Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,222 T→G 5.5% intergenic (+17/+35) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2220TG 5.5% 250.8 / 2.2 83intergenic (+17/+35)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (37/41);  new base G (2/3);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGA  >  NC_000913/2519077‑2519359
                                                                                                                                                 |                                                                                                                                         
gCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtatt                                                                                                                                        <  5:88330/149‑1 (MQ=255)
     tCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTAGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtctc                                                                                                                                   >  8:100625/1‑149 (MQ=255)
      cGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCAAGTGAAGGAATTAGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcc                                                                                                                                  >  8:398796/1‑149 (MQ=255)
       gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCCATTTTTGTCACGTGAAGGAATTCGCAATTTCCTAGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtattatagcct                                                                                                                                 >  5:73792/1‑144 (MQ=255)
       gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCt                                                                                                                                 >  2:87752/1‑149 (MQ=255)
                  aaCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATc                                                                                                                       <  4:206591/148‑1 (MQ=255)
                  aaCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                      >  4:89260/1‑149 (MQ=255)
                      cgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                                                  <  7:303561/149‑1 (MQ=255)
                       gatagataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                 <  7:13903/149‑1 (MQ=255)
                           gataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACg                                                                                                             >  4:193331/1‑149 (MQ=255)
                            ataataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                                                            >  3:282306/1‑149 (MQ=255)
                            ataataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                                                            >  8:118746/1‑149 (MQ=255)
                            ataataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                                                            >  1:337547/1‑149 (MQ=255)
                            ataataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGc                                                                                                            <  4:171568/149‑1 (MQ=255)
                            ataataTGACAGACAATGAGAAGGAAAAAGTCGAGCAATTATTGTCACGTGAAGGAATTCGCAATTTCATCGCCCGTCTCGATATTGACGAATCAGAATAAATAAAAAACGTCAGGATTTAATATCCTGCCGTTTATCTTTTTACACGc                                                                                                            >  6:187694/1‑149 (MQ=255)
                                tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAt                                                                                                        >  4:102132/1‑149 (MQ=255)
                                    agagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                                                  <  4:230526/133‑1 (MQ=255)
                                    acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAct                                                                                                    <  2:214098/149‑1 (MQ=255)
                                    acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                     <  1:179203/148‑1 (MQ=255)
                                    acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                     >  2:179203/1‑148 (MQ=255)
                                      agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtattat                                                                                                                                      >  5:262850/1‑113 (MQ=255)
                                      agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtattat                                                                                                                                      <  6:262850/113‑1 (MQ=255)
                                        acaTTGAGCAGGAAAAAGTCGCGGAATTTTTGTAAAGTGAAGGAATTCGCAATTTCCTCGACCGTCTCGATATTTAAGCATCAGCATAAATAAAAAAAGGCAGTATATTATCTCCTGCCGTTTATCTTTTTACACGATAATAActgct                                                                                                 >  1:310899/1‑148 (MQ=255)
                                          aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAt                                                                                              >  6:37048/1‑149 (MQ=255)
                                          aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAt                                                                                              >  3:58530/1‑149 (MQ=255)
                                             gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAttt                                                                                            <  7:369506/148‑1 (MQ=255)
                                               gCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                         >  2:318476/1‑149 (MQ=255)
                                                  ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                      >  2:310372/1‑149 (MQ=255)
                                                   gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                     <  4:112660/149‑1 (MQ=255)
                                                   gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCATGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                      >  1:26374/1‑148 (MQ=255)
                                                     aaaaGTCTCTCAATTTTTGTCACGTGAAGGAATTCTAAATTTCCTCGCCCGTCTCGATATTTACGAATCAGCATATATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                                                   <  8:320607/149‑1 (MQ=255)
                                                     aaaaGTCTCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                                                   <  1:125659/149‑1 (MQ=255)
                                                             cgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa                                                                           <  2:337547/149‑1 (MQ=255)
                                                              gcAATTTTTGTCACGTTAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                          >  2:154294/1‑149 (MQ=255)
                                                              gcAATTTTTGTCACGTTAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                          <  1:154294/149‑1 (MQ=255)
                                                              gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                          >  1:254248/1‑149 (MQ=255)
                                                              gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaaa                                                                          <  4:282306/149‑1 (MQ=255)
                                                                 aTTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGGCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATcc                                                                       <  6:201254/149‑1 (MQ=255)
                                                                    tttGTCACGTTAAGGAATTCGCAATTTCCTAGAACGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGTATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAg                                                                     <  1:319069/148‑1 (MQ=255)
                                                                    tttGTCACGTGAAGGAATTCGCATTTTCCTCGCCCGTCTCGTTATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCTATAAAATCCAg                                                                     <  8:289160/148‑1 (MQ=255)
                                                                       gTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACAAGCTAATTACTGCTGATTTTCGCGTTCAGCAATAACATCCAgcgat                                                                 >  2:329239/1‑147 (MQ=255)
                                                                          aCGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttg                                                              >  3:228565/1‑149 (MQ=255)
                                                                            gTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                            >  6:162268/1‑149 (MQ=255)
                                                                             tGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                            >  3:93579/1‑148 (MQ=255)
                                                                               atctaaTTCGCAATTTCCTCTCCCGTCTCGATATTGACGTATCAGAATAAATAAAATACGGCAGGATATTTTCTCATGCCGTTTATCTTTTTACACGATAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGa                                                          <  5:187694/144‑1 (MQ=255)
                                                                               aaGGAATTCTCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                         <  7:100625/149‑1 (MQ=255)
                                                                               aaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                         <  3:89260/149‑1 (MQ=255)
                                                                                     ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                   >  7:263219/1‑149 (MQ=255)
                                                                                          aaTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                            <  7:146036/135‑1 (MQ=255)
                                                                                               ccTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                          <  7:398796/148‑1 (MQ=255)
                                                                                               ccTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                          <  8:263219/148‑1 (MQ=255)
                                                                                                cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCgg                                         <  1:151139/148‑1 (MQ=255)
                                                                                                   gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                       <  4:58530/147‑1 (MQ=255)
                                                                                                   gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCt                                     <  2:26374/149‑1 (MQ=255)
                                                                                                     ccGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTa                                   <  1:310372/149‑1 (MQ=255)
                                                                                                      gaaaatcagcagtaattagcgtgtaaaaagatAAACGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc                                  >  5:88815‑M1/33‑149 (MQ=255)
                                                                                                      gaaaatcagcagtaattagcgtgtaaaaagatAAACGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATAAGCTCGATACTGCGGGTCTTAc                                  <  6:88815‑M1/117‑1 (MQ=255)
                                                                                                      cGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTAc                                  <  1:87752/149‑1 (MQ=255)
                                                                                                          tcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGGTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGAGGGTCTTACAAAt                              >  5:216460/1‑149 (MQ=255)
                                                                                                            gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAAtt                             <  5:37048/148‑1 (MQ=255)
                                                                                                            gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                            >  6:148391/1‑149 (MQ=255)
                                                                                                            gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                            <  7:118746/149‑1 (MQ=255)
                                                                                                             atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc                           <  3:102132/149‑1 (MQ=255)
                                                                                                                ttGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGTGTCTTACCAATTGCGt                         <  2:254248/148‑1 (MQ=255)
                                                                                                                ttGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGt                         <  1:318476/148‑1 (MQ=255)
                                                                                                                  gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGaa                      >  2:298234/1‑149 (MQ=255)
                                                                                                                    cGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                    <  4:93579/149‑1 (MQ=255)
                                                                                                                     gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACgg                   <  1:298234/149‑1 (MQ=255)
                                                                                                                     gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACgg                   >  8:319714/1‑149 (MQ=255)
                                                                                                                     gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACgg                   <  4:117496/149‑1 (MQ=255)
                                                                                                                     gAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTAATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCTATACTGCGGGTCTTACCAATTGCGTGAAct                    >  2:350650/1‑147 (MQ=255)
                                                                                                                          aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                          >  2:28796/1‑73 (MQ=255)
                                                                                                                          aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTc                                                                                          <  1:28796/73‑1 (MQ=255)
                                                                                                                          aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACa              >  5:286743/1‑149 (MQ=255)
                                                                                                                            cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc                           <  1:52198/134‑1 (MQ=255)
                                                                                                                            cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc                           >  2:52198/1‑134 (MQ=255)
                                                                                                                              taaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc            >  2:75000/1‑147 (MQ=255)
                                                                                                                               aaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGACTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAg         >  1:115958/1‑149 (MQ=255)
                                                                                                                                  taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc      >  5:139524/1‑149 (MQ=255)
                                                                                                                                  agatAAACGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc      <  5:366540‑M1/145‑1 (MQ=255)
                                                                                                                                    aaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg    <  6:55551/149‑1 (MQ=255)
                                                                                                                                      AAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCAAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACAAATTGc                           <  5:269077‑M1/124‑1 (MQ=255)
                                                                                                                                      AAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGACTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGga  >  2:115958‑M1/1‑149 (MQ=255)
                                                                                                                                      AAACGGCAGGAGATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg    <  1:104450‑M1/147‑1 (MQ=255)
                                                                                                                                       AACGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGga  >  6:366540‑M1/1‑148 (MQ=255)
                                                                                                                                             AGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg    <  6:202002‑M1/140‑1 (MQ=255)
                                                                                                                                             AGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg    >  5:202002‑M1/1‑140 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
GCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGA  >  NC_000913/2519077‑2519359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: