Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	44,943	T→C	5.0%	L255P (CTG→CCG)	fixC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	44,943	0	T	C	5.0%	245.9 / 2.8	79	L255P (CTG→CCG)	fixC	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base C (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATGCCGCC  >  NC_000913/44797‑45091                                                                                                                                                   |                                                                                                                                                     gggggCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTgg                                                                                                                                                    <  4:51862/149‑1 (MQ=255)  ggggCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACCCCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTgg                                                                                                                                                    >  4:36345/1‑148 (MQ=255)      cggcTTGCCTGTTTGCGGGAGCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGAt                                                                                                                                               <  2:380659/149‑1 (MQ=255)             ccTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAAc                                                                                                                                        <  5:383955/149‑1 (MQ=255)                gTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAg                                                                                                                                      >  2:174992/1‑148 (MQ=255)                 tttGCGGGATCACCCACCGATGGCCTGATGGGCGGCTGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGc                                                                                                                                     <  4:303599/148‑1 (MQ=255)                      gggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCCATGAAACCACCCTGTCGCTGGGGCTGGTTTGTGGTTGGCATCATCTGCCTGACGCGAAAACATCGGTGCCGCAAATGCTGGAAGATTTCAAACCGCATCCgg                                                                                                                               >  3:79681/1‑149 (MQ=255)                      gggATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCgg                                                                                                                               >  2:80820/1‑149 (MQ=255)                              ccaccGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGGAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCa                                                                                                                       <  7:124302/149‑1 (MQ=255)                              ccaccGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGc                                                                                                                                     >  8:61357/1‑135 (MQ=255)                              ccaccGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGc                                                                                                                                     <  7:61357/135‑1 (MQ=255)                              ccaccGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCa                                                                                                                       >  4:162262/1‑149 (MQ=255)                              ccaccGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCa                                                                                                                       >  3:200800/1‑149 (MQ=255)                                     tGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGa                                                                                                                <  6:249645/149‑1 (MQ=255)                                         cTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACCCCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATcgc                                                                                                            >  8:141940/1‑149 (MQ=255)                                         cTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATcgc                                                                                                            >  7:207390/1‑149 (MQ=255)                                            aTGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATcgcg                                                                                                           <  8:77599/147‑1 (MQ=255)                                               ggcggcggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCgcgggcg                                                                                                       <  1:174992/148‑1 (MQ=255)                                                  ggcggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGggcggc                                                                                                     >  3:123219/1‑147 (MQ=255)                                                    cggcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGc                                                                                                 <  7:141940/149‑1 (MQ=255)                                                      gcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTc                                                                                                                                            >  4:154261/1‑104 (MQ=255)                                                      gcTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTc                                                                                                                                            <  3:154261/104‑1 (MQ=255)                                                       cTTCCTTTATA‑CAATGAAAACACCCTGTCGCGGGGGCGGGGTTGGGGGTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCtggt                                                                                             <  1:90059/149‑1 (MQ=255)                                                        ttCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCtggt                                                                                             >  6:76405/1‑149 (MQ=255)                                                           cTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCtggtgg                                                                                           >  8:350793/1‑148 (MQ=255)                                                           cTTTATACCAATGAAAACACCCTGTCCCTGGGGCTGGGTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCtggtgg                                                                                           <  5:139075/148‑1 (MQ=255)                                                              tataCCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTGGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGaa                                                                                         <  6:252926/147‑1 (MQ=255)                                                              tataCCAATGAAAACACCCTGTCGCTGGGGCTGGGTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGCTCGCGGGCGGCAAGCTGGTGGaa                                                                                         <  5:337788/147‑1 (MQ=255)                                                               ataCCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTCCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCAGTTGCACCGCTGATCGCTGGCGGCAAGCTGGTGGAataa                                                                                      >  1:332595/1‑148 (MQ=255)                                                                taCCAATGAAAACACCCTGTCGCTGGGGCGGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAatat                                                                                      <  4:59432/148‑1 (MQ=255)                                                                 aCCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGAATCATCTGCATGACGCGAAAAAATCGTTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGAACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTc                                                                                    >  4:328124/1‑149 (MQ=255)                                                                 aCCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTATGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTc                                                                                    >  2:263621/1‑149 (MQ=255)                                                                  ccAATGAAAACACCCTGTCGCGGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTcc                                                                                   <  1:323097/149‑1 (MQ=255)                                                                   cAATGAACACACCCACTCGCTGCGGCTGGTTTGTGGATTGCATCATCTGCATGACGCGAAAAAATAGGTGCCGCAAATGCTGGAAGATTTCAAACAGAATCCTGACGTTGCACCGCAGATCGCGGGCTGCAATCTCGTGGAATATTCCg                                                                                  >  6:338451/1‑149 (MQ=255)                                                                    aaTGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCCGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCg                                                                                  >  2:183176/1‑148 (MQ=255)                                                                    aaTGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCCGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGc                                                                                 >  2:177674/1‑149 (MQ=255)                                                                      tGAAAACCCCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCCCCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTc                                                                               >  4:347899/1‑149 (MQ=255)                                                                        aaaaCACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCa                                                                              <  3:162262/148‑1 (MQ=255)                                                                          aaCACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACg                                                                            >  4:116488/1‑148 (MQ=255)                                                                               ccTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAat                                                                                        <  4:233202/131‑1 (MQ=255)                                                                               ccTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAat                                                                                        >  3:233202/1‑131 (MQ=255)                                                                               ccTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGc                                                                      >  3:26539/1‑149 (MQ=255)                                                                               ccTGTCGCTCGGGCTGGTTTAAGGTTTGCATCATCTGCATGACCCGAAAAAATCCGTGCCGCAAATGCTCGAAGATTTCAAACAGCATCCGGCAGTTGCACCGCTGATCGCGTGCGGCAAGCTTGTGGAATATTCCGCTCACTTAGTga                                                                      >  4:42605/1‑148 (MQ=255)                                                                                   tCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCgg                                                                   <  5:76405/148‑1 (MQ=255)                                                                                      cTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGc                                                               <  4:26539/149‑1 (MQ=255)                                                                                         gggCTGGTTTGTGGTCTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAgg                                                            <  1:263621/149‑1 (MQ=255)                                                                                           gCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCa                                                          >  2:170740/1‑149 (MQ=255)                                                                                            cTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTc                                                                               <  5:170975/127‑1 (MQ=255)                                                                                            cTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTc                                                                               >  6:170975/1‑127 (MQ=255)                                                                                             tggtttgtggtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCCGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATc                                                        <  1:183176/149‑1 (MQ=255)                                                                                               gtttgtggtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCCGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATc                                                        <  1:177674/147‑1 (MQ=255)                                                                                                  tgtggtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACa                                                    <  8:207390/148‑1 (MQ=255)                                                                                                   gtggtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACAtg                                                  >  4:96295/1‑149 (MQ=255)                                                                                                     ggtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGCGCCGGAAGCAGGCATCAACAtgct                                                >  4:158115/1‑149 (MQ=255)                                                                                                      gtttgCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACAtgctg                                               <  1:170740/149‑1 (MQ=255)                                                                                                          gCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCg                                            >  7:26462/1‑148 (MQ=255)                                                                                                            atcatcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATAtt                                                                                     <  2:177944/105‑1 (MQ=255)                                                                                                            atcatcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAg                                         >  7:51681/1‑149 (MQ=255)                                                                                                              catcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgtt                                       >  4:262950/1‑149 (MQ=255)                                                                                                               atcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgttg                                      <  3:36345/149‑1 (MQ=255)                                                                                                                tcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACAtgct                                                >  2:314770/1‑138 (MQ=255)                                                                                                                tcTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACAtgct                                                <  1:314770/138‑1 (MQ=255)                                                                                                                    cATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgttggt                                    <  1:220335/146‑1 (MQ=255)                                                                                                                    cATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgttgg                                     <  6:206868/145‑1 (MQ=255)                                                                                                                     aTGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTtggttggt                                >  7:26404/1‑149 (MQ=255)                                                                                                                        aCGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGAc                             <  3:262950/149‑1 (MQ=255)                                                                                                                          gcgAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACgg                           >  5:262478/1‑149 (MQ=255)                                                                                                                           cgAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGc                          >  2:151466/1‑149 (MQ=255)                                                                                                                            gAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCg                         >  3:3180/1‑149 (MQ=255)                                                                                                                             aaaaaaTCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCg                         >  7:361620/1‑148 (MQ=255)                                                                                                                               aaaaTCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTat                      <  4:341381/149‑1 (MQ=255)                                                                                                                               aaaaTCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTat                      >  3:45916/1‑149 (MQ=255)                                                                                                                                     ggTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTattgatt                 <  1:151466/148‑1 (MQ=255)                                                                                                                                         ccGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTa                       <  4:96999/138‑1 (MQ=255)                                                                                                                                         ccGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTa                       >  3:96999/1‑138 (MQ=255)                                                                                                                                         ccGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCgg            <  3:42605/149‑1 (MQ=255)                                                                                                                                           gCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGt           >  3:220591/1‑148 (MQ=255)                                                                                                                                             aaaTGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGAt        <  4:200800/149‑1 (MQ=255)                                                                                                                                                  cTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATgccgc   <  6:262478/149‑1 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgttggt                                    <  7:201130/115‑1 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAgttggt                                    >  8:201130/1‑115 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATgccgcc  >  7:144638/1‑149 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATgccgcc  >  3:93527/1‑149 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATgccgcc  >  8:361658/1‑149 (MQ=255)                                                                                                                                                   tGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATgccgcc  >  1:11337/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GGGGGCGGCTTGCCTGTTTGCGGGATCACCCACCGATGGCCTGATGGGCGGCGGCTTCCTTTATACCAATGAAAACACCCTGTCGCTGGGGCTGGTTTGTGGTTTGCATCATCTGCATGACGCGAAAAAATCGGTGCCGCAAATGCTGGAAGATTTCAAACAGCATCCGGCCGTTGCACCGCTGATCGCGGGCGGCAAGCTGGTGGAATATTCCGCTCACGTAGTGCCGGAAGCAGGCATCAACATGCTGCCGGAGTTGGTTGGTGACGGCGTATTGATTGCCGGTGATGCCGCC  >  NC_000913/44797‑45091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑