Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 4,504,598 IS5 (–) +4 bp 38.8% coding (541‑544/1218 nt) yjhB → putative MFS transporter, membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)48 (0.480) 42/256 NT 37.0% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4504601 79 (0.810)coding (544/1218 nt) yjhB putative MFS transporter, membrane protein
* ? NC_000913 = 3653230NA (NA)52 (0.510) 45/256 NT 38.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4504598 = 79 (0.810)coding (541/1218 nt) yjhB putative MFS transporter, membrane protein

GGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653085‑3653230
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTT  >  NC_000913/4504598‑4504744
                                                                                                                                                                                                                                                                                                     
GGACAAGAAAATCGCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTTAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCT                                                                                                                                                   <  3:117997/148‑1
              CTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGAT                                                                                                                                       >  6:51431/1‑146
                      GTCTGACGGCGCTTACTGCTGAATTCACTTTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAA                                                                                                                               <  5:640/146‑1
                      GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAA                                                                                                                                <  2:42485/145‑1
                      GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAA                                                                                                                                <  2:370175/145‑1
                        CTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGC                                                                                                                           >  1:120383/1‑148
                          GACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTC                                                                                                                         >  8:401089/1‑148
                          GACGGCGCTTACTGCAGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTC                                                                                                                         >  4:21157/1‑148
                           ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCT                                                                                                                          <  1:435391/146‑1
                                  TTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAG                                                                                                                  >  2:166869/1‑147
                                    ACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCTTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGT                                                                                                                 <  5:443128/146‑1
                                        CTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGAACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGG                                                                                                             <  2:172683/146‑1
                                        CTGAATTCACTGTCGGCGAAGGTAAGGTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGG                                                                                                             <  6:317694/146‑1
                                                ACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTG                                                                                                    <  1:375433/147‑1
                                                    TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGA                                                                                                <  5:347682/147‑1
                                                    TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGA                                                                                                >  7:232414/1‑147
                                                    TCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGAT                                                                                               <  4:63453/147‑1
                                                        CGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAA                                                                                            >  7:269814/1‑147
                                                                     ATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGTGACTTTTTCGAACCTTCACTAGTTCTTTGTATCAGAAAAAGTGCTACAGAAAGTCAGGAGTGGATTGAAGCTAAATATAAGTATAAAT                                                                               >  4:385669/1‑147
                                                                       GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTC                                                                                                                <  1:196657/112‑1
                                                                       GACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTC                                                                                                                >  2:196657/1‑112
                                                                         CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACA                                                                          >  1:219426/1‑148
                                                                           CATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAA                                                                                                                                >  5:229070/1‑92
                                                                           CATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAA                                                                                                                                <  6:229070/92‑1
                                                                             TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTT                                                                      <  3:373492/148‑1
                                                                              GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTT                                                                     >  7:250027/1‑148
                                                                              GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATAT                                                                                         <  7:294050/128‑1
                                                                              GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATAT                                                                                         >  8:294050/1‑128
                                                                               ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTG                                                                    >  6:187587/1‑148
                                                                                 GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTC                                                                  >  6:421274/1‑148
                                                                                 GAAACCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTTTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTGCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTC                                                                  <  1:388022/148‑1
                                                                                        GTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTT                                                            >  7:290960/1‑147
                                                                                         TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTC                                                           <  2:56820/147‑1
                                                                                         TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTT                                                            <  8:269814/146‑1
                                                                                         TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGG                                                                                                             >  4:254593/1‑97
                                                                                         TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGG                                                                                                             <  3:254593/97‑1
                                                                                             ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCAAGAAAGTCAGGAGTGGATGGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTGCAGAA                                                       >  4:176604/1‑147
                                                                                              TGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAA                                                     <  5:51431/148‑1
                                                                                                    CAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCAC                                                 <  7:401089/146‑1
                                                                                                      GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCT                                             >  2:140108/1‑148
                                                                                                      GATGACAAACATGATCTCATATCACGGACTTGTTCGCTCATTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTGAGGAGTGGTTTGAAGATAATTATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATgt                                             >  4:283861/1‑146
                                                                                                       ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATCGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTT                                            >  6:215370/1‑148
                                                                                                           CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAA                                        >  4:261701/1‑148
                                                                                                             AACATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATC                                      >  6:76553/1‑148
                                                                                                               CATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTC                                    >  3:442877/1‑148
                                                                                                                ATGATCTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTC                                    >  2:53930/1‑147
                                                                                                                     CTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAT                              >  3:60119/1‑148
                                                                                                                     CTCATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGGCAGGAGTGGATTGAAGATAAATCTAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGAT                              <  3:112847/148‑1
                                                                                                                        ATATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGA                               <  1:166869/144‑1
                                                                                                                         TATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTT                          <  2:120383/148‑1
                                                                                                                          ATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGA                                                                                                       >  3:165569/1‑70
                                                                                                                          ATCAGGGACTTGTTCGCACCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGA                                                                                                       <  4:165569/70‑1
                                                                                                                                            CCTTCCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTC       >  2:310968/1‑148
                                                                                                                                                CCCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTAT    <  5:187587/147‑1
                                                                                                                                                 CCTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATCGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTT  <  5:215370/148‑1
                                                                                                                                                                                                                                                                                                     
GGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3653085‑3653230
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAGTTCTTTGGATCAGAAAAAGTGCTCCAGAAAGTCAGGAGTGGATTGAAGATAAATATAAGGATAAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTT  >  NC_000913/4504598‑4504744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.