Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,260 C→T 5.8% intergenic (‑232/+198) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,2600CT 5.8% 341.5 / 6.0 122intergenic (‑232/+198)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base C (62/50);  new base T (4/3);  total (67/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 124 total aligned reads displayed.

ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCT  >  NC_000913/3392114‑3392407
                                                                                                                                                  |                                                                                                                                                   
aTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGc                                                                                                                                                     <  6:103735/147‑1 (MQ=37)
 tGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCg                                                                                                                                                   >  5:97668/1‑148 (MQ=37)
   ttCCGGTTTGAATGCCTGATACGACGCACATCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCgg                                                                                                                                                  <  6:256685/147‑1 (MQ=255)
     ccGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                                                                                               >  6:152191/1‑148 (MQ=34)
       ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                                                                                                             >  5:64726/1‑148 (MQ=32)
             tccgCCTGATAATACGCAAAGTTTCTCATCAGGCAACGTCTGTCGGATGCGGCTTAAACTCCTTATCAGACCTACTGTTCTGTTCCGTTTGTTGGACTGATAAGACTCACATCGTCTCTTTAGTAAACGGCTGCCGGATGCGGCGTaa                                                                                                                                       <  4:370835/145‑1 (MQ=2)
             tAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAGGGCTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTaa                                                                                                                                       <  2:69357/148‑1 (MQ=18)
             tAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGtga                                                                                                                                       <  6:64726/148‑3 (MQ=11)
              aGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTaa                                                                                                                                       <  8:384775/147‑1 (MQ=33)
                  cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc                                                                                                                                  >  2:413420/1‑148 (MQ=34)
                    gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTa                                                                                                                                        >  7:330992/1‑140 (MQ=33)
                    gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                                                                                                 >  4:358318/1‑147 (MQ=35)
                       aaGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                             >  6:103467/1‑148 (MQ=37)
                         gACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                           <  8:208542/148‑1 (MQ=37)
                           cGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                         >  8:460187/1‑148 (MQ=37)
                             cacaGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTCTAGGCCTGATAAGACTCACAGAGTCGCATCAGTCAACGGATGCCGGATGCGGCGTAAACGCCTTATCCGAca                                                                                                                        >  2:91154/1‑146 (MQ=11)
                                  cGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                  <  5:152191/148‑1 (MQ=37)
                                  cGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                  <  2:377205/148‑1 (MQ=37)
                                    tCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTa                                                                                                                <  4:385454/148‑1 (MQ=37)
                                      gCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                                                                                              <  8:376821/148‑1 (MQ=37)
                                         tCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTc                                                                                                           >  5:443638/1‑148 (MQ=37)
                                         tCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTc                                                                                                           >  7:155179/1‑148 (MQ=17)
                                              cacgggCTGTCGGAGGGGGCGTAAAGGCCGTATCCGCCCGACGGGTAGGTGCCGTGTGGAGGCCTGATATGACGCACAGCGTCGCATCTGGCAACGGCGGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                      <  8:149023/144‑1 (MQ=14)
                                                aCGGCTGTCGTATGCGGCGTAACGGCCTTATCCGACCTACGGTTTTGTGCAGTTTGTCGGCCTGATAAGACGCACAGCGTCTCATCATGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    <  6:219655/148‑1 (MQ=14)
                                                aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTTCCGGATGCTGCGTAAACGCATTATCCGACCTACTGTTATGTTCCGTATg                                                                                                     >  8:458559/1‑147 (MQ=14)
                                                aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    <  5:103467/148‑1 (MQ=255)
                                                aCGGCTGTCGGATGCGGCGTAAACGCCTTACCAGACCAACGTTTATGTTCCGTTTGTAGGCCTGATAAGACGGACATCGTCGCTTCAGGTAACGGCTGCCGGATGCGGCGTGAACGCCTTATCCTACCTACGGTTATGTTCCGTTTGt                                                                                                    <  4:362768/148‑1 (MQ=11)
                                                   gCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAgg                                                                                                 <  8:308143/148‑1 (MQ=255)
                                                       tCGGATGCGGCGCAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                             <  8:138569/148‑1 (MQ=255)
                                                        cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCTCATCAGGAAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                                                                             <  2:255783/147‑1 (MQ=255)
                                                        cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                            >  6:27292/1‑148 (MQ=255)
                                                          gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                                                                           >  6:401553/1‑147 (MQ=255)
                                                            tGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATAAGACGCACAGCGTATCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCAGACGTAAGGTTATGTTCAGTTTGTAGGCCTGTTag                                                                                         >  4:18759/1‑146 (MQ=14)
                                                                    aaaCGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGAAACGGCTGCCCGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCAGTTTGTAGGCCAAATAAGACGCACAg                                                                                >  1:289120/1‑148 (MQ=14)
                                                                        gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                            <  7:460187/148‑1 (MQ=255)
                                                                          cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGTGGCGTAAACGCCTTATCCGACATACGGATATGTTCCGTATCTATGCCTTATAAGACGCACAACGTcc                                                                           >  1:46992/1‑146 (MQ=14)
                                                                            tATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCCTCGCAt                                                                        >  2:276605/1‑148 (MQ=255)
                                                                              tCCGACCTACGTTTATTTTCGGTTTGTAGGCCTGATAAGACGCACAGATTCGAATCAGGCATCGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                       <  6:433334/147‑1 (MQ=11)
                                                                              tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCCTCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATACGACGCCCAGCGTCGcct                                                                        >  4:363315/1‑144 (MQ=255)
                                                                              tCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGt                                                                             <  5:387117/141‑1 (MQ=40)
                                                                               ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                     >  6:184507/1‑148 (MQ=39)
                                                                               ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCATGATAAGACGCACAGCGTc                                                                            >  6:387117/1‑141 (MQ=255)
                                                                               ccGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTATGTAGGCCGGTTAAGACGCACAGCGACGCATCAg                                                                     >  1:208006/1‑148 (MQ=255)
                                                                                       cGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGc                                                             >  3:393248/1‑148 (MQ=35)
                                                                                       cGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTCTAGGGCTGATAAGACGCAAAGCGACGCATCAGGCTACGGc                                                             >  1:78791/1‑148 (MQ=11)
                                                                                         gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGTGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    >  6:401240/1‑107 (MQ=255)
                                                                                         gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGTGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    <  5:401240/107‑1 (MQ=255)
                                                                                          ttATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGc                                                          >  2:204724/1‑148 (MQ=35)
                                                                                          ttATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGc                                                          >  3:123688/1‑148 (MQ=35)
                                                                                            aTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCg                                                        >  6:397852/1‑148 (MQ=35)
                                                                                              gTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGt                                                                                                        <  5:387945/98‑1 (MQ=39)
                                                                                               ttCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa                                                      <  3:358318/147‑1 (MQ=35)
                                                                                               ttCCCTTTGTAGGCCTGATAAGATGCACAGCGTCGCATCATGCAACGGCTGCCGGATGCGGCGTAAACGCCTTTTCCGACCTAGGGTTATGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa                                                      <  2:322271/147‑1 (MQ=11)
                                                                                                 ccGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                   >  3:218730/1‑148 (MQ=33)
                                                                                                  cGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcg                                                  >  3:109807/1‑148 (MQ=32)
                                                                                                   gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                 <  4:393248/148‑1 (MQ=32)
                                                                                                     ttGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGTAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                               >  2:461846/1‑148 (MQ=17)
                                                                                                        tAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGTCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGa                                            <  5:27292/148‑1 (MQ=18)
                                                                                                        tAGGCCTGATAAGACACACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTg                                             >  4:274848/1‑147 (MQ=17)
                                                                                                         tgtccTGATCAGTAGCACAGCGTCGAATCATGCAAGGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAGATACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGa                                            <  6:184725/144‑1 (MQ=11)
                                                                                                         aGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACACCGTCGCATCAGGCAACGGCTTCCGGATGCGGCGTGa                                            >  5:80815/1‑147 (MQ=18)
                                                                                                           gcatGATAAGACTCACAGCGTCGAATCAGGCAACGGCTGCCGTATGAGTTGTTAACTCCTTATCCGACCTACGTTTATGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACg                                         <  6:409984/145‑1 (MQ=11)
                                                                                                            ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGc                                        >  2:194338/1‑148 (MQ=34)
                                                                                                             cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGcc                                       >  1:35608/1‑148 (MQ=34)
                                                                                                              tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGa                                                      <  8:228557/132‑1 (MQ=32)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                          >  2:210713/1‑63 (MQ=35)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                          <  1:210713/63‑1 (MQ=35)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTGCCGGATGCGGGGTGAACGccc                                      >  5:230809/1‑147 (MQ=25)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt                                                     >  7:228557/1‑132 (MQ=32)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGccc                                      >  1:405721/1‑147 (MQ=35)
                                                                                                               gATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGccc                                      >  2:93064/1‑147 (MQ=35)
                                                                                                                aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCCACGGCTGCCGGATGCGGCGTGAACGCCCTa                                    >  3:403736/1‑148 (MQ=37)
                                                                                                                aTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTa                                    <  1:204724/148‑1 (MQ=37)
                                                                                                                 tAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTa                                    >  6:258614/1‑147 (MQ=17)
                                                                                                                  aaGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                                                                            <  7:258577/90‑1 (MQ=33)
                                                                                                                      cGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATACGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGAc                              >  7:244220/1‑148 (MQ=17)
                                                                                                                      cGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGt                                              >  3:332143/1‑132 (MQ=32)
                                                                                                                        cacaGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGAcc                             >  8:101956/1‑147 (MQ=17)
                                                                                                                          caGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTAc                          >  4:249342/1‑148 (MQ=37)
                                                                                                                          caGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTAc                          <  4:123688/148‑1 (MQ=37)
                                                                                                                           aGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                                                   >  8:306525/1‑90 (MQ=33)
                                                                                                                            gCGTCGCATCAGGCAACGGCTGCCGGATGTGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    <  1:275408/72‑1 (MQ=21)
                                                                                                                               tCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTa                     <  6:97668/148‑1 (MQ=17)
                                                                                                                                cGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                     >  4:270043/1‑99 (MQ=33)
                                                                                                                                cGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                     <  3:270043/99‑1 (MQ=33)
                                                                                                                                cGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTAt                    >  5:55000/1‑148 (MQ=37)
                                                                                                                                   aTCAGGCAACGGCTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGtt                 <  1:91154/148‑1 (MQ=21)
                                                                                                                                   aTCAGGCAACGGCGGCCGGATGCGGCGTAAACGCCTTATCCGACCTACTGTTATGTGCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGtt                 <  5:311350/148‑1 (MQ=255)
                                                                                                                                    tCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTc                >  7:3088/1‑148 (MQ=37)
                                                                                                                                    tCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTc                >  6:364980/1‑148 (MQ=37)
                                                                                                                                     cAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                                                                 <  2:286429/82‑1 (MQ=33)
                                                                                                                                        gCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGc            >  7:368317/1‑148 (MQ=40)
                                                                                                                                           aCGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGt                                                                                                    <  4:266078/57‑1 (MQ=35)
                                                                                                                                            cGGCTGTCGGATGCGGCTTAAACCCCTTATCCGACCTACGTTTATGTTCCGTTTGTAGGCCTGATTAGACTCACAGCTTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGt        <  6:443638/148‑1 (MQ=11)
                                                                                                                                            cGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGt        >  7:55195/1‑148 (MQ=255)
                                                                                                                                               cTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                      <  2:162063/83‑1 (MQ=33)
                                                                                                                                               cTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                      >  1:162063/1‑83 (MQ=33)
                                                                                                                                                 gCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                       <  2:353990/80‑1 (MQ=32)
                                                                                                                                                 gCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                       >  1:353990/1‑80 (MQ=32)
                                                                                                                                                  ccGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCt  >  3:37038/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTCTGGCTCGTTGGCCT  >  NC_000913/3392114‑3392407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: