Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,472,627 G→A 5.2% N296N (AAC→AAT fusA ← protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,472,6270GA 5.2% 385.5 / 6.4 116N296N (AAC→AATfusAprotein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base G (57/53);  new base A (3/3);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 117 total aligned reads displayed.

AGAGTTAACCACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGAT  >  NC_000913/3472480‑3472773
                                                                                                                                                   |                                                                                                                                                  
agagTTAACCACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTATGAACGCCAGTCCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCg                                                                                                                                                    >  8:136242/1‑148 (MQ=255)
 gagTTAACCACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGt                                                                                                                                                   <  1:5473/148‑1 (MQ=255)
    ttAACCACACCGGGGTAAACACGGAAGAATGGCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCTGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGa                                                                                                                                                <  4:89103/148‑1 (MQ=255)
        ccACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCg                                                                                                                                             >  3:277804/1‑147 (MQ=255)
        ccACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGc                                                                                                                                            <  8:238860/148‑1 (MQ=255)
         cacaCCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCa                                                                                                                                           <  4:295415/148‑1 (MQ=255)
          acacCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAg                                                                                                                                          >  6:34401/1‑148 (MQ=255)
                  gTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGt                                                                                                                                        >  1:175631/1‑142 (MQ=255)
                  gTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGt                                                                                                                                        <  2:175631/142‑1 (MQ=255)
                    aaaCACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAAc                                                                                                                                >  5:223141/1‑148 (MQ=255)
                      acacGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCg                                                                                                                              <  1:39960/148‑1 (MQ=255)
                        acGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCggg                                                                                                                            <  6:272720/148‑1 (MQ=255)
                         cGGAAGAAGGTCAGGTTACCAACAACCGGGTCGGGAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCgggg                                                                                                                           <  8:166109/148‑1 (MQ=255)
                         cGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCgggg                                                                                                                           <  4:344052/148‑1 (MQ=255)
                         cGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCgggg                                                                                                                           <  7:431039/148‑1 (MQ=255)
                         cGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTAGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGACTTTACCTTCGACCAGGATACAGTTGATCGCAGGTACGACAACCggg                                                                                                                            >  3:321511/1‑147 (MQ=255)
                          ggAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGa                                                                                                                          <  7:405578/148‑1 (MQ=255)
                           gaagaaGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGAt                                                                                                                         >  8:4896/1‑148 (MQ=255)
                             agaagGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGGCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATgg                                                                                                                       <  2:44606/148‑1 (MQ=255)
                              gaagGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGc                                                                                                                      <  5:215978/148‑1 (MQ=255)
                               aagGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATgg                                                                                                                       >  8:445645/1‑146 (MQ=255)
                                  gccAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACAGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAgg                                                                                                                   >  2:248343/3‑147 (MQ=255)
                                    cAGGTTACCAACAAACGGGTCGGTAGCGATTTTGACCGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGTGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTaa                                                                                                                <  2:160842/148‑1 (MQ=255)
                                     aGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAAt                                                                                                               <  2:385063/148‑1 (MQ=255)
                                       gTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACAGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCa                                                                                                             <  1:248343/148‑1 (MQ=255)
                                        ttACCCACAAACGGGTCGGTAGCGATTTTGCACGCCATTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCATTGATCGCAGGTACGTCAACCTGGGATGGCAGGTAATCaa                                                                                                            >  8:338664/1‑148 (MQ=255)
                                        ttACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCATTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCaa                                                                                                            >  8:338666/1‑148 (MQ=255)
                                        ttACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCATTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCaa                                                                                                            >  8:338667/1‑148 (MQ=255)
                                        ttACCAACAAACGGGGCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCaa                                                                                                            <  5:34401/148‑1 (MQ=255)
                                         tACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAAt                                                                                                           >  7:41347/1‑148 (MQ=255)
                                         tACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAAt                                                                                                           >  4:180596/1‑148 (MQ=255)
                                         tACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACAGTCGTCAAGGATACCGTTGATCGCAGGTCCGTCAACCGGGGAGGGCAGGTAATCAAt                                                                                                           >  4:287950/1‑148 (MQ=255)
                                           ccAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAAtt                                                                                                          <  1:286822/147‑1 (MQ=255)
                                           ccAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTa                                                                                                         <  3:180596/148‑1 (MQ=255)
                                            caacaaACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCGCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGGTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTAc                                                                                                        <  3:287950/148‑1 (MQ=255)
                                             aacaaACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTAcc                                                                                                       >  1:98546/1‑148 (MQ=255)
                                             aacaaACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTAcc                                                                                                       >  4:119070/1‑148 (MQ=255)
                                              acaaACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCg                                                                                                      >  4:51812/1‑148 (MQ=255)
                                                  aCGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATc                                                                                                  <  4:277804/148‑1 (MQ=255)
                                                           tAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         >  4:231715/1‑148 (MQ=255)
                                                           tAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         <  3:163698/148‑1 (MQ=255)
                                                           tAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCATTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         <  7:338666/148‑1 (MQ=255)
                                                           tAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACGTGCGTGACGTTGAGCCGGAGTGTCTTTACAGTCGTCCAGTAGACCATTGATCGCAGGTACCTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         <  7:338664/148‑1 (MQ=255)
                                                                  tttGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATg                                                                                                                        >  6:67733/1‑110 (MQ=255)
                                                                   ttGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAacac                                                                                 >  8:210792/1‑148 (MQ=255)
                                                                     gAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCt                                                                               >  7:76076/1‑148 (MQ=255)
                                                                      aaCGCCAGTGCAGAGAACGGCTCGTCCTCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTTATCAATTACCGCATCCAGCATCGCCTGAACACCtt                                                                              <  5:78758/148‑1 (MQ=255)
                                                                         gCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGt                                                                           <  6:395054/148‑1 (MQ=255)
                                                                          ccAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGtt                                                                          >  3:161538/1‑148 (MQ=255)
                                                                          ccAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGtt                                                                          >  2:465980/1‑148 (MQ=255)
                                                                            aGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCt                                                                        <  2:333673/148‑1 (MQ=255)
                                                                              tGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCtt                                                                       >  6:396118/1‑147 (MQ=255)
                                                                               gCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTg                                                                      >  3:409490/1‑147 (MQ=255)
                                                                               gCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTg                                                                      >  3:35925/1‑147 (MQ=255)
                                                                                cAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGGAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGa                                                                     <  7:136242/147‑1 (MQ=255)
                                                                                cAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGaa                                                                    >  6:185327/1‑148 (MQ=255)
                                                                                  gagaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACg                                                                  <  2:98546/148‑1 (MQ=255)
                                                                                    gaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACg                                                                  >  4:16566/1‑146 (MQ=255)
                                                                                    gaACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACg                                                                  >  6:394387/1‑146 (MQ=255)
                                                                                      aCGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCa                                                                                                    <  3:19625/110‑1 (MQ=255)
                                                                                      aCGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCa                                                                                                    >  4:19625/1‑110 (MQ=255)
                                                                                      aCGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGa                                                              <  1:113866/148‑1 (MQ=255)
                                                                                         gCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAAcc                                                           <  7:445645/148‑1 (MQ=255)
                                                                                          cTCGTCCTCACTTGCGTGACGTTCAGCCGGAGTGTCCTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACca                                                          <  6:191674/148‑1 (MQ=255)
                                                                                          cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAAt                                                                                                           >  1:272036/1‑99 (MQ=255)
                                                                                          cTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAAt                                                                                                           <  2:272036/99‑1 (MQ=255)
                                                                                             gTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACcaca                                                        <  5:396118/147‑1 (MQ=255)
                                                                                             gTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACcac                                                         <  7:4896/146‑1 (MQ=255)
                                                                                             gTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACcac                                                         <  8:308328/146‑1 (MQ=255)
                                                                                                atcaCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGCACGCAGAACCACAGGt                                                     >  5:317491/1‑147 (MQ=255)
                                                                                                   aCTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTAcc                                                 <  3:231715/148‑1 (MQ=255)
                                                                                                     ttGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAg                                               >  6:168270/1‑148 (MQ=255)
                                                                                                      tGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAg                                               >  1:368527/1‑147 (MQ=255)
                                                                                                            aCGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAgg                                                                                                                                         >  8:434051/1‑51 (MQ=255)
                                                                                                            aCGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAgg                                                                                                                                         <  7:434051/51‑1 (MQ=255)
                                                                                                            aCGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGgatgat                                         <  3:16566/147‑1 (MQ=255)
                                                                                                                tCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTc                                                                         <  7:34887/111‑1 (MQ=255)
                                                                                                                tCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTc                                                                         >  8:34887/1‑111 (MQ=255)
                                                                                                                 cAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         <  2:73436/94‑1 (MQ=255)
                                                                                                                 cAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGc                                                                                         >  1:73436/1‑94 (MQ=255)
                                                                                                                 cAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgt                                    <  5:394387/147‑1 (MQ=255)
                                                                                                                    ccGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGTAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCgttgtt                                >  7:149018/1‑148 (MQ=255)
                                                                                                                        aGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGa                            <  8:41347/148‑1 (MQ=255)
                                                                                                                         gtgtCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGaa                           <  5:168270/148‑1 (MQ=255)
                                                                                                                         gtgtCTTTACCGTCGTCCAGGAAACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATAGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGaa                           >  2:182346/1‑148 (MQ=255)
                                                                                                                             cTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAAcgcg                       >  5:160361/1‑148 (MQ=255)
                                                                                                                               ttACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCt                     >  8:60432/1‑148 (MQ=255)
                                                                                                                                 aCCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTg                    <  5:445440/147‑1 (MQ=255)
                                                                                                                                    gtcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACg                 >  6:456807/1‑147 (MQ=255)
                                                                                                                                    gtcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACg                 >  4:232217/1‑147 (MQ=255)
                                                                                                                                    gtcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACg                 >  2:21910/1‑147 (MQ=255)
                                                                                                                                    gtcgtcCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACg                 >  1:294464/1‑147 (MQ=255)
                                                                                                                                         ccAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCagag            >  7:289702/1‑147 (MQ=255)
                                                                                                                                            ggATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCAcc        >  1:89729/1‑148 (MQ=255)
                                                                                                                                              aTACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGAttt                                       >  7:145229/1‑115 (MQ=255)
                                                                                                                                              aTACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGAttt                                       <  8:145229/115‑1 (MQ=255)
                                                                                                                                              aTACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCtt      <  6:223141/148‑1 (MQ=255)
                                                                                                                                              aTACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCtt      >  4:349765/1‑148 (MQ=255)
                                                                                                                                                 ccGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGa   <  4:161538/148‑1 (MQ=255)
                                                                                                                                                  cGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTCCCGCATCCAGCATCGCCTGAACACCTTTGTTCGTGAACGCAGAACCACAGGTTACCAGGATGATTACGTACTTCAGAACGCGCTGCCGAATAGCACCTGTGAt  >  5:36127/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGAGTTAACCACACCGGAGTAAACACGGAAGAAGGTCAGGTTACCAACAAACGGGTCGGTAGCGATTTTGAACGCCAGTGCAGAGAACGGCTCGTCATCACTTGCGTGACGTTCAGCCGGAGTGTCTTTACCGTCGTCCAGGATACCGTTGATCGCAGGTACGTCAACCGGGGATGGCAGGTAATCAATTACCGCATCCAGCATCGCCTGAACACCTTTGTTCTTGAACGCAGAACCACAGGTTACCAGGATGATTTCGTTGTTCAGAACGCGCTGACGCAGAGCACCTTTGAT  >  NC_000913/3472480‑3472773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: