New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 453014550 (1.510)6 (0.190) 4/246 3.5 11.2% coding (99/1314 nt) sgcC putative PTS system EIIC permease component
?NC_000913 = 4530173 47 (1.490)coding (71/1314 nt) sgcC putative PTS system EIIC permease component
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTT  >  NC_000913/4530012‑4530283
                                                                                                                                     |                                                                                                                                          
cAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCACATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAg                                                                                                                             >  1:8321/1‑149 (MQ=255)
cAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATAGCCATGACGATCACCAGTCCCAAACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAg                                                                                                                             >  2:96007/1‑149 (MQ=255)
  aCGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAggg                                                                                                                           <  5:123641/149‑1 (MQ=255)
       aaaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATg                                                                                                                       <  5:132232/148‑1 (MQ=255)
       aaaGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGc                                                                                                                      <  5:96467/149‑1 (MQ=255)
             aTGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCgg                                                                                                                    >  2:157752/1‑145 (MQ=255)
               gCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGAt                                                                                                               >  3:139452/1‑148 (MQ=255)
                 aGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                            <  6:91145/149‑1 (MQ=255)
                            gAAACGCTCAAACATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGa                                                                                                 >  3:1517/1‑149 (MQ=255)
                               aCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCa                                                                                              <  1:157752/149‑1 (MQ=255)
                                 gCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGCTCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCat                                                                                             >  5:151991/1‑148 (MQ=255)
                                     aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                            <  8:52519/129‑1 (MQ=255)
                                     aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAg                                                                                                            >  7:52519/1‑129 (MQ=255)
                                     aaTCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCatgat                                                                                          <  4:139452/147‑1 (MQ=255)
                                            aCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGAc                                                                                 >  5:6095/1‑149 (MQ=255)
                                                              aGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATa                                                                                      <  7:120850/126‑1 (MQ=255)
                                                              aGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATa                                                                                      >  8:120850/1‑126 (MQ=255)
                                                                  tGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGa                                                                                                                   <  4:60790/93‑1 (MQ=255)
                                                                  tGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGa                                                                                                                   >  3:60790/1‑93 (MQ=255)
                                                                       aTCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                      <  1:142061/101‑1 (MQ=255)
                                                                       aTCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAAt                                                                                                      >  2:142061/1‑101 (MQ=255)
                                                                        tCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATg                                                     <  4:1517/149‑1 (MQ=255)
                                                                           ccATGACGATCACCAGTCCCATAACGACAAAACCAATCACAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTaa                                                  >  8:22806/1‑149 (MQ=255)
                                                                                  gATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAAc                                            >  4:106618/1‑148 (MQ=255)
                                                                                      aCCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGTATGCGGAAGATAAGTACAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAAt                                       <  1:96007/149‑1 (MQ=255)
                                                                                        cAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGc                                     >  1:123639/1‑149 (MQ=255)
                                                                                                 aCCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTa                            >  3:90828/1‑149 (MQ=255)
                                                                                                  ccGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTa                            <  6:6095/148‑1 (MQ=255)
                                                                                                   agACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGTGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAACCGTTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAtt                          <  6:151991/148‑1 (MQ=255)
                                                                                                        aaaCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACTGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      >  5:81831/1‑148 (MQ=255)
                                                                                                        aaaCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      >  4:4732/1‑148 (MQ=255)
                                                                                                          aCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGa                        <  8:118514/144‑1 (MQ=255)
                                                                                                          aCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGa                        >  7:118514/1‑144 (MQ=255)
                                                                                                            cAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATaa                                        <  2:74746/126‑1 (MQ=255)
                                                                                                            cAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACa                 <  7:22806/149‑1 (MQ=255)
                                                                                                                   aaCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      >  2:68460/1‑137 (MQ=255)
                                                                                                                   aaCCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      <  1:68460/137‑1 (MQ=255)
                                                                                                                           ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACTGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt  <  6:81831/149‑1 (MQ=255)
                                                                                                                           ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt  >  6:49799/1‑149 (MQ=255)
                                                                                                                           ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt  <  4:90828/149‑1 (MQ=255)
                                                                                                                            cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCtt          >  7:46531/1‑140 (MQ=255)
                                                                                                                            cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCtt          <  8:46531/140‑1 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAAt                                                 <  4:1731/99‑1 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAAt                                                 >  3:1731/1‑99 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGtt                     <  2:160727/127‑1 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGtt                     >  1:160727/1‑127 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      <  1:8591/126‑1 (MQ=255)
                                                                                                                              cTGCCTTAATCGCCTGAAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                      >  2:8591/1‑126 (MQ=255)
                                                                                                                               tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACagag              <  6:3231/133‑1 (MQ=255)
                                                                                                                               tGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACagag              >  5:3231/1‑133 (MQ=255)
                                                                                                                                     |                                                                                                                                          
CAACGTCAAAGACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTT  >  NC_000913/4530012‑4530283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.