New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 249927628 (0.830)5 (0.160) 5/246 2.9 15.1% coding (606/1698 nt) ypdA sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
?NC_000913 = 2499293 30 (0.940)coding (623/1698 nt) ypdA sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Rejected: Frequency below/above cutoff threshold.

CGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTT  >  NC_000913/2499159‑2499438
                                                                                                                                       |                                                                                                                                                 
cgcgTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                                      <  4:86839/148‑1 (MQ=255)
cgcgTTGGGGATCGATATCGTCTCTAAAATCGGCATTCAAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                                      >  3:86839/1‑148 (MQ=255)
       gggATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCcaaca                                                                                                                               >  8:75003/1‑148 (MQ=255)
             gATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCAGCTTGGCCTGCCGCGCgcgctggcctctttttcgccctcaac                                                                                                                          <  8:124413‑M2/149‑27 (MQ=255)
                  cGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCAGCTTGGCCTGCCGCGCgcgctggcctctttttcgccctcaacgcttt                                                                                                                     >  7:124413‑M2/1‑118 (MQ=255)
                          aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCTGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGc                                                                                                           <  6:84664/149‑1 (MQ=255)
                          aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                                      >  6:32707/1‑122 (MQ=255)
                          aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATc                                                                                                                                      <  5:32707/122‑1 (MQ=255)
                          aaaTCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGc                                                                                                           >  6:67553/1‑149 (MQ=255)
                                   ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCAt                                                                                                        >  8:93758/1‑143 (MQ=255)
                                   ttCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCAt                                                                                                        <  7:93758/143‑1 (MQ=255)
                                       aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGt                                                                                                      >  1:118130/1‑141 (MQ=255)
                                       aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGt                                                                                                      <  2:118130/141‑1 (MQ=255)
                                       aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATa                                                                                                 <  2:104180/146‑1 (MQ=255)
                                       aaTGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATa                                                                                                 >  1:104180/1‑146 (MQ=255)
                                           aTCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTc                                                                                          <  5:67553/149‑1 (MQ=255)
                                             ccTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCAt                                                                                        >  7:67126/1‑149 (MQ=255)
                                                                       ttGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCcgc                                                              <  7:75003/149‑1 (MQ=255)
                                                                            cTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGc                                                                          >  3:110804/1‑132 (MQ=255)
                                                                            cTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGc                                                                          <  4:110804/132‑1 (MQ=255)
                                                                                   tGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGaaa                                                                      >  4:68993/1‑129 (MQ=255)
                                                                                   tGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGaaa                                                                      <  3:68993/129‑1 (MQ=255)
                                                                                                             gCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGGGGCGATTACTAATAc                        >  3:19307/1‑149 (MQ=255)
                                                                                                              ccAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggctcattggaatgccgattttagagacgatatcgatccccaacgcggtagtt                       <  1:179317‑M2/149‑125 (MQ=255)
                                                                                                               cAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttag                                                    >  1:128928‑M2/1‑24 (MQ=255)
                                                                                                               cAGCGCCGCGCGGCAGGCCAAGCTggcgctggcctctttttcgccctcaacgctttgcaccagaagcacaataaagccgatacagacgctaccgaggatcattggaatgccgattttag                                                    <  2:128928‑M2/119‑96 (MQ=255)
                                                                                                               cAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATAcc                       >  1:61212/1‑148 (MQ=255)
                                                                                                                    ccgcGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACAATCACGCCGATGCGGTGGCGATTACTAATACCGATCAt                 <  8:67126/149‑1 (MQ=255)
                                                                                                                           ggCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTg           <  1:92618/149‑1 (MQ=255)
                                                                                                                                gCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTa      >  1:164203/1‑149 (MQ=255)
                                                                                                                                  cAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCAt                 <  7:2636/136‑1 (MQ=255)
                                                                                                                                  cAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCAt                 >  8:2636/1‑136 (MQ=255)
                                                                                                                                  cAAGCTGGCGATGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATTCGGTTGCTATTACTAATACCTATCATGTGCTGGCCTATg    >  2:137894/1‑149 (MQ=255)
                                                                                                                                   aaGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGAt                                                           >  2:68215/1‑93 (MQ=255)
                                                                                                                                    aGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGtt  <  2:61212/149‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                 
CGCGTTGGGGATCGATATCGTCTCTAAAATCGGCATTCCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAAGAGGCCAGCGCCGCGCGGCAGGCCAAGCTGGCGCTGGATATCGCCAACAAAACGCTACCGCTGTTTCGCCATGTCAATAGCGAGTCATTACGCAAGGTCTGCGAAATTATCCGCGATGACATTCACGCCGATGCGGTGGCGATTACTAATACCGATCATGTGCTGGCCTATGTT  >  NC_000913/2499159‑2499438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.