New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4133088 =97 (1.040)3 (0.030) 3/250 NT 3.2% coding (473/891 nt) metF 5,10‑methylenetetrahydrofolate reductase
?NC_000913 4133117 = 89 (1.010)coding (502/891 nt) metF 5,10‑methylenetetrahydrofolate reductase
Rejected: Frequency below/above cutoff threshold.

CGGAATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTTTGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4133242‑4133088
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttgcttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAAC  >  NC_000913/4133117‑4133263
                                                                                                                                                                                                                                                                                                              
CGGAATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTT                                                                                                                                                           <  8:41061/149‑1
 GGAATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTT                                                                                                                                                          >  8:283954/1‑149
  GAATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTTT                                                                                                                                                         <  3:378179/149‑1
   AATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGAAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTTTG                                                                                                                                                        <  7:265044/149‑1
      AATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTTTGCTT                                                                                                                                                     >  3:201370/1‑149
                                                                                                                        CAGATTAAGCAAATCCGCCTGAGCGCTTTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTG                                   >  4:280888/1‑149
                                                                                                                               AGCAAATCCGCCTGAGCGCTTTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAA                                                                                  <  3:249315/95‑1
                                                                                                                               AGCAAATCCGCCTGAGCGCTTTTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAA                                                                                  >  4:249315/1‑95
                                                                                                                                                    TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTAT       >  2:1321/1‑149
                                                                                                                                                       GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCT     >  4:9175/1‑148
                                                                                                                                                        CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAA   <  3:115106/149‑1
                                                                                                                                                        CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAA   >  7:148824/1‑149
                                                                                                                                                         TTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAAC  >  4:6552/1‑149
                                                                                                                                                                                                                                                                                                              
CGGAATAATTTCCACATCAATGCCCGCCGATACACAGCGGTCACGAAAACGCAGGTAGCTTTCGACATCGAAGAAGAACTGAGTAATCGCGCGGTTGGCTCCGGCATCCACTTTGCGTTTCAGATTAAGCAAATCCGCCTGAGCGCTTTTTGCTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4133242‑4133088
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttgcttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGCCGGTATCTAAC  >  NC_000913/4133117‑4133263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.