Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 670,147 C→T 5.3% A142A (GCG→GCA nadD ← nicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913670,1470CT 5.3% 244.7 / 2.4 76A142A (GCG→GCAnadDnicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (42/29);  new base T (2/2);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

ACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTC  >  NC_000913/670000‑670290
                                                                                                                                                   |                                                                                                                                               
aCCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGCTCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTgcg                                                                                                                                                >  3:324158/1‑149 (MQ=255)
aCCGTTATGCAAACGTTCGCGGATGATGGTCGCCTAGATGTTAAACCACGGCGTTTCAGCGAGATAAATTATACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAACCAGTTCTGGGATTGCGGTTgcg                                                                                                                                                >  8:353304/1‑149 (MQ=255)
   gTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCa                                                                                                                                             <  2:150723/149‑1 (MQ=255)
        gCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTc                                                                                                                                         >  6:256921/1‑148 (MQ=255)
          aaaCGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAg                                                                                                                                      >  8:101031/1‑149 (MQ=255)
          aaaCGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGGTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAg                                                                                                                                      >  6:12877/1‑149 (MQ=255)
           aaCGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATATGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGt                                                                                                                                     <  8:307736/149‑1 (MQ=255)
             cGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTgg                                                                                                                                   <  8:32503/149‑1 (MQ=255)
             cGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTgg                                                                                                                                   <  7:42874/149‑1 (MQ=255)
               ttCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTggg                                                                                                                                  >  1:354188/1‑148 (MQ=255)
                     gatgatGGTCGCCGAGATGTTAAACCACGGCGTTTGAGCCAGATAATTTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTg                                                                                                                           <  3:124818/149‑1 (MQ=255)
                     gatgatGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTg                                                                                                                           <  2:298653/149‑1 (MQ=255)
                              cGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAGGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGTGCCATTTCAAGTGGGTAACCTGGACGCCGa                                                                                                                   >  1:115375/1‑148 (MQ=255)
                              cGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGTGCCATTTCAAGTGGGTAACCTGGACGCCGa                                                                                                                   >  1:324475/1‑148 (MQ=255)
                               gCCGAGATGTTAAACCACGGCGGTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACa                                                                                                                 <  3:255169/149‑1 (MQ=255)
                                ccGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGa                                                                                                                   >  8:191012/1‑146 (MQ=255)
                                 cGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGa                                                                                                               >  7:179211/1‑149 (MQ=255)
                                    gaTGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGa                                                                                                            <  3:314359/149‑1 (MQ=255)
                                      tGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGAt                                                                                                           >  4:7435/1‑148 (MQ=255)
                                              cACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAAtgtg                                                                                                   >  7:9075/1‑148 (MQ=255)
                                                cGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCAt                                                                                                <  8:9075/149‑1 (MQ=255)
                                                 ggCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCAtt                                                                                               <  6:293475/149‑1 (MQ=255)
                                                    gTTTCAGCCATATAAATTTTACCGGCAGGATGAAGGTGAAGATCTTCCGGGTTATGTGTAAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTc                                                                                            <  4:324158/149‑1 (MQ=255)
                                                       tCAGCCAGATAAATTTTACCGGCAGGCTGAGGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGTGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  2:115375/148‑1 (MQ=255)
                                                       tCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGTGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  2:324475/148‑1 (MQ=255)
                                                       tCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  8:179211/148‑1 (MQ=255)
                                                       tCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  4:377252/148‑1 (MQ=255)
                                                         aGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCCTTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTa                                                                                       >  1:273292/1‑149 (MQ=255)
                                                            cAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCg                                                                                    >  8:199013/1‑149 (MQ=255)
                                                             aGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAGTGTGCATTGTCGAGTATCGt                                                                                   >  7:6923/1‑149 (MQ=255)
                                                              gATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAACTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGtt                                                                                  <  4:45766/149‑1 (MQ=255)
                                                               aTAAATTTTACCGGCAGGTTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGttt                                                                                 >  2:386376/1‑149 (MQ=255)
                                                               aTAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGttt                                                                                 >  1:145246/1‑149 (MQ=255)
                                                               aTAAATTTTACAGGCAGGCTGAATGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTAAAGCCATTGCTGGTATTGCGGTTGAGCAATTTCAAGTGGGTAACCTGTACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGttt                                                                                 >  2:98694/1‑149 (MQ=255)
                                                                           ggCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTAcc                                                                     <  7:191012/149‑1 (MQ=255)
                                                                           ggCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTAc                                                                      >  2:405842/1‑148 (MQ=255)
                                                                           ggCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGGTTCGTATTCGTAc                                                                      >  1:121214/1‑148 (MQ=255)
                                                                             cAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAg                                                                   <  4:178805/149‑1 (MQ=255)
                                                                              atgatGAAGGTGAAGATCTTCCGTGATATCTGTCAAATCACCTTCCAGCCATTGCTGGTATTGCGGTTCCGCCATATCAAGTGGGTAACCTGGACCCCGACAGACGATCAAATGTGCATTTTCTAGTATCGTTTCGTATTCGTACCAgg                                                                  <  7:328533/145‑1 (MQ=255)
                                                                                  tGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCg                                                               >  3:307389/1‑148 (MQ=255)
                                                                                      ggTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAg                                                          >  7:345431/1‑149 (MQ=255)
                                                                                      ggTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAg                                                          <  4:307389/149‑1 (MQ=255)
                                                                                       gTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAg                                                          >  8:327413/1‑148 (MQ=255)
                                                                                       gTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAg                                                          <  7:327413/148‑1 (MQ=255)
                                                                                        tGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  1:92984/115‑1 (MQ=255)
                                                                                        tGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          >  2:92984/1‑115 (MQ=255)
                                                                                         gAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtattc                                                                          <  5:86780/130‑1 (MQ=255)
                                                                                         gAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtattc                                                                          >  6:86780/1‑130 (MQ=255)
                                                                                         gAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGt                                                        >  7:91008/1‑148 (MQ=255)
                                                                                                 tCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCAGAAAGGTCAGCAGTg                                                >  4:360047/1‑148 (MQ=255)
                                                                                                         aTGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          <  8:244527/98‑1 (MQ=255)
                                                                                                         aTGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGa                                                                                          >  7:244527/1‑98 (MQ=255)
                                                                                                          tgtgTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGAc                                       <  5:256921/148‑1 (MQ=255)
                                                                                                          tgtgTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGAc                                       <  6:100743/148‑1 (MQ=255)
                                                                                                          tgtgTCAAATGATCTTCCAGCCATTGCTAGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGAc                                       >  8:49319/1‑148 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGt                                                                                                                                 <  1:59919/55‑1 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGt                                                                                                                                 >  2:59919/1‑55 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaa                                    >  6:146762/1‑148 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaa                                    >  2:54887/1‑148 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGGATTCGTACCAGGTCGGAAAGGACAGCAGTGAATCCGGACCaa                                    >  6:64354/1‑148 (MQ=255)
                                                                                                             gtCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTACGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaa                                    >  1:96985/1‑148 (MQ=255)
                                                                                                                aaaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaataa                                 >  2:321591/1‑148 (MQ=255)
                                                                                                                aaaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaataa                                 >  6:179516/1‑148 (MQ=255)
                                                                                                                 aaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGAc                                       >  6:15847/1‑141 (MQ=255)
                                                                                                                 aaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGAc                                       <  5:15847/141‑1 (MQ=255)
                                                                                                                  aTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAataata                               >  6:99924/1‑148 (MQ=255)
                                                                                                                        ttCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGc                          >  5:35770/1‑147 (MQ=255)
                                                                                                                        ttCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGc                          >  4:257368/1‑147 (MQ=255)
                                                                                                                          ccAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGCCCAATAATAAACGCCAGc                      >  4:192604/1‑149 (MQ=255)
                                                                                                                          ccAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAg                       <  1:386376/148‑1 (MQ=255)
                                                                                                                           cAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAACCGACAGCg                     >  5:30295/1‑149 (MQ=255)
                                                                                                                           cAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACAAGGTCGGAAAGTTCAGCAGTGAATCCTGACCAATAATAAACCCCATCg                     >  5:24327/1‑149 (MQ=255)
                                                                                                                                aTTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACg                >  3:127960/1‑149 (MQ=255)
                                                                                                                                          ttGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCtt      >  2:385223/1‑149 (MQ=255)
                                                                                                                                            gCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGTATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCGTTGt    >  6:147042/1‑149 (MQ=255)
                                                                                                                                            gCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGt    >  7:256607/1‑149 (MQ=255)
                                                                                                                                             cGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGt    <  5:12877/148‑1 (MQ=255)
                                                                                                                                              ggTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTc  <  1:385223/149‑1 (MQ=255)
                                                                                                                                                   cgcCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTAcc                                                                     <  6:389266/77‑1 (MQ=255)
                                                                                                                                                   cgcCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTAcc                                                                     >  5:389266/1‑77 (MQ=255)
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ACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTC  >  NC_000913/670000‑670290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: