Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,739,891 G→A 5.6% intergenic (‑93/‑20) ydhB ← / → ydhC LysR family putative transcriptional regulator/putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,739,8910GA 5.6% 228.8 / 2.9 71intergenic (‑93/‑20)ydhB/ydhCLysR family putative transcriptional regulator/putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base A (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

AACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTCTGCTG  >  NC_000913/1739744‑1740037
                                                                                                                                                   |                                                                                                                                                  
aaCTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTga                                                                                                                                                   <  4:174489/149‑1 (MQ=255)
        ttACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGAtt                                                                                                                                             >  2:176828/1‑147 (MQ=255)
           cgcgCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTaga                                                                                                                                         <  1:48570/148‑1 (MQ=255)
              gcTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAat                                                                                                                                     <  3:193945/149‑1 (MQ=255)
                  ccGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATAc                                                                                                                                 <  7:180809/149‑1 (MQ=255)
                  ccGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATAc                                                                                                                                 <  4:41272/149‑1 (MQ=255)
                    gCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACAt                                                                                                                               >  4:255391/1‑149 (MQ=255)
                        caacaaCTTCAAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTATTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAAAAACACTCCCAACACAATGCGGCGCTGTATAATTGAGAGAATAAGAGAAAATACAAGCaa                                                                                                                           >  8:260483/1‑149 (MQ=255)
                         aacaacTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAAc                                                                                                                          >  6:319517/1‑149 (MQ=255)
                          acaacTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGc                                                                                                                             <  7:242412/145‑1 (MQ=255)
                          acaacTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGc                                                                                                                             >  8:242412/1‑145 (MQ=255)
                          acaacTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGGTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTAAGAGATTTAGAGAATATACATGCAAcc                                                                                                                         >  6:209827/1‑149 (MQ=255)
                           caacTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCt                                                                                                                        >  4:236572/1‑149 (MQ=255)
                                  gAGTGAATATTCTGACCACATAGTCTGCCTGCAAACTTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGaaaa                                                                                                                 <  3:232379/149‑1 (MQ=255)
                                  gAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGaa                                                                                                                   >  7:243721/1‑147 (MQ=255)
                                  gAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTAATCAAATATTACCGTTTCACAACACTACTTTCACTCCCTACACTTTTCGGCGGTGTTTAATTGAGAGAGTTAGAGAATATACATGCACCCTGGGaaaa                                                                                                                 <  6:340522/149‑1 (MQ=255)
                                  gAGTGAATATTCTGACCACATAGTCAGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGaaa                                                                                                                  <  1:176828/148‑1 (MQ=255)
                                     tGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGAt                                                                                                              <  7:260483/149‑1 (MQ=255)
                                       aaTATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTTATTTCACTCCCTACACTTTGAGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGAttt                                                                                                            >  8:315218/1‑149 (MQ=255)
                                       aaTATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGAttt                                                                                                            <  3:122910/149‑1 (MQ=255)
                                             cTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGt                                                                                                       <  6:226675/148‑1 (MQ=255)
                                              tGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTAAGAGATTTAGAGAATATACATGCACCCTGGGAAAAGATTTTTAGTct                                                                                                     >  2:135985/1‑149 (MQ=255)
                                                 ccACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGTCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTctggc                                                                                                  >  3:120913/1‑149 (MQ=255)
                                                         tCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGt                                                                                          >  7:344057/1‑149 (MQ=255)
                                                             cctgcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGa                                                                                      <  2:386226/149‑1 (MQ=255)
                                                              ctgcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAg                                                                                     >  8:305763/1‑149 (MQ=255)
                                                              ctgcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAg                                                                                     >  1:177079/1‑149 (MQ=255)
                                                               tgcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGc                                                                                    >  1:360530/1‑149 (MQ=255)
                                                                gcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGttt                                                                                        <  2:390787/144‑1 (MQ=255)
                                                                gcAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGttt                                                                                        >  1:390787/1‑144 (MQ=255)
                                                                 cAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGc                                                                                    <  1:180449/147‑1 (MQ=255)
                                                                 cAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCg                                                                                   >  5:10441/1‑148 (MQ=255)
                                                                  aaaaTTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGt                                                                                  >  1:81552/1‑148 (MQ=255)
                                                                            aaaCCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGtt                                                                                         >  8:35107/1‑131 (MQ=255)
                                                                            aaaCCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGtt                                                                                         <  7:28877/131‑1 (MQ=255)
                                                                            aaaCCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGtt                                                                                         <  7:35107/131‑1 (MQ=255)
                                                                            aaaCCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGtt                                                                                         >  8:28877/1‑131 (MQ=255)
                                                                             aaCCAGTCATCAAATATTACCGTTTCACCACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGGCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCt                                                                      <  2:43511/149‑1 (MQ=255)
                                                                               ccAGTCATGAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGCATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTgg                                                                    <  5:306022/149‑1 (MQ=255)
                                                                                      tcaAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAGCCGa                                                             >  5:29427/1‑149 (MQ=255)
                                                                                      tcaAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGGTTGAGCGTACTCGGTTTTCTGGCAACCGa                                                             <  1:160054/149‑1 (MQ=255)
                                                                                                ccGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATct                                                    <  2:79487/148‑1 (MQ=255)
                                                                                                   tttCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATctgcct                                                <  3:236572/149‑1 (MQ=255)
                                                                                                       acaacaCTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCtt                                            >  2:372122/1‑149 (MQ=255)
                                                                                                         aacaCTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTc                                          >  1:254895/1‑149 (MQ=255)
                                                                                                         aacaCTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTc                                          <  5:319517/149‑1 (MQ=255)
                                                                                                         aacaCTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTc                                          <  7:204694/149‑1 (MQ=255)
                                                                                                         aacaCTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTAAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTc                                          <  1:135985/149‑1 (MQ=255)
                                                                                                          acacTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTAAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTcg                                         <  5:209827/149‑1 (MQ=255)
                                                                                                           cacTAATTTCACTCCCTACACTTTGCGGCGTTGTTTAATTGAGAGATTTAGAGTATATACATGCAACCGGGGAAAAGATTTTTAGTCTGTCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCCACCGATATGTATCTGCCTGCTTTcgc                                        <  2:139263/149‑1 (MQ=255)
                                                                                                            acTTATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTcgcc                                       <  7:315218/149‑1 (MQ=255)
                                                                                                            acTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTcgcc                                       >  4:308413/1‑149 (MQ=255)
                                                                                                              tAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTcgccgc                                     >  5:171483/1‑149 (MQ=255)
                                                                                                              tAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGACTGCTTTcgccgc                                     >  1:183440/1‑149 (MQ=255)
                                                                                                                aTTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCa                                   >  5:289816/1‑149 (MQ=255)
                                                                                                                          cTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGa                         >  6:153292/1‑149 (MQ=255)
                                                                                                                          cTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGAGATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGa                         >  3:177746/1‑149 (MQ=255)
                                                                                                                             cacTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGAc                        <  7:65202/147‑1 (MQ=255)
                                                                                                                               cTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGc                    <  3:255391/149‑1 (MQ=255)
                                                                                                                                  tGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGAGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCAATACAGGCCGACCTGCaaa                 >  7:93828/1‑149 (MQ=255)
                                                                                                                                       cggTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCt            <  8:243721/149‑1 (MQ=255)
                                                                                                                                         gtgtTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGc          <  2:254895/149‑1 (MQ=255)
                                                                                                                                          tgtTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTgg                                                                    <  5:249738/90‑1 (MQ=255)
                                                                                                                                          tgtTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTgg                                                                    >  6:249738/1‑90 (MQ=255)
                                                                                                                                           gtTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACa                               >  7:222187/1‑126 (MQ=255)
                                                                                                                                           gtTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACa                               <  8:222187/126‑1 (MQ=255)
                                                                                                                                              tAATTGAGAGATTTAGAGAATCTACATGCAACCTGGGAAAAGCTTTTTAGTCTGGCTGGCGGGATTGAGCGTACTCGGTGTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTct      <  7:186469/148‑1 (MQ=255)
                                                                                                                                              tAATTGAGAGAGTTAGAGAATATACATGCCTCGTGGGAATTGATTTTTAGTCTGGCTGGCGGGTTGGTTCGTACTCGGTTTTCTGGCAACCGATATTTATCTGCCTGCTTTCGCCGCCATACAGTCCGACCTGCAAACGCCTGCGTct      <  4:120913/148‑1 (MQ=255)
                                                                                                                                                aTTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTctgct   >  2:314708/1‑149 (MQ=255)
                                                                                                                                                aTTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTctgc    <  7:305763/148‑1 (MQ=255)
                                                                                                                                                 ttGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTctgctg  >  2:224869/1‑149 (MQ=255)
                                                                                                                                                 ttGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTctgctg  >  4:174205/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTACCATTACGCGCTACCGCATCAACAACTTCGAGTGAATATTCTGACCACATAGTCTGCCTGCAAAATTTTTGAAACCAGTCATCAAATATTACCGTTTCACAACACTAATTTCACTCCCTACACTTTGCGGCGGTGTTTAATTGAGAGATTTAGAGAATATACATGCAACCTGGGAAAAGATTTTTAGTCTGGCTGGCGGGTTTGAGCGTACTCGGTTTTCTGGCAACCGATATGTATCTGCCTGCTTTCGCCGCCATACAGGCCGACCTGCAAACGCCTGCGTCTGCTG  >  NC_000913/1739744‑1740037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑