Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,825 T→C 5.5% M259T (ATG→ACG)  ydaN → putative Zn(II) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,8250TC 5.5% 233.1 / 2.0 77M259T (ATG→ACG) ydaNputative Zn(II) transporter
Reads supporting (aligned to +/- strand):  ref base T (37/33);  new base C (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTTGCCAGTGAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGATTTTCAATTTTTTGTAT  >  NC_000913/1408678‑1408972
                                                                                                                                                   |                                                                                                                                                   
gTCTTGCCAGTGAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATg                                                                                                                                                    >  3:199338/1‑149 (MQ=255)
gTCTTGCCAGTGAACGTTTGC‑‑TGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCa                                                                                                                                                  <  3:229232/149‑1 (MQ=255)
     gCCAGTGAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCAGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCa                                                                                                                                                  <  4:128889/146‑1 (MQ=255)
          tGAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAAtt                                                                                                                                          >  7:276717/1‑149 (MQ=255)
           gAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAAtt                                                                                                                                          >  3:343028/1‑148 (MQ=255)
               gTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGCTGGCGGATGAAATCGCTCAGGTGAGGCAGGAAAATTTAggt                                                                                                                                     >  2:378319/1‑147 (MQ=255)
                    ccGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCtcgtcg                                                                                                                                >  5:47750/1‑149 (MQ=255)
                      gTGGATGAGCGATGACCAACGCCGTCGGGTGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTa                                                                                                                              >  5:285777/1‑149 (MQ=255)
                            gAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGAACGGACTGGCGTGATGGCGGAAGAAATCGCTCAGGTGATGCAGGAAATTTTAGCTCGTCGTACCgata                                                                                                                        >  1:127487/1‑149 (MQ=255)
                                 aTGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATg                                                                                                                   <  1:378319/149‑1 (MQ=255)
                                    aCCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCg                                                                                                                >  1:18353/1‑149 (MQ=255)
                                     ccAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCtcgtc                                                                                                                                 >  2:246556/1‑131 (MQ=255)
                                     ccAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCtcgtc                                                                                                                                 <  1:246556/131‑1 (MQ=255)
                                      cAACGCCGTCGGATGTAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGtt                                                                                                              <  5:138075/149‑1 (MQ=255)
                                       aaCGACGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACTAAATCGACGCCTGTATAGCACGGACTGGGGTGATGGCGGATGAAATCGCTCAGGTTATGCAGGAAAATTTAGATCGTCTTACCTATCCAATGTCGtt                                                                                                              >  6:84496/1‑148 (MQ=255)
                                        aCGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGtt                                                                                                              >  3:162783/1‑147 (MQ=255)
                                        aCGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGtt                                                                                                              <  4:162783/147‑1 (MQ=255)
                                          gCCGGCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATg                                                                                                          <  6:46632/149‑1 (MQ=255)
                                             gTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGc                                                                                                        >  2:284711/1‑148 (MQ=255)
                                                     cAGGATATTGCCGATCGCCTTGGGCTCGGCCTTGACGAAATCGACGACTGGATAGAACGGACTGGCGTGATGGCGGATGAAATCGATCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCtt                                                                                               >  4:40671/1‑149 (MQ=255)
                                                     cAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCtt                                                                                               <  2:18353/149‑1 (MQ=255)
                                                     cAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCtt                                                                                               <  4:217899/149‑1 (MQ=255)
                                                      aGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCttt                                                                                              >  5:96724/1‑149 (MQ=255)
                                                      aGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCttt                                                                                              <  6:285777/149‑1 (MQ=255)
                                                       ggATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCtttt                                                                                             >  5:109442/1‑149 (MQ=255)
                                                        gATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCtttt                                                                                             >  7:405667/1‑148 (MQ=255)
                                                         atatTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTa                                                                                           <  8:405667/149‑1 (MQ=255)
                                                             tcccGATCGCCTTGGGCGCGGCCATGACGAAATCGACGCCAGAATAGCACGGACAGGCGTGATCCCGGAAGAAAACGCTCACGTGAAGCAGCAAAATTTACCTCGTCGTACCTATACAATGTCGTTAATGTCAATGGTCTTTATAcc                                                                                         >  6:257548/3‑147 (MQ=255)
                                                                 gATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGtt                                                                                                              <  4:125672/122‑1 (MQ=255)
                                                                 gATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGtt                                                                                                              >  3:125672/1‑122 (MQ=255)
                                                                       cTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCt                                                                             <  3:92272/149‑1 (MQ=255)
                                                                        ttGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCt                                                                             >  2:105549/1‑148 (MQ=255)
                                                                            gcgcGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCg                                                                        <  5:175456/149‑1 (MQ=255)
                                                                             cgcgGCCTTGACGAAATCGCCGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGAAGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCgg                                                                       <  8:238968/149‑1 (MQ=255)
                                                                              gcgGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCggg                                                                      <  6:96724/149‑1 (MQ=255)
                                                                                  ccTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGACGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTAt                                                                  >  8:118395/1‑149 (MQ=255)
                                                                                   cTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTAtt                                                                 >  8:15264/1‑149 (MQ=255)
                                                                                   cTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGACGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCttt                                                                                              <  5:380446/120‑1 (MQ=255)
                                                                                   cTTGACGAAATCGACGCCTGTATAGCACGGAATGGCGTGATGGCGGATGAAATCGCTCAGGTGACGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCttt                                                                                              >  6:380446/1‑120 (MQ=255)
                                                                                      gACGAAATCGACGCCTGTATATCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGTTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTg                                                               <  1:53717/148‑1 (MQ=255)
                                                                                      gACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGTTGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTg                                                               <  4:199338/148‑1 (MQ=255)
                                                                                      gACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTg                                                               <  3:331215/148‑1 (MQ=255)
                                                                                      gACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTg                                                               <  4:286487/148‑1 (MQ=255)
                                                                                         gAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGt                                                           >  8:165823/1‑149 (MQ=255)
                                                                                             tCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCtata                                                                                                                        <  6:198575/84‑1 (MQ=255)
                                                                                             tCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCtata                                                                                                                        >  5:198575/1‑84 (MQ=255)
                                                                                               gACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAg                                                                                                                                                 <  6:346240/57‑1 (MQ=255)
                                                                                               gACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAg                                                                                                                                                 >  5:346240/1‑57 (MQ=255)
                                                                                               gACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCt                                                     <  1:105549/149‑1 (MQ=255)
                                                                                                 cGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtg                                                   <  6:47750/149‑1 (MQ=255)
                                                                                                  gCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtgg                                                  <  6:109442/149‑1 (MQ=255)
                                                                                                   ccTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtggt                                                 <  1:284711/149‑1 (MQ=255)
                                                                                                    cTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtggtg                                                <  4:130410/149‑1 (MQ=255)
                                                                                                    cTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtggtgg                                               >  8:230176/1‑149 (MQ=255)
                                                                                                     tGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCt                                                     <  2:97321/143‑1 (MQ=255)
                                                                                                     tGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCt                                                     >  1:97321/1‑143 (MQ=255)
                                                                                                      gTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTtggtgg                                               >  2:338092/1‑148 (MQ=255)
                                                                                                         taGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATc                                           >  4:78844/1‑149 (MQ=255)
                                                                                                           gCACGGACTGGCGTGATGGTGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGAACGGGTTATTTGGCGTCAACCTTGGTGGGATccc                                         >  7:24551/1‑149 (MQ=255)
                                                                                                           gCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATccc                                         >  7:7797/1‑149 (MQ=255)
                                                                                                           gCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATccc                                         >  4:146360/1‑149 (MQ=255)
                                                                                                               ggACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTggc                                     >  8:179324/1‑149 (MQ=255)
                                                                                                                  cTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTggcggc                                  >  5:77131/1‑149 (MQ=255)
                                                                                                                        tGATGGCGGATGAAATAGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTgg                            <  3:40671/149‑1 (MQ=255)
                                                                                                                               ggATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACATATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCg                     >  5:308726/1‑149 (MQ=255)
                                                                                                                                gATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCg                     >  8:278881/1‑148 (MQ=255)
                                                                                                                                  tGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGAt                  <  7:15264/149‑1 (MQ=255)
                                                                                                                                    aaaTCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACGTTTCTGACCTGGTTATTTGGCGTCAAGCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGAtt                 <  7:165823/148‑1 (MQ=255)
                                                                                                                                     aaTCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGAtttt               >  8:30651/1‑149 (MQ=255)
                                                                                                                                      aTCGCTCAGGTGATGCAGGAAAATTTAGCTCGACGTACCTATACAATGTCGTTGATGGCAATGGTCTATTTACGCAGTACCTTTCTGACCGGGATATTTGGAGTCAACATAGGTTGTATCCCTCGAGGCGGGTGGCAATTAGGAtttt               >  2:293417/1‑148 (MQ=255)
                                                                                                                                       tgggtgaGGTGATTCAGGAAATTTTTGCTGCTCGTTCGTATACATTGTAGTTGATGGCAATGGTGTTTTTACCCAGTAGCTTTCTGACCGGGTTATTTGGCGTCAACGTTGGTGGGTTGCCTGGCGGGGGGGGGCTATTCGGATTTTc              <  5:257548/142‑1 (MQ=255)
                                                                                                                                           tCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGATTTTCAAttt         >  7:324386/1‑149 (MQ=255)
                                                                                                                                             aGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGcggcgg                                <  4:140629/124‑1 (MQ=255)
                                                                                                                                             aGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGcggcgg                                >  3:140629/1‑124 (MQ=255)
                                                                                                                                             aGGTGACGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGATTTTCAAttttt       <  7:118395/149‑1 (MQ=255)
                                                                                                                                                  aTGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGATTTTCAATTTTTTGTa   <  1:338092/148‑1 (MQ=255)
                                                                                                                                                  aTGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCACTGGCGGCGGGTGGCAATTCGGATTTTCAATTTTTTGTAt  >  3:232943/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCTTGCCAGTGAACGTTTGCCGTGGATGAGCGATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAAATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAGGAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGTACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAATTCGGATTTTCAATTTTTTGTAT  >  NC_000913/1408678‑1408972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: