Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,126 C→A 7.6% intergenic (+25/+32) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1260CA 7.6% 248.0 / 7.0 79intergenic (+25/+32)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (38/35);  new base A (4/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

CAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGG  >  NC_000913/3044978‑3045271
                                                                                                                                                    |                                                                                                                                                 
cAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATAc                                                                                                                                                   >  3:356078/1‑149 (MQ=255)
 aaaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACt                                                                                                                                                  <  3:144878/149‑1 (MQ=255)
  aaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                 <  7:66499/149‑1 (MQ=255)
  aaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                 >  8:66499/1‑149 (MQ=255)
   aCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttg                                                                                                                                                <  8:62212/149‑1 (MQ=255)
    cGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGt                                                                                                                                               >  1:65918‑M2/1‑148 (MQ=255)
     gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              >  6:226758‑M2/1‑147 (MQ=255)
     gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              >  8:6544‑M2/1‑147 (MQ=255)
     gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              >  1:33669‑M2/1‑147 (MQ=255)
     gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              >  4:145062‑M2/1‑147 (MQ=255)
          ggCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTAttgcatc                                                                                                                                         >  1:6802‑M2/1‑142 (MQ=255)
            cTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccg                                                                                                                                       >  7:13453‑M2/1‑140 (MQ=255)
             tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccgg                                                                                                                                      <  8:139209‑M2/149‑11 (MQ=255)
              ttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccggt                                                                                                                                     <  6:120738‑M2/149‑12 (MQ=255)
               tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccgg                                                                                                                                      >  5:46366‑M2/1‑137 (MQ=255)
                aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccggt                                                                                                                                     <  5:381480‑M2/147‑12 (MQ=255)
                aTCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATAATTGTTGCATCCGGTCa                                                                                                                                   >  3:71001/1‑149 (MQ=255)
                aTCTATGTGAATAAACATGACGACGGTACTGGAGATATGACCCGATCACGTAACAAAACCTTATACTGGTACAAAGAGGTCATTGCCAGCTACGGCGAGTAGCTTAAAGTCCATCAAGTACCGCATGCAATACTTGTTGCATCCCGTCa                                                                                                                                   >  2:100016/1‑149 (MQ=255)
                     tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGa                                                                                                                                 <  7:102452/146‑1 (MQ=255)
                         aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTc                                                                                                                          >  7:333376/1‑149 (MQ=255)
                         aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTc                                                                                                                          <  6:355357/149‑1 (MQ=255)
                         aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTc                                                                                                                          >  7:200814/1‑149 (MQ=255)
                              aCATGCCGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGCTGATTGCAAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTcccgga                                                                                                                     >  5:322735/1‑146 (MQ=255)
                              aCATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGt                                                                                                                     >  8:332652/1‑149 (MQ=255)
                                aTGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGttt                                                                                                                   >  5:223478/1‑149 (MQ=255)
                                    cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAtt                                                                                                               <  4:363452/149‑1 (MQ=255)
                                    cgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATAATTGTTGCATCCGGTCAGACAACTTCCCCGTTTTAtt                                                                                                               <  4:71001/149‑1 (MQ=255)
                                     gacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                              >  2:75359/1‑149 (MQ=255)
                                     gacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAttt                                                                                                              >  8:259857/1‑149 (MQ=255)
                                      acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTc                                                                                                             <  1:234690/149‑1 (MQ=255)
                                                       tCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                 >  7:373057/1‑96 (MQ=255)
                                                       tCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACtt                                                                                                                                                 <  8:373057/96‑1 (MQ=255)
                                                       tCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                            <  7:139209/149‑1 (MQ=255)
                                                                 gTAAGAAGAGCTTTAACTGTTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTAACGGATGCAATACTTGTTGCATCCGGTCAGACAACTTACCTGtt                                                                                                                    <  6:155485/115‑1 (MQ=255)
                                                                 gTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTccc                                                                                  <  8:200814/149‑1 (MQ=255)
                                                                 gTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCATGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTccc                                                                                  <  2:232023/149‑1 (MQ=255)
                                                                    agaagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGt                                                                                >  8:330495/1‑148 (MQ=255)
                                                                       agagCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATACGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTACCGGAGACg                                                                            >  2:192798/1‑149 (MQ=255)
                                                                            tttAACTGGTACAAAGAGGTGATTTCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAATACAGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTaa                                                                         >  6:242464/1‑147 (MQ=255)
                                                                             ttAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAagag                                                                      >  2:260405/1‑149 (MQ=255)
                                                                                aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGCCAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCt                                                                   >  6:38781/1‑149 (MQ=255)
                                                                                     tACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTAt                                                               >  7:50775/1‑148 (MQ=255)
                                                                                      aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccggt                                                                                                                                     >  6:36844‑M2/1‑66 (MQ=255)
                                                                                      aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGttgcatccggt                                                                                                                                     <  5:36844‑M2/77‑12 (MQ=255)
                                                                                      aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACACCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCa                                                             >  6:202890/1‑149 (MQ=255)
                                                                                      aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCa                                                             >  1:46096/1‑149 (MQ=255)
                                                                                      aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCa                                                             >  1:52048/1‑149 (MQ=255)
                                                                                       cAAAGAGGTGATTGCCATCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCAc                                                            <  6:122944/149‑1 (MQ=255)
                                                                                        aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGGATTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                           >  2:308342/1‑149 (MQ=255)
                                                                                        aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTCGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                           >  1:38094/1‑149 (MQ=255)
                                                                                          agagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTta                                                         <  4:341264/149‑1 (MQ=255)
                                                                                            agGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                       <  2:397364/149‑1 (MQ=255)
                                                                                             ggTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagta                                                      <  7:332652/149‑1 (MQ=255)
                                                                                              gTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTAttgcatccggtacttcatcgacttaaagc                                                                                                                   <  2:21934‑M2/87‑30 (MQ=255)
                                                                                              gTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTAttgcatccggtacttcatcgacttaaagc                                                                                                                   >  1:21934‑M2/1‑58 (MQ=255)
                                                                                                 aTTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                   <  1:75359/148‑1 (MQ=255)
                                                                                                      cAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              <  6:14552‑M2/52‑3 (MQ=255)
                                                                                                      cAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGtt                                                                                                                                              >  5:14552‑M2/1‑50 (MQ=255)
                                                                                                      cAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACaa                                             <  5:120738/149‑1 (MQ=255)
                                                                                                        gCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATg                                           <  6:223478/149‑1 (MQ=255)
                                                                                                          aaCGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGc                                         <  3:86796/149‑1 (MQ=255)
                                                                                                                gagaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTgcgc                                   <  6:381480/149‑1 (MQ=255)
                                                                                                                   aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAc                                <  8:333376/149‑1 (MQ=255)
                                                                                                                         tAAGTCGATGAATTACCTGATGCAATACTTGTTGCATACGGTCAGACAACTTCCCTGTTTTATTTCCCGCACGCCAAATCGATATAAGTTCGCTTGACGTAAGAGGCTTTATCACTTAGTAGACAGACAATGGCCTTCGCGACCtctt                           <  6:322735/148‑1 (MQ=255)
                                                                                                                            gTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                       <  1:260405/149‑1 (MQ=255)
                                                                                                                            gTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGACAGACAACTTCCCTGTATTATTTCGCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGACGCTATATCACTTAGTAGCCACACAATGGCCTGCCCGACatcatatg                        >  4:30915/1‑143 (MQ=255)
                                                                                                                            gTCGATGAAGTACCGGATGCAATACTTGTCGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                       <  2:38094/149‑1 (MQ=255)
                                                                                                                             tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGcc                      >  5:305278/1‑149 (MQ=255)
                                                                                                                               gATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAagag                                                                      >  6:687/1‑99 (MQ=255)
                                                                                                                               gATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAagag                                                                      <  5:687/99‑1 (MQ=255)
                                                                                                                                aTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtctt                           <  2:50889/141‑1 (MQ=255)
                                                                                                                                aTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtctt                           >  1:50889/1‑141 (MQ=255)
                                                                                                                                aTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGt                   >  8:85791/1‑149 (MQ=255)
                                                                                                                                     tgaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccacc              >  4:77495/3‑149 (MQ=255)
                                                                                                                                     gTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccacc              <  4:356078/149‑1 (MQ=255)
                                                                                                                                       aCCGTATGCAATACTTGTTGCAGCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccacca             <  5:228759/148‑1 (MQ=255)
                                                                                                                                            aTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGc       <  3:304652/149‑1 (MQ=255)
                                                                                                                                              gCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGCCCTCTTCTGCCTGTCCACCACGCTGCAt     >  2:217914/1‑149 (MQ=255)
                                                                                                                                                aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgg   >  4:57551/1‑149 (MQ=255)
                                                                                                                                                 aTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACTATGGCCTGCGCGACCTCTTGTGCCTGTCCTCCACGCAGCATggg  >  7:365678/1‑149 (MQ=255)
                                                                                                                                                    cTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc          <  4:217745/138‑1 (MQ=255)
                                                                                                                                                    cTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc          >  3:217745/1‑138 (MQ=255)
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CAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGG  >  NC_000913/3044978‑3045271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: