Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,226,632	G→A	5.8%	V133V (GTG→GTA)	ygjI →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,226,632	0	G	A	5.8%	221.1 / 2.5	69	V133V (GTG→GTA)	ygjI	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (39/26);  new base A (2/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT  >  NC_000913/3226485‑3226779                                                                                                                                                    |                                                                                                                                                    ggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTg                                                                                                                                                     <  2:235081/148‑1 (MQ=255)  gCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCt                                                                                                                                                   <  5:237339/149‑1 (MQ=255)      ttattaCTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTc                                                                                                                                               >  5:203481/1‑149 (MQ=255)         ttaCTGCCTATACCTACTGGTTCGTAAACGTGTTCTTTTTCACCTCCCTGTTGACGCTCGTTAATGCTTATGCTTCGTATGCATTCCTCGCATACGATTATATTATGACGACGGATGCCACCACCATTATCAGTATGGTGCTGTTAGcc                                                                                                                                            >  3:327178/1‑149 (MQ=255)            cTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTtc                                                                                                                                         <  4:164164/149‑1 (MQ=255)             tGCCTATACCTACTGGTTCGTAAACCTGTTCTTATTCACCTCACTGTAGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATTCCGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCATtct                                                                                                                                        >  6:52077/1‑149 (MQ=255)                    aCCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTg                                                                                                                                 >  3:355004/1‑149 (MQ=255)                      gtACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTTTTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTgg                                                                                                                                <  6:99841/147‑1 (MQ=255)                       tACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGt                                                                                                                              >  5:144349/1‑149 (MQ=255)                            gTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTgg                                                                                                                                <  2:91933/142‑1 (MQ=255)                            gTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTgg                                                                                                                                >  1:91933/1‑142 (MQ=255)                            gTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTc                                                                                                                           <  4:355004/147‑1 (MQ=255)                            gTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTc                                                                                                                           <  3:216331/147‑1 (MQ=255)                                  aaaCCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACg                                                                                                                   >  2:335583/1‑149 (MQ=255)                                    aCCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACggg                                                                                                                 <  1:335583/149‑1 (MQ=255)                                     ccTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACgggg                                                                                                                >  2:304119/1‑149 (MQ=255)                                      cTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCACCGTGGGTTGCCCCCACCgggg                                                                                                                >  2:127922/1‑148 (MQ=255)                                        gTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACAAACGGGGCg                                                                                                              >  5:331202/1‑148 (MQ=255)                                           cTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGaaaa                                                                                                          >  4:91574/1‑149 (MQ=255)                                                 ccccTCACTGTTGCCGCGCTTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTg                                                                                                     <  4:264838/146‑1 (MQ=255)                                                  aCCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTACTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTggg                                                                                                   >  1:215915/1‑149 (MQ=255)                                                  aCCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTACTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTggg                                                                                                   >  3:319666/1‑149 (MQ=255)                                                    cTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGc                                                                                                 >  1:16701/1‑149 (MQ=255)                                                              ccGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTc                                                                                       >  4:304681/1‑149 (MQ=255)                                                                      tATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCAACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACtt                                                                               >  7:205338/1‑149 (MQ=255)                                                                       aTTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACtt                                                                               >  3:72682/1‑148 (MQ=255)                                                                       aTTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACtt                                                                               >  8:7754/1‑148 (MQ=255)                                                                          gCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCaa                                                                            <  6:203481/148‑1 (MQ=255)                                                                            ttATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTTTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGc                                                                         >  3:253741/1‑149 (MQ=255)                                                                              aTGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCt                                                                        >  4:51/1‑148 (MQ=255)                                                                               tGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTACTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGa                                                                      <  2:215915/149‑1 (MQ=255)                                                                               tGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTACTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGa                                                                      <  4:319666/149‑1 (MQ=255)                                                                                gCTTCGTATGCCTTCCTCTGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGTGGCGAAAATGTTGGGGCCAATTACCTCCGTCCCTTCAACGCTTAt                                                                     >  8:151259/1‑149 (MQ=255)                                                                                gCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCGCCACCTGGGTTTCCACAAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGAt                                                                     >  6:358861/1‑149 (MQ=255)                                                                                 cTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGa                                                                      >  2:290532/1‑147 (MQ=255)                                                                                 cTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGa                                                                      <  1:290532/147‑1 (MQ=255)                                                                                 cTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATAAGTATGGTGCTGTTAGCCTTCTCCACCTGGGTTTCCAACAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGAtg                                                                    >  2:174983/1‑149 (MQ=255)                                                                                  ttCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGAtgc                                                                   >  8:139316/1‑149 (MQ=255)                                                                                    cGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGAtgctg                                                                 <  1:304119/149‑1 (MQ=255)                                                                                        tGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGctgctg                                                              <  7:7754/148‑1 (MQ=255)                                                                                          ccTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGTGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGtt                                                            <  6:331202/148‑1 (MQ=255)                                                                                           cTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACAAACGGGGCGAAAATGTTGGGGCCAATTACATCCGTCACTACACCGCTGATGGTGCTGTTaa                                                          >  4:223186/1‑149 (MQ=255)                                                                                            ttCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAAc                                                         >  7:341624/1‑149 (MQ=255)                                                                                             tCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACg                                                        >  2:39751/1‑149 (MQ=255)                                                                                              ccTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACg                                                        <  4:72682/148‑1 (MQ=255)                                                                                                tCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGctc                                                     >  3:260124/1‑149 (MQ=255)                                                                                                tCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTG‑TAACGctct                                                    >  1:305559/1‑149 (MQ=255)                                                                                                  ggATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGctc                                                     <  3:91574/147‑1 (MQ=255)                                                                                                        gAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAt                                             >  1:392364/1‑149 (MQ=255)                                                                                                        gAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAt                                             >  6:203776/1‑149 (MQ=255)                                                                                                         aaTATATTATGACGCCGGTTGCCACCCCCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAATTGTTGGGGCCAATTACCTCCGTCACTTCCACGCTGATGCTGCTGTTAACGCTCTCCTGCagt                                            >  3:43466/1‑147 (MQ=255)                                                                                                                tatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTaa                                                          >  1:151678/1‑128 (MQ=255)                                                                                                                tatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTaa                                                          <  2:151678/128‑1 (MQ=255)                                                                                                                tatGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTgg                                     >  3:184746/1‑149 (MQ=255)                                                                                                                  tGACGCCGGTTGCCCCCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTgg                                     >  3:104130/1‑147 (MQ=255)                                                                                                                  tGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGc                                    <  6:144349/148‑1 (MQ=255)                                                                                                                   gACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAg                                  >  3:335948/1‑149 (MQ=255)                                                                                                                    aCGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAg                                  <  7:151259/148‑1 (MQ=255)                                                                                                                     cGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGt                                <  7:91956/149‑1 (MQ=255)                                                                                                                           gtGCCACCACCATTCTCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGGTTCCACCAACGGGGCGAAAATGTTGTGGCCTATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGgcg                          <  5:52077/148‑1 (MQ=255)                                                                                                                                accaccaTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGt                     >  6:276656/1‑149 (MQ=255)                                                                                                                                     caTTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTggcg                <  2:16701/149‑1 (MQ=255)                                                                                                                                          acactATTGTGCCGGTCGCCCTCCGCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTCGCGGCGTa           <  1:127922/145‑1 (MQ=255)                                                                                                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAt                                             <  6:352208/111‑1 (MQ=255)                                                                                                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACAt                                             >  5:352208/1‑111 (MQ=255)                                                                                                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTgg                      >  4:363165/1‑134 (MQ=255)                                                                                                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTgg                      <  3:363165/134‑1 (MQ=255)                                                                                                                                              tATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGc       <  4:260124/149‑1 (MQ=255)                                                                                                                                                  gTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctgc   <  5:203776/149‑1 (MQ=255)                                                                                                                                                   tGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCctgct  >  7:256220/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT  >  NC_000913/3226485‑3226779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑