ=255)
               aCCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAACCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGt                                                                                                                                   >  6:375784/1‑149 (MQ=255)
                 cAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTg                                                                                                                                  >  2:107371/1‑148 (MQ=255)
                 cAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTg                                                                                                                                  >  7:351860/1‑148 (MQ=255)
                 cAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                                                                                 <  8:351860/149‑1 (MQ=255)
                 cAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                                                                                 <  5:531/149‑1 (MQ=255)
                          cATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAg                                                                                                                          <  6:76573/147‑1 (MQ=255)
                           aTCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAACCCTGTTTTGCCATCCACTCTGCATCATCATGCAGCTCTTTACGGTGCATATCAGCg                                                                                                                        >  2:19725/1‑148 (MQ=255)
                           aTCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCga                                                                                                                       <  1:68094/149‑1 (MQ=255)
                            tCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaa                                                                                                                      <  8:150791/149‑1 (MQ=255)
                               aGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaagaa                                                                                                                   >  3:225176/1‑149 (MQ=255)
                                        gcttCAAAACCTTAGATGGGTACCGCGTCCAGAACCAGAGCCGACACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGAcc                                                                                                          <  7:370399/146‑1 (MQ=255)
                                         cgTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCt                                                                                                         <  1:107371/149‑1 (MQ=255)
                                         cgTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCt                                                                                                         <  8:299146/149‑1 (MQ=255)
                                           tCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCtt                                                                                                        >  8:335152/1‑148 (MQ=255)
                                             aaaaCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTAt                                                                                                     <  4:48924/149‑1 (MQ=255)
                                              aaaCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATc                                                                                                    <  4:218202/149‑1 (MQ=255)
                                               aaCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCg                                                                                                   >  2:18596/1‑149 (MQ=255)
                                                aCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                                                                  <  4:200680/149‑1 (MQ=255)
                                                       aTGGGTACCGCGTCCAGACCCAGAGCCGCTACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGc                                                                                           >  8:164070/1‑149 (MQ=255)
                                                       aTGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGc                                                                                           >  2:191536/1‑149 (MQ=255)
                                                           gTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTc                                                                                       <  7:371580/149‑1 (MQ=255)
                                                               cgcgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCtt                                                                                   >  2:344626/1‑149 (MQ=255)
                                                                            agagCCGCCACGCCGAGCATGACTTTACCGACGTTGAGATAAACCTGTGTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTCTCGGTCGCGGCTTTCGCTTCCGGCGGGGCaaa                                                                      <  6:15941/149‑1 (MQ=255)
                                                                                  gccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGAt                                                                                                                                            <  2:253730/73‑1 (MQ=255)
                                                                                  gccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGAt                                                                                                                                            >  1:253730/1‑73 (MQ=255)
                                                                                  gccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGcc                                                                <  6:229068/149‑1 (MQ=255)
                                                                                   ccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCGGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCa                                                               >  8:331070/1‑149 (MQ=255)
                                                                                      cGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCg                                                            <  1:344626/149‑1 (MQ=255)
                                                                                       gCCGAGCAGGAAGTTACCGAATTTGAGATAAACCTGTATTTCCATCCACTCTGCATCATCATGCAGATATTTACGGTGCATATCAGCGAAGAACTTGATACCTTTATCGTTCGCGGCTTTCGCTTCCGGAGTGGCAAAGCGGCCAGCgg                                                           >  8:233197/1‑149 (MQ=255)
                                                                                         cGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCCGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCa                                                         <  2:241550/149‑1 (MQ=255)
                                                                                         cGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCa                                                         >  5:294342/1‑149 (MQ=255)
                                                                                              aGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTc                                                    <  7:335152/149‑1 (MQ=255)
                                                                                                    ttACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCTTCTTCCTGGt                                               >  6:350704/1‑148 (MQ=255)
                                                                                                      aCCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTcaa                                            >  4:211662/1‑149 (MQ=255)
                                                                                                          aCGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCaacaac                                        >  6:321415/1‑149 (MQ=255)
                                                                                                          aCGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCaacaac                                        >  2:109756/1‑149 (MQ=255)
                                                                                                               gagaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGc                                    <  3:109647/148‑1 (MQ=255)
                                                                                                                  aTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCtt                                  >  7:74581/1‑147 (MQ=255)
                                                                                                                  aTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCa                                <  5:321415/149‑1 (MQ=255)
                                                                                                                  aTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCa                                <  1:191536/149‑1 (MQ=255)
                                                                                                                  aTAAACATGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCtt                                  >  2:264166/1‑147 (MQ=255)
                                                                                                                      aCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGc                              >  8:223513/1‑147 (MQ=255)
                                                                                                                        cTGTTTTGCAATCCACTCTGCATCATCATGCAGATCTATACGGTTCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCATGGTCAACCACCAGCTTCAGCCAGa                          >  6:343916/1‑149 (MQ=255)
                                                                                                                         tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCttt                                                                                        <  4:144936/86‑1 (MQ=255)
                                                                                                                         tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCttt                                                                                        >  3:144936/1‑86 (MQ=255)
                                                                                                                         tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGAc                         <  1:264166/149‑1 (MQ=255)
                                                                                                                          gTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACa                        >  1:113802/1‑149 (MQ=255)
                                                                                                                           ttttGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACAt                       >  8:245224/1‑149 (MQ=255)
                                                                                                                               gCCATCCACTCTGCATCATCATTCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCTCTTCCTGCGGGGCAAAGCGGCCATCGGCATCTTCCTTGTCAACAACCATCTTCAGCCAGACATCGt                    <  7:233197/148‑1 (MQ=255)
                                                                                                                                  aTCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCtt                                  >  3:199292/1‑131 (MQ=255)
                                                                                                                                  aTCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCtt                                  <  4:199292/131‑1 (MQ=255)
                                                                                                                                  aTCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCAt                >  3:64652/1‑149 (MQ=255)
                                                                                                                                   tCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATc               >  4:358496/1‑149 (MQ=255)
                                                                                                                                   tCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATc               >  3:266741/1‑149 (MQ=255)
                                                                                                                                      aCTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATcgcg            >  7:92972/1‑149 (MQ=255)
                                                                                                                                       atctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGt                                                                            <  1:187735/83‑1 (MQ=255)
                                                                                                                                       atctGCATGATGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGt                                                                            >  2:187735/2‑84 (MQ=255)
                                                                                                                                        tctGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATc                      <  7:275149/137‑1 (MQ=255)
                                                                                                                                        tctGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATc                      >  8:275149/1‑137 (MQ=255)
                                                                                                                                          tGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGttt        >  3:211369/1‑149 (MQ=255)
                                                                                                                                           gCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGtttt       >  5:369667/1‑149 (MQ=255)
                                                                                                                                            catcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGTGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGttttt      <  7:279943/149‑1 (MQ=255)
                                                                                                                                            catcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGt          <  4:225176/145‑1 (MQ=255)
                                                                                                                                             atcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCTTCCATCGCGGTTTTTg     >  5:341411/1‑149 (MQ=255)
                                                                                                                                             atcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGt                    >  7:384404/1‑134 (MQ=255)
                                                                                                                                             atcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGt                    <  8:384404/134‑1 (MQ=255)
                                                                                                                                             atcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTg     >  6:73986/1‑149 (MQ=255)
                                                                                                                                                atcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGcac  <  4:266741/149‑1 (MQ=255)
                                                                                                                                                atcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGca   <  7:164070/148‑1 (MQ=255)
                                                                                                                                                 tcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGca   >  1:6451/1‑147 (MQ=255)
                                                                                                                                                 tcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTGTTGca   >  4:308354/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCAC  >  NC_000913/604879‑605171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: