New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 280538025 (0.760)4 (0.130) 4/240 3.5 13.1% coding (566/1203 nt) proV glycine betaine/proline ABC transporter periplasmic binding protein
?NC_000913 = 2805401 30 (0.980)coding (587/1203 nt) proV glycine betaine/proline ABC transporter periplasmic binding protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATT  >  NC_000913/2805236‑2805519
                                                                                                                                                |                                                                                                                                           
gCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACg                                                                                                                                           >  4:20298/1‑147 (MQ=255)
                        tGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGc                                                                                                                                       <  6:11500/127‑1 (MQ=255)
                        tGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGc                                                                                                                                       >  5:11500/1‑127 (MQ=255)
                               ggTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAAt                                                                                                               >  6:124108/1‑144 (MQ=255)
                               ggTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAAt                                                                                                               <  5:124108/144‑1 (MQ=255)
                                gTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCAc                                                                                                         >  1:76731/1‑149 (MQ=255)
                                                    ccACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGcc                                                                                                                                      <  4:3485/100‑1 (MQ=255)
                                                    ccACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGcc                                                                                                                                      >  3:3485/1‑100 (MQ=255)
                                                          cTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAAt                                                                               <  2:76731/149‑1 (MQ=255)
                                                             cccGGATGAACTCTCTGGCGGGATGCGTCAACGTGTTGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGt                                                                                    <  4:30775/141‑1 (MQ=255)
                                                             cccGGATGAACTCTCTGGCGCGATCCGTCAACGTGTGGGATTAGCCCGCCCGTTAGCGATTAATCCGGATATATTATTAAAGGACGAAGCCTACTCGGCGCTCGATCCATTAATTCGCACCTAGATGCAGGATGAGATGGt                                                                                    >  3:30775/1‑141 (MQ=255)
                                                              ccGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACa                                                                           <  3:20298/149‑1 (MQ=255)
                                                                      aCTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAg                                                                                               >  5:77287/1‑121 (MQ=255)
                                                                      aCTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAg                                                                                               <  6:77287/121‑1 (MQ=255)
                                                                        tctctGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGAt                                                                                                   >  8:99714/1‑115 (MQ=255)
                                                                        tctctGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGAt                                                                                                   <  7:99714/115‑1 (MQ=255)
                                                                                gggATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTAc                                                                            >  4:122715/1‑130 (MQ=255)
                                                                                gggATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTAc                                                                            <  3:122715/130‑1 (MQ=255)
                                                                                                        gCCCGCGCGTTAGCGATAAATCCGGATATATTATTAATGGACGAAGCCATCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGCAACATCCGCGCCCCATTCTCTTTATTTCCCACGATCt                                 >  8:20430/1‑149 (MQ=255)
                                                                                                                       aTTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAgg                                                                         >  3:38283/1‑94 (MQ=255)
                                                                                                                       aTTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAgg                                                                         <  4:38283/94‑1 (MQ=255)
                                                                                                                            tCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTg             >  4:100568/1‑149 (MQ=255)
                                                                                                                                      ttattaATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAAt   >  5:51229/1‑149 (MQ=255)
                                                                                                                                      ttattaATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAAt   >  1:23408/1‑149 (MQ=255)
                                                                                                                                        attaATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTTGCGACCGAAtt  <  6:51229/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
GCCGGGAAAAAGCCCTTGATGCACTGCGTCAGGTCGGGCTGGAAAATTATGCCCACAGCTACCCGGATGAACTCTCTGGCGGGATGCGTCAACGTGTGGGATTAGCCCGCGCGTTAGCGATTAATCCGGATATATTATTAATGGACGAAGCCTTCTCGGCGCTCGATCCATTAATTCGCACCGAGATGCAGGATGAGCTGGTAAAATTACAGGCGAAACATCAGCGCACCATTGTCTTTATTTCCCACGATCTTGATGAAGCCATGCGTATTGGCGACCGAATT  >  NC_000913/2805236‑2805519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.