Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,713 G→A 5.1% intergenic (‑138/+153) artJ ← / ← artM arginine ABC transporter periplasmic binding protein/arginine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,7130GA 5.1% 324.6 / 3.0 100intergenic (‑138/+153)artJ/artMarginine ABC transporter periplasmic binding protein/arginine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (48/46);  new base A (2/3);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 106 total aligned reads displayed.

AACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGA  >  NC_000913/900565‑900850
                                                                                                                                                    |                                                                                                                                         
aactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                                                                                           >  7:141382/1‑149 (MQ=255)
 actTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                                                                                           <  8:3513/148‑1 (MQ=255)
   tttttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGtt                                                                                                                                        >  7:26095/1‑149 (MQ=255)
   tttttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGtt                                                                                                                                        >  8:15874/1‑149 (MQ=255)
      ttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGt                                                                                                                                      <  2:247517/148‑1 (MQ=255)
         atgatgGAACGCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCg                                                                                                                                   <  6:252672/148‑1 (MQ=255)
          tgatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTc                                                                                                                                 <  7:81833/149‑1 (MQ=255)
           gatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCt                                                                                                                                >  3:127333/1‑149 (MQ=255)
           gatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCt                                                                                                                                >  6:221265/1‑149 (MQ=255)
            atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCt                                                                                                                                <  5:329148/148‑1 (MQ=255)
            atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTAAATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCtt                                                                                                                               <  5:210355/149‑1 (MQ=255)
              ggAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCg                                                                                                                                   <  1:95135/143‑1 (MQ=255)
              ggAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCg                                                                                                                                   >  2:95135/1‑143 (MQ=255)
                aaCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTggg                                                                                                                                          >  8:107732/1‑134 (MQ=255)
                aaCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTggg                                                                                                                                          <  7:107732/134‑1 (MQ=255)
                 aCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATta                                                                                                                          <  4:43801/149‑1 (MQ=255)
                 aCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATta                                                                                                                          <  8:26095/149‑1 (MQ=255)
                  cTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTTCGGCATGTGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATtat                                                                                                                         <  7:410111/149‑1 (MQ=255)
                   tCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATtat                                                                                                                         >  4:117528/1‑148 (MQ=255)
                     ccGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATaa                                                                                                                       <  3:162550/148‑1 (MQ=255)
                     ccGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATaa                                                                                                                       <  3:117528/148‑1 (MQ=255)
                       gTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGaa                                                                                                                    <  4:363710/149‑1 (MQ=255)
                        tcagTCAATCTTATGATTTTTTGCCGTGTCTGCGGCATTGTATAACAATTTCATCAACTGATTTTATATGAAATAAACATTTTTAAATAATTTTAATGAAATAAATCAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAt                                                                                                                   <  2:351270/146‑1 (MQ=255)
                        tCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAAt                                                                                                                   <  4:114877/149‑1 (MQ=255)
                           gTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTa                                                                                                                >  1:64028/1‑149 (MQ=255)
                            tCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTAt                                                                                                               >  7:98637/1‑149 (MQ=255)
                              aaTCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATaa                                                                                                                       >  5:93572/1‑139 (MQ=255)
                              aaTCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATaa                                                                                                                       <  6:93572/139‑1 (MQ=255)
                              aaTCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATg                                                                                                              <  7:259614/148‑1 (MQ=255)
                              aaTCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATg                                                                                                              <  5:417011/148‑1 (MQ=255)
                                    aTGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACt                                                                                                          <  8:105088/146‑1 (MQ=255)
                                    aTGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATg                                                                                                       >  2:358957/1‑149 (MQ=255)
                                     tGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                                                                                           <  7:102833/112‑1 (MQ=255)
                                     tGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATGTATATGCAATAAACATGAATAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATg                                                                                                       >  6:363419/1‑148 (MQ=255)
                                       aTTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTc                                                                                                                                     >  6:101295/1‑116 (MQ=255)
                                       aTTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTa                                                                                                     <  8:239860/148‑1 (MQ=255)
                                        tttttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAgg                                                                                                   <  3:358651/149‑1 (MQ=255)
                                               ccGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGAt                                                                                             >  7:30529/1‑148 (MQ=255)
                                                cGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATaa                                                                                           <  6:80711/149‑1 (MQ=255)
                                                 gtgtCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAg                                                                                          <  4:127333/149‑1 (MQ=255)
                                                 gtgtCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAg                                                                                          <  2:64028/149‑1 (MQ=255)
                                                      tGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTc                                                                                     >  8:237929/1‑149 (MQ=255)
                                                      tGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTc                                                                                     >  3:94494/1‑149 (MQ=255)
                                                           cATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCTTTTTtattt                                                                                                                       <  5:36876/110‑3 (MQ=25)
                                                           cATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCTTTTTtattt                                                                                                                       >  6:36876/1‑108 (MQ=25)
                                                              gggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCccgccg                                                                              >  4:222305/1‑148 (MQ=255)
                                                              gggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCttt                                                                                                                              >  2:97565/1‑99 (MQ=38)
                                                              gggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCttt                                                                                                                              <  1:97565/100‑2 (MQ=38)
                                                                gATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAt                                                                           >  6:408030/1‑149 (MQ=255)
                                                                gATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGAGAAGGCGTCCCCGCCGCAt                                                                           >  5:385800/1‑149 (MQ=255)
                                                                     cAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCgg                                                                       >  3:348500/1‑148 (MQ=255)
                                                                          ccATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAgg                                                                 >  4:195179/1‑149 (MQ=255)
                                                                           cATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAgg                                                                 >  2:287484/1‑148 (MQ=255)
                                                                              cAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCAcc                                                             >  8:163288/1‑149 (MQ=255)
                                                                               aaGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCAcc                                                             <  4:348500/148‑1 (MQ=255)
                                                                                  tGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGc                                                         <  8:30529/149‑1 (MQ=255)
                                                                                   gAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGc                                                         >  5:165994/1‑148 (MQ=255)
                                                                                   gAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGc                                                         <  6:165994/148‑1 (MQ=255)
                                                                                   gAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCt                                                        >  3:213223/1‑149 (MQ=255)
                                                                                     aTTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGa                                                      >  2:77141/1‑149 (MQ=255)
                                                                                     aTTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCAGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGa                                                      >  6:394598/1‑149 (MQ=255)
                                                                                      tttATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGAt                                                     >  6:72319/1‑149 (MQ=255)
                                                                                       ttATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGTACAACTTAGTGGGTTGTCCGACTTCATTATAAGAATTTATACACTATGTAGGCCGGATAAGGCGTCACCGCCGCATACTGCACAGGCACCGTGCTGATg                                                    >  1:62411/1‑149 (MQ=255)
                                                                                       ttATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGACTTCATTATAAGAATTTATGCACTATGAAGGCCGCATAAGGCGTCACCGCCGCATACGGCACAGGCACCCTGCTGATg                                                    >  1:68575/1‑149 (MQ=255)
                                                                                         atatGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGt                                                   <  2:111675/148‑1 (MQ=255)
                                                                                          tatGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTc                                                  <  4:94494/148‑1 (MQ=255)
                                                                                            tGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCgg                                                                       <  2:339390/125‑1 (MQ=255)
                                                                                            tGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCgg                                                                       >  1:339390/1‑125 (MQ=255)
                                                                                            tGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGa                                               >  1:279284/1‑149 (MQ=255)
                                                                                            tGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGa                                               <  6:380355/149‑1 (MQ=255)
                                                                                               aataatCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAACCGGCACAGGCACCGTGCTGATGTCTGATGc                                            >  8:197905/1‑149 (MQ=255)
                                                                                               aaTAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGc                                            >  7:275283/1‑149 (MQ=255)
                                                                                               aaTAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGc                                            >  1:238648/1‑149 (MQ=255)
                                                                                                 tAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGa                                          <  3:222305/149‑1 (MQ=255)
                                                                                                    aCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGc                                       >  7:387269/1‑149 (MQ=255)
                                                                                                    aCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTGAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGc                                       >  5:268470/1‑149 (MQ=255)
                                                                                                     cATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCt                                      <  7:163288/149‑1 (MQ=255)
                                                                                                        gATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGgc                                   >  7:390649/1‑149 (MQ=255)
                                                                                                         aTTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGgcg                                  >  2:157291/1‑149 (MQ=255)
                                                                                                         aTTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGgc                                   <  1:392914/148‑1 (MQ=255)
                                                                                                             aataatTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATACGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTc                              <  5:221265/149‑1 (MQ=255)
                                                                                                             aataatTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTc                              >  6:206368/1‑149 (MQ=255)
                                                                                                             aataatTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTc                              >  7:217107/1‑149 (MQ=255)
                                                                                                             aataatTTAAATGAAATAAAAAAGACGGACAACTTAGTGCGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTc                              <  1:34848/149‑1 (MQ=255)
                                                                                                                 atTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTAt                          <  8:275283/149‑1 (MQ=255)
                                                                                                                  tttAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTAt                          >  8:344604/1‑148 (MQ=255)
                                                                                                                      aaTGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGAc                     <  8:387269/149‑1 (MQ=255)
                                                                                                                      aaTGAAATAAAAAAGACGGACAACTTAGTCGCTTGTCCGTCTTCATTATAACAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCCGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGAc                     >  2:263286/1‑149 (MQ=255)
                                                                                                                          aaaTAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTAc                 <  1:77141/149‑1 (MQ=255)
                                                                                                                              aaaaaaGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACaaaa             >  6:385706/1‑149 (MQ=255)
                                                                                                                              aaaaaaGACGCACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACaaaa             <  1:71335/149‑1 (MQ=255)
                                                                                                                                    gACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTAAGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcccccc       >  8:279424/1‑149 (MQ=255)
                                                                                                                                      cGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCgg     <  1:358957/149‑1 (MQ=255)
                                                                                                                                        gACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGc    >  8:183932/1‑148 (MQ=255)
                                                                                                                                         aCAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCg   >  2:65813/1‑148 (MQ=255)
                                                                                                                                         aCAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGa  <  7:237929/149‑1 (MQ=255)
                                                                                                                                           aaCTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTg                                     <  7:307546/112‑1 (MQ=255)
                                                                                                                                           aaCTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTg                                     >  8:307546/1‑112 (MQ=255)
                                                                                                                                                  tGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcc           <  5:75219/131‑1 (MQ=255)
                                                                                                                                                  tGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcc           >  6:75219/1‑131 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
AACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGA  >  NC_000913/900565‑900850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: