| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 756,061:1 | +C | 39.2% | coding (155/1767 nt) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 756,061 | 1 | . | C | 39.2% | 30.5 / 213.6 | 97 | coding (155/1767 nt) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base . (28/31); new base C (21/17); total (49/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
CCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGA > NC_000913/755916‑756205 | ccAGTCAGAGAATTTGATGCAGTTGTGAGTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑Ca < 5:5087/148‑1 (MQ=255) cAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTAcc > 8:371785/1‑148 (MQ=255) gagaATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTAcc < 8:390174/146‑1 (MQ=255) gagaATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTAcc > 7:390174/1‑146 (MQ=255) gagaATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGt > 1:310830/1‑148 (MQ=255) gaATTTGATGCAGTTGTGATTGGGGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCTGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTg < 2:147366/148‑1 (MQ=255) tttGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTgcgc < 5:430297/148‑1 (MQ=255) ttGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCt > 6:112315/1‑148 (MQ=255) aTGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTg < 3:272019/147‑1 (MQ=255) cAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCTG‑CATTACCGTTGCGCTGGGTa < 5:112315/147‑1 (MQ=255) aGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAAt < 7:371785/148‑1 (MQ=255) ttGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATa > 1:167647/1‑148 (MQ=255) ttGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATAc > 3:227612/1‑148 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTgc < 4:407766/135‑1 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTgc > 3:407766/1‑135 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGt > 1:23807/1‑142 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGt < 2:23807/142‑1 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATAcc > 7:222530/1‑148 (MQ=255) tgtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGAAAATTTCCCAGAGCGGCCAGACATGTGCGCTGCTCTCTAAAGTCTTCCCGAGCCGTTCCCATACCGTTTCTGCGAAAGGCGG‑CATTACCGTTGCGCTGGGTAATAcc > 5:25114/1‑148 (MQ=255) gtgATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATAcc > 2:17166/1‑148 (MQ=255) aTTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCAt < 1:15345/147‑1 (MQ=255) ttGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGa < 2:93886/148‑1 (MQ=255) ttGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGGCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATg < 3:277467/148‑1 (MQ=255) tgggggCCGGTGGCGCAGGGTTGCGCGCGGAGCTGCCACTTTCCCAGCGCGGCCAGACCTGTGCGCTGCTCTATAAAGGCTTCCCGCCCCGGTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACGGTTGCGCTGGGTAATACCCCTGa < 1:57508/143‑1 (MQ=255) ggTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGa < 1:29294/147‑1 (MQ=255) tGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTg < 1:17166/148‑1 (MQ=255) cgcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGa < 2:151877/148‑1 (MQ=255) gcAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCTGCCATACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTTCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAAt < 6:25114/148‑1 (MQ=255) cAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATg > 3:91245/1‑148 (MQ=255) gTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa < 3:264023/137‑1 (MQ=255) gTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATaa > 4:264023/1‑137 (MQ=255) aTGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAggcgg‑c > 3:170235/1‑99 (MQ=255) aTGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAggcgg‑c < 4:170235/99‑1 (MQ=255) tGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCT‑AAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCata < 7:131119/147‑1 (MQ=255) gcgcgcGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATg > 7:365924/1‑148 (MQ=255) gcgcgGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTAACGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCatattt < 3:269419/147‑3 (MQ=255) cgcgGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGAGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACAGTTGCGCAGGGTAATACCCATGAAGATAACTGGGAATTGCATATGTa > 4:269419/1‑147 (MQ=255) gCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt > 6:291618/1‑148 (MQ=255) gCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt > 2:17212/1‑148 (MQ=255) gCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTG‑‑CTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGa > 2:297641/1‑147 (MQ=255) cAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTg > 7:208245/1‑147 (MQ=255) aaaTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGaa < 4:227612/148‑1 (MQ=255) aaaTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGaa < 8:222530/148‑1 (MQ=255) aaTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGaa < 6:454053/148‑1 (MQ=255) aTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTg < 7:92013/144‑1 (MQ=255) aTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTg > 8:92013/1‑144 (MQ=255) tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGCCCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATAAGTACGACACCGTGAAAgg > 1:436655/1‑148 (MQ=255) ttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTa > 6:74361/1‑133 (MQ=255) ttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTa < 5:74361/133‑1 (MQ=255) tCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAggg > 4:354461/1‑148 (MQ=255) tCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCTTATGTACGTCACCTTGAAAggg < 2:310830/147‑1 (MQ=255) gagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAgg > 4:149824/1‑142 (MQ=255) gagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAgg < 3:149824/142‑1 (MQ=255) agCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGAc < 5:297003/148‑1 (MQ=255) ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCGGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCACTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc > 3:296634/1‑148 (MQ=255) ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTatat > 1:334634/1‑148 (MQ=255) aGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGGGAAAGGGTCGGACTATATCgg > 3:149295/1‑148 (MQ=255) aGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGCACGACACCGTGAAAGGGTCGGACTATATCgg < 1:15221/148‑1 (MQ=255) gACCTGCGCGCTGCTCTCTAAAGTCTTCCCGACCCGGTCCCATACCGATTCTGCGCAAGGCGG‑CATTACCGTTGCTCTGGGTAATACCCATGAAGAAAACAGGGAATGGCATATGTACGACACCGTGAAAGGGGCGGACTATATCGGt < 5:312623/148‑1 (MQ=255) gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAc > 8:412787/1‑146 (MQ=255) tgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAcc < 1:287383/147‑1 (MQ=255) tgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCATGAAAGGGTCGGACTATATCGGTGACCAg < 4:91245/148‑1 (MQ=255) cgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg < 2:334634/147‑1 (MQ=255) cgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGa < 6:194802/148‑1 (MQ=255) ctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGa < 2:221710/96‑1 (MQ=255) ctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGa > 1:221710/1‑96 (MQ=255) ctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcg > 4:32359/1‑148 (MQ=255) gctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcgc > 3:251933/1‑148 (MQ=255) tctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAt > 7:9678/1‑148 (MQ=255) tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTg > 3:403538/1‑147 (MQ=255) tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa > 7:293441/1‑143 (MQ=255) tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa < 8:293441/143‑1 (MQ=255) aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGa > 4:386560/1‑132 (MQ=255) aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGa < 3:386560/132‑1 (MQ=255) aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGAGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATAACGGTGACCAGGACGCGATTGaa > 1:197718/1‑147 (MQ=255) aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGa < 8:208245/145‑1 (MQ=255) aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata > 3:152196/1‑148 (MQ=255) aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata > 8:100916/1‑148 (MQ=255) aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatat < 5:141649/148‑1 (MQ=255) aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatat < 8:365924/148‑1 (MQ=255) cTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatatat > 7:163133/1‑148 (MQ=255) cTTCCCGACCCGGTCCCATACCGGTTCCGCGCACGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTTCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtg < 4:250812/148‑1 (MQ=255) cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgtg > 3:104432/1‑148 (MQ=255) cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtg < 2:167647/146‑1 (MQ=255) cccGACCCGTTCCCATACAGGTTCTGCGCAAGGCGG‑CATTACCGGTGCGCGGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtg < 3:31212/145‑1 (MQ=255) ccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa < 1:369091/110‑1 (MQ=255) ccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa > 2:369091/1‑110 (MQ=255) aCCCGTGCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAAGACCCATGAAGATACCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGAGTATATCGGTGACCAGGCCGCGGTTGAATTTATGTGTaaa > 6:297194/1‑148 (MQ=255) cGTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAAcc > 4:436627/1‑148 (MQ=255) cGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg < 7:412787/148‑1 (MQ=255) gTTCCCATACCGTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg > 2:27963/1‑148 (MQ=255) ttCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCggg > 8:190859/1‑148 (MQ=255) tCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTATAACCGGGc > 8:406374/1‑148 (MQ=255) gTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc > 7:291197/1‑147 (MQ=255) gTTTCTGCGCAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc > 8:217357/1‑147 (MQ=255) cgcAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt < 3:436627/146‑1 (MQ=255) cgcAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt < 7:190859/145‑1 (MQ=255) cAAGGCGGCCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGaa > 6:36183/1‑148 (MQ=255) aaGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACt < 4:152196/148‑1 (MQ=255) gcgg‑cATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa < 8:9678/148‑1 (MQ=255) | CCAGTCAGAGAATTTGATGCAGTTGTGATTGGTGCCGGTGGCGCAGGTATGCGCGCGGCGCTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGG‑CATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGA > NC_000913/755916‑756205 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |