| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 756,966 | C→T | 8.8% | H354Y (CAC→TAC) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 756,966 | 0 | C | T | 8.8% | 233.9 / 9.9 | 79 | H354Y (CAC→TAC) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base C (37/35); new base T (3/4); total (40/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01 | |||||||||||
GTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGG > NC_000913/756820‑757111 | gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGCTGAAAGAGCCGATTCCGG‑TATCCCAACCTGTCAc < 6:71825/148‑1 (MQ=255) tCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGTAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc > 7:167434/1‑148 (MQ=255) ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTa > 1:9022/1‑149 (MQ=255) ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGGCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgatg < 7:83600/149‑1 (MQ=255) ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATggg > 5:89465/1‑149 (MQ=255) ccACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATggg > 5:3116/1‑149 (MQ=255) acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGCGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg > 5:72802/1‑149 (MQ=255) cgcgAAACTGAAACTCGAGCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTa < 8:200428/149‑1 (MQ=255) cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTAtt > 2:406298/1‑149 (MQ=255) gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc > 2:302856/1‑149 (MQ=255) ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc < 8:238520/149‑1 (MQ=255) tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa > 8:37096/1‑146 (MQ=255) tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa < 7:37096/146‑1 (MQ=255) gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc > 2:416750/1‑149 (MQ=255) gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc < 2:248307/149‑1 (MQ=255) tCACCTGGGTAAAGAAGTTCTCTAATCCCGTCTGCCTGGTATCCTGGAGCTTTCGCTTACCTTCGCTCACGTGGTTCCGTTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACTTGATGGGCGGTATTCCGACCAAAGTTACCg < 3:96289/149‑1 (MQ=255) ccTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGt > 7:381459/1‑148 (MQ=255) ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg < 4:356657/148‑1 (MQ=255) ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTATTCCGACCAAAGTTACCGGTCAGGc < 1:406298/149‑1 (MQ=255) ggTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGACGATTCCGGTTATCCCAACCTGTCACTACATGATCGGCGGTATTCCGACCAAAGTTACCGGTCAgg > 4:168544/1‑148 (MQ=255) aaaGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCact > 2:242280/1‑149 (MQ=255) gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac > 8:339310/1‑149 (MQ=255) gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac > 5:315101/1‑149 (MQ=255) gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt < 4:369407/149‑1 (MQ=255) gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt < 1:302856/149‑1 (MQ=255) ttCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt > 7:329922/1‑148 (MQ=255) ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa < 6:3116/148‑1 (MQ=255) ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGGCAGGCACTGACTGTGa < 6:89465/148‑1 (MQ=255) gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAAt < 8:381459/147‑1 (MQ=255) tCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga > 8:306534/1‑148 (MQ=255) ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa < 6:315101/148‑1 (MQ=255) ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa < 7:306534/148‑1 (MQ=255) ccGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAg < 3:384134/149‑1 (MQ=255) tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa > 6:409563/1‑137 (MQ=255) tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa < 5:409563/137‑1 (MQ=255) ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATTATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg > 6:130438/1‑145 (MQ=255) ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg > 6:399291/1‑145 (MQ=255) ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg > 8:375686/1‑145 (MQ=255) ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCACAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg > 8:29375/1‑145 (MQ=255) tATCCTGGAGCTTTCCCGTCCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg < 2:289337/149‑1 (MQ=255) tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg < 4:82851/149‑1 (MQ=255) tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg < 1:242280/149‑1 (MQ=255) tCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgt < 7:339310/148‑1 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAt > 1:154273/1‑140 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGAt < 2:154273/140‑1 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt > 6:123159/1‑149 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt > 4:31095/1‑149 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt > 4:24517/1‑149 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt > 2:228791/1‑149 (MQ=255) tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGgttgtt > 2:222101/1‑149 (MQ=255) gAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTcc > 7:347658/1‑149 (MQ=255) cTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCgg > 6:90161/1‑148 (MQ=255) tttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGa > 7:284182/1‑149 (MQ=255) ttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGAc > 5:181915/1‑149 (MQ=255) ccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg > 1:158536/1‑134 (MQ=255) ccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTgg < 2:158536/134‑1 (MQ=255) ccGTACCTTCGCGCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGTGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGt < 8:150842/149‑1 (MQ=255) gTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCCACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGttt < 2:96520/149‑1 (MQ=255) ttCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTTCCGt < 1:228791/149‑1 (MQ=255) cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGt < 1:222101/147‑1 (MQ=255) cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGt < 3:31095/147‑1 (MQ=255) gCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg < 5:130438/149‑1 (MQ=255) gCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTg < 1:171418/148‑1 (MQ=255) aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg < 1:214320/145‑1 (MQ=255) aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTgg > 2:214320/1‑145 (MQ=255) gTCGCTCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAAt > 6:119094/1‑149 (MQ=255) cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCTGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgt < 8:347658/149‑1 (MQ=255) ggTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg > 4:16107/1‑149 (MQ=255) gagCCGATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGt < 5:90161/149‑1 (MQ=255) gCCGATTCCGGTTATCACAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTac < 7:29375/149‑1 (MQ=255) gATTCCGGTTATCCCAACCTGTTACTACATGATGTGCGGTATTCCGACCAAAGTTACCGTTCAGGCAATGACTGAGAATGAGAAAGGCGAAGATGTGGTTGTTACGGGACTGTTTCCCGATGCTGaaa > 8:76950/1‑128 (MQ=255) gATTCCGGTTATCCCAACCTGTTACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGaaa < 7:76950/128‑1 (MQ=255) aTTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACgg > 3:378569/1‑149 (MQ=255) tCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGt > 7:183791/1‑149 (MQ=255) ccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTc < 3:16107/148‑1 (MQ=255) aaCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTgg < 8:284182/149‑1 (MQ=255) cTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcg > 4:345642/1‑149 (MQ=255) gTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcgg > 6:11374/1‑148 (MQ=255) tCACTACATGATGGGCGGTTTTCCGACCAAAGTAACCGGTCAGGCACTGACTGTGAATGAGAAAGGATTTGATGTTGTTGTTCCGGGACTGTTTGCCTTTGGTTAAATCTCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcgg < 6:66441/147‑1 (MQ=255) | GTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGG > NC_000913/756820‑757111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |