Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,849,913 T→C 7.7% A359A (GCT→GCC)  sppA → protease IV (signal peptide peptidase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,849,9130TC 7.7% 147.0 / 3.8 52A359A (GCT→GCC) sppAprotease IV (signal peptide peptidase)
Reads supporting (aligned to +/- strand):  ref base T (28/20);  new base C (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

ATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCAT  >  NC_000913/1849767‑1850061
                                                                                                                                                  |                                                                                                                                                    
aTCAGTTATTACGATTACGCATTGAAACCGCCGGCAGATACCGGTGACAGCATCGCTGTCGTCTTTGCTAATGGCGCAATTATGGATTGCGAGGAAACTCAGGGGAATGTTGGCGGTTATACCACTGCGGCACAAATCCGCGACGCTCg                                                                                                                                                    <  1:100710/149‑1 (MQ=255)
    gTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCtt                                                                                                                                                >  2:90685/1‑149 (MQ=255)
       attacgattacgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGTCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGa                                                                                                                                              <  2:155640/148‑1 (MQ=255)
       attacgattacgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGAc                                                                                                                                             >  2:186291/1‑149 (MQ=255)
           cgattacgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCCCGCCTTGACCCg                                                                                                                                          >  7:59074/1‑148 (MQ=255)
            gattacgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGa                                                                                                                                         >  5:335970/1‑148 (MQ=255)
              ttacgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCCCGCCTTGACCCGaaa                                                                                                                                       <  8:59074/148‑1 (MQ=255)
                acgCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGt                                                                                                                                     <  7:201952/148‑1 (MQ=255)
                     ttGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGc                                                                                                                               >  3:237404/1‑149 (MQ=255)
                         aaaCGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGAtt                                                                                                                           <  6:34622/149‑1 (MQ=255)
                                  cAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCgt                                                                                                                  >  7:397114/1‑149 (MQ=255)
                                  cAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCgt                                                                                                                  <  1:90685/149‑1 (MQ=255)
                                        gcGGTGACAGGATCGGGGTCGGCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGCATGTTGGCGTTGATACCACTGCGGCACAACTCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTaa                                                                                                             <  3:6647/147‑1 (MQ=255)
                                        ccGGTGACAGCATCGGTGTCGTGTTTGCTAATGTCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTTGCGGTGATACCACTGCGGCACAAATGCGCGTCGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCGTGCGTGTTaa                                                                                                             <  1:8515/148‑1 (MQ=255)
                                        ccGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAAt                                                                                                            <  8:397114/149‑1 (MQ=255)
                                                aGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAg                                                                                                                                <  3:118496/121‑1 (MQ=255)
                                                aGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAg                                                                                                                                >  4:118496/1‑121 (MQ=255)
                                                              cTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACGAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGc                                                                                                                    >  4:93843/1‑119 (MQ=255)
                                                              cTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACGAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGc                                                                                                                    <  3:93843/119‑1 (MQ=255)
                                                               tttGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACcgc                                                                                     >  2:12698/1‑149 (MQ=255)
                                                                 tGCTAATTGCGCATTTATGGATGTCGAGTAAACTCAGGGGAATGTTGGCTGGGATACCAGTGCGGCACAAATCCGCTACGCTCGACTTGACCCGAAAGTGAAAGATATTGTCCTGCGTGTTAATAGCCCAGGCGTCAGCGTTACcgc                                                                                     <  4:257534/147‑1 (MQ=255)
                                                                      aTGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTg                                                                                <  1:267363/147‑1 (MQ=255)
                                                                       tGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAg                                                                             <  4:121455/149‑1 (MQ=255)
                                                                           gcAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGaa                                                                              >  8:161189/1‑144 (MQ=255)
                                                                           gcAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGaa                                                                              <  7:161189/144‑1 (MQ=255)
                                                                               ttATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTcgc                                                                     <  1:12698/149‑1 (MQ=255)
                                                                               ttATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGAACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATGGTCCTGCGTGTGAATAGCCCAGGCGGCGGCGTTACCTCGTCTGAAGTGATTc                                                                       >  6:89673/1‑147 (MQ=255)
                                                                                           aGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTgg                                                           >  5:190596/1‑147 (MQ=255)
                                                                                             gAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGgcag                                                        >  4:323962/1‑148 (MQ=255)
                                                                                               aaCTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTTGcagcag                                                     >  7:197875/1‑149 (MQ=255)
                                                                                                aCTCAGGGGAATGTTGGCGGTGATACCACTCCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCagcagc                                                    >  6:135692/1‑149 (MQ=255)
                                                                                                  tCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGt                                                                                   <  2:215183/116‑1 (MQ=255)
                                                                                                  tCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGt                                                                                   >  1:215183/1‑116 (MQ=255)
                                                                                                  tCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGccc                                                  >  8:358966/1‑149 (MQ=255)
                                                                                                  tCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGccc                                                  >  4:324478/1‑149 (MQ=255)
                                                                                                  tCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGccc                                                  >  8:364344/1‑149 (MQ=255)
                                                                                                           tGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCgg                                         <  1:155927/149‑1 (MQ=255)
                                                                                                           tGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCgg                                         <  6:190596/149‑1 (MQ=255)
                                                                                                            gTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCg                                          <  4:88899/147‑1 (MQ=255)
                                                                                                            gTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCg                                          >  3:88899/1‑147 (MQ=255)
                                                                                                                          cACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTgg                                                           >  4:288505/1‑116 (MQ=255)
                                                                                                                          cACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTgg                                                           <  3:288505/116‑1 (MQ=255)
                                                                                                                          cACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTg                          >  4:232097/1‑149 (MQ=255)
                                                                                                                          cACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTg                          >  3:24351/1‑149 (MQ=255)
                                                                                                                            cTGCGGCACAAATCAGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTa                        >  5:284452/1‑149 (MQ=255)
                                                                                                                               cGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATc                      >  4:107371/1‑148 (MQ=255)
                                                                                                                                 gCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGAt                   >  6:153395/1‑149 (MQ=255)
                                                                                                                                   acaAATCCGCGACGCCCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGcc                                                                                                        >  7:87679/1‑62 (MQ=255)
                                                                                                                                   acaAATCCGCGACGCCCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGcc                                                                                                        <  8:87679/62‑1 (MQ=255)
                                                                                                                                     aaaTCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGggc               >  5:254733/1‑149 (MQ=255)
                                                                                                                                        tCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGACCTGGCATCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGggcgg             >  5:147503/1‑148 (MQ=255)
                                                                                                                                        tCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGggcgg             >  8:67366/1‑148 (MQ=255)
                                                                                                                                                  tCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTCCCGCGTCGGAAGTGATTCGCGCTGAACTGGCCGCAGCCCGGGCAGCGGGTTCGCCTGGGGTTGTATCGCTGggcggcatggcggcct  >  5:26305/1‑147 (MQ=255)
                                                                                                                                                  tCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGggcggcatggcggcat  >  5:325276/1‑149 (MQ=255)
                                                                                                                                                  tCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGggcggcatggcggcat  >  7:270552/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCAT  >  NC_000913/1849767‑1850061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑